Alkaline Phosphatase (AP) (CAS 9001-78-9)
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Alternate Names:
cIAP; Orthophosphoric-monoester phosphohydrolase
Application:
Alkaline Phosphatase (AP) is a high activity grade enzyme that can be used for antibody and protein conjugation
CAS Number:
9001-78-9
Molecular Weight:
~140,000-160,000
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Alkaline Phosphatase (AP) is a dimeric glycoprotein. This enzyme requires calcium, magnesium, or zinc divalent ions for activity. The specificity of Alkaline Phosphatase (AP) is broad and includes phosphate esters of alcohols, amines, phenols, and pyrophosphates. Alkaline Phosphatase (AP) is often used to dephosphorylate proteins and nucleic acids. This product is supplied as a high activity grade and is recommended for antibody and protein conjugation. Activity range: 3,000-6,000 U/mg protein. Unit Definition: One unit will hydrolyze 1 µmole of p-nitrophenol from p-nitrophenyl-phosphate per minute at 37°C, pH 9.8 in diethanolamine buffer.
Alkaline Phosphatase (AP) (CAS 9001-78-9) References
- Clinical inquiries. What is the differential diagnosis of an elevated alkaline phosphatase (AP) level in an otherwise asymptomatic patient? | Reust, CE. and Hall, L. 2001. J Fam Pract. 50: 496-7. PMID: 11401735
- Plasma alkaline phosphatase is elevated in Alzheimer's disease and inversely correlates with cognitive function. | Kellett, KA., et al. 2011. Int J Mol Epidemiol Genet. 2: 114-21. PMID: 21686125
- QM/MM analysis suggests that Alkaline Phosphatase (AP) and nucleotide pyrophosphatase/phosphodiesterase slightly tighten the transition state for phosphate diester hydrolysis relative to solution: implication for catalytic promiscuity in the AP superfamily. | Hou, G. and Cui, Q. 2012. J Am Chem Soc. 134: 229-46. PMID: 22097879
- Persistently elevated alkaline phosphatase. | Verma, J. and Gorard, DA. 2012. BMJ Case Rep. 2012: PMID: 22922932
- Alkaline phosphatase: a marker of alveolar type II cell differentiation. | Edelson, JD., et al. 1988. Am Rev Respir Dis. 138: 1268-75. PMID: 2462386
- Pediatric reference intervals for alkaline phosphatase. | Zierk, J., et al. 2017. Clin Chem Lab Med. 55: 102-110. PMID: 27505090
- An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles. | Wang, Y., et al. 2021. Biosensors (Basel). 11: PMID: 33946723
- Alkaline Phosphatase Pathophysiology with Emphasis on the Seldom-Discussed Role of Defective Elimination in Unexplained Elevations of Serum ALP - A Case Report and Literature Review. | Levitt, MD., et al. 2022. Clin Exp Gastroenterol. 15: 41-49. PMID: 35313457
- Alkaline phosphatase in auxiliary liver transplantation. | Jersky, J. 1974. Gut. 15: 636-43. PMID: 4214064
- Serum alkaline phosphatase in hypophosphatasia. | Warshaw, JB., et al. 1971. J Clin Invest. 50: 2137-42. PMID: 5116205
- Neutrophil alkaline phosphatase in pregnancy. | Beal, RW., et al. 1967. J Clin Pathol. 20: 749-51. PMID: 5602987
- Alkaline phosphatase isoenzyme patterns in hyperthyroidism. | Cooper, DS., et al. 1979. Ann Intern Med. 90: 164-8. PMID: 582089
- Alkaline phosphatase and calcification, correlated or not? | Nijweide, PJ., et al. 1981. Metab Bone Dis Relat Res. 3: 61-6. PMID: 7266367
- Alkaline phosphatase isoenzymes. | Briere, RO. 1978. CRC Crit Rev Clin Lab Sci. 10: 1-30. PMID: 752443
- Alkaline phosphatase in human semen: an investigation using enzyme inhibitors and gel electrophoresis. | Lewin, LM., et al. 1993. Eur J Clin Chem Clin Biochem. 31: 811-4. PMID: 8136413
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Alkaline Phosphatase (AP), 1 mg | sc-3716 | 1 mg | $63.00 | |||
Alkaline Phosphatase (AP), 5 mg | sc-3716A | 5 mg | $218.00 |