ACADVL (VLCAD) Double Nickase Plasmid (h): sc-403111-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACADVL Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACADVL Double Nickase Plasmid (h) and ACADVL Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACADVL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACADVL Antibody (H-7): sc-376239
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACADVL (VLCAD) Double Nickase Plasmid (h) | sc-403111-NIC | 20 µg | $410.00 | |||
ACADVL (VLCAD) Double Nickase Plasmid (h2) | sc-403111-NIC-2 | 20 µg | $410.00 |
ACADVL encodes very long-chain acyl-CoA dehydrogenase (VLCAD), a mitochondrial inner-membrane–associated flavoprotein that catalyzes the first dehydrogenation step of β-oxidation for long-chain fatty acyl-CoAs. By initiating mitochondrial fatty acid oxidation, VLCAD supports energy homeostasis during fasting and high-demand states and contributes to acetyl-CoA production for downstream metabolic integration with the TCA cycle and oxidative phosphorylation. ACADVL function is closely linked to lipid catabolism, mitochondrial redox balance, and cellular responses to nutrient availability. Disruption of ACADVL is associated with inherited disorders of long-chain fatty acid oxidation, making it a relevant locus for studying metabolic stress phenotypes and mitochondrial dysfunction mechanisms in human cells.
ACADVL Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACADVL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACADVL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACADVL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACADVL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.