ABC1 siRNA und shRNA Plasmide (h) human ABC1-spezifische siRNA, shRNA Plasmide und Lentivirale Partikel Gen Silencer

ABC1 siRNA und shRNA Plasmide (h)

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Datenblatt

    • Zielspezies: human; für das entsprechende mouse Produkt, verwenden Sie bitte ABC1 siRNA and shRNA Plasmids (m)
    • Gen Silencer Reagenzien bestehen in der Regel aus einem Gemisch aus drei bis fünf zielspezifischen 19-25 Nukleotide langen Sequenzen
    • Auf Anfrage zur unabhängigen Überprüfung der ABC1 Gen Silencing Ergebnisse sind die einzelnen Duplexkomponenten oder Plasmide ebenfalls erhältlich
    • Geeigneter Kontrollantikörper: ABC1 Antikörper (AC10): sc-53482 ist als Kontrollantikörper empfohlen, um die Herunterregulierung der ABC1 Expression per Western Blot oder Immunfluoreszenz zu überprüfen
    • Für weitere Informationen zur Beschreibung der si/shRNA Gen Silencer hier klicken
    • Gen Silencer Reagenzien sind ausschließlich für die nicht-kommerzielle, wissenschaftliche Verwendung bestimmt. Hier klicken für weitere Informationen.

Gen ID

SpeziesGenGen IDChromosomaler LocusmRNA (Isoform) Accession #Protein Accession #OMIM™ #
HumanABCA1199q31.1XM_005251773, XM_005251776, XM_011518339, XM_011518340, XM_011518341, XM_011518342, XM_011518344, XM_017014378, XM_017014379, XM_017014380, XM_017014381, XM_017014382, NM_005502O95477604091

Does all sc lentivirus have the same promoters? The reason I ask is that I have seen others cited using sc lentivirus successfully in Thp-1 cells for LPL. I heard that promoter selection is crucial for Thp-1 cells.

Gefragt von: weng
Thank you for your question. Yes, our vector uses an H1 promoter for shRNA transcription.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2019-04-23

I read the reference cited here. Is the shRNA targeting the same sequence? I want to make a stable knockdown of ABCA1 in Thp-1 cells. Does your shRNA guarantee knockdown? Does the product come with a positive control for transfection efficiency?

Gefragt von: weng
Thank you for your question. Yes, the ABC1 shRNA plasmid (h): sc-41136-SH is using the same target sequence as the siRNA product that is referenced in the product citation on our website. For stable knockdown, you may want to consider using the shRNA Lentiviral Particles. Please contact our Technical Service Department for more information. We do sell separately a positive control for measuring transfection efficiency, copGFP Control Plasmid: sc-108083. https://www.scbt.com/scbt/browse/shrna-plasmid-support-products/_/N-lhyfam Santa Cruz does warranty these si/shRNA reagents to show observable knockdown at the protein level.  Santa Cruz implements computationally designed published algorithms which are demonstrated to give greater than 50% knockdown more than 90% of the time. If you purchase the reagents and are not able to achieve the expected results we encourage you to contact our technical support staff.  If we are unable to trouble-shoot the assay further then we are able to offer you either research credit or refund the purchase price of the reagents.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2019-04-23
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