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Coenzyme B12 (CAS 13870-90-1)

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Alternate Names:
Adenosyl cobalamine; 5′-Deoxyadenosylcobalamine; Cobamamide; DMBC coenzyme
CAS Number:
13870-90-1
Purity:
≥97%
Molecular Weight:
1579.58
Molecular Formula:
C72H100CoN18O17P
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Coenzyme B12 is a crucial cofactor in various enzymatic reactions, particularly in the conversion of methylmalonyl-CoA to succinyl-CoA and the conversion of homocysteine to methionine. It plays a significant role in the metabolism of fatty acids and amino acids, serving as a coenzyme for methylmalonyl-CoA mutase and methionine synthase. The mechanism of action involves the transfer of a methyl group from methyltetrahydrofolate to homocysteine, generating methionine and tetrahydrofolate. Additionally, coenzyme B12 facilitates the rearrangement of carbon skeletons in various metabolic pathways, contributing to the synthesis of essential molecules. Its functional role lies in its ability to facilitate these enzymatic reactions by serving as a cofactor, enabling the conversion of substrates into important metabolic intermediates.


Coenzyme B12 (CAS 13870-90-1) References

  1. Enzymatic radical catalysis: coenzyme B12-dependent diol dehydratase.  |  Toraya, T. 2002. Chem Rec. 2: 352-66. PMID: 12369058
  2. Coenzyme B12 riboswitches are widespread genetic control elements in prokaryotes.  |  Nahvi, A., et al. 2004. Nucleic Acids Res. 32: 143-50. PMID: 14704351
  3. Adenosyltransferase: an enzyme and an escort for coenzyme B12?  |  Yamanishi, M., et al. 2005. Trends Biochem Sci. 30: 304-8. PMID: 15950874
  4. The enzymatic activation of coenzyme B12.  |  Brown, KL. 2006. Dalton Trans. 1123-33. PMID: 16482346
  5. Coenzyme B12 controls transcription of the Streptomyces class Ia ribonucleotide reductase nrdABS operon via a riboswitch mechanism.  |  Borovok, I., et al. 2006. J Bacteriol. 188: 2512-20. PMID: 16547038
  6. Modeling the reactions catalyzed by coenzyme B12-dependent enzymes.  |  Sandala, GM., et al. 2010. Acc Chem Res. 43: 642-51. PMID: 20136160
  7. Multiple roles of ATP:cob(I)alamin adenosyltransferases in the conversion of B12 to coenzyme B12.  |  Mera, PE. and Escalante-Semerena, JC. 2010. Appl Microbiol Biotechnol. 88: 41-8. PMID: 20677021
  8. Biochemistry of B12-cofactors in human metabolism.  |  Kräutler, B. 2012. Subcell Biochem. 56: 323-46. PMID: 22116707
  9. Radical use of Rossmann and TIM barrel architectures for controlling coenzyme B12 chemistry.  |  Dowling, DP., et al. 2012. Annu Rev Biophys. 41: 403-27. PMID: 22577824
  10. Large-scale domain motions and pyridoxal-5'-phosphate assisted radical catalysis in coenzyme B12-dependent aminomutases.  |  Maity, AN., et al. 2014. Int J Mol Sci. 15: 3064-87. PMID: 24562332
  11. Coenzyme B12 can be produced by engineered Escherichia coli under both anaerobic and aerobic conditions.  |  Ko, Y., et al. 2014. Biotechnol J. 9: 1526-35. PMID: 25146562
  12. Coenzyme B12 Repurposed for Photoregulation of Gene Expression.  |  Gruber, K. and Kräutler, B. 2016. Angew Chem Int Ed Engl. 55: 5638-40. PMID: 27010518
  13. Coenzyme B12 synthesis as a baseline to study metabolite contribution of animal microbiota.  |  Danchin, A. and Braham, S. 2017. Microb Biotechnol. 10: 688-701. PMID: 28612402
  14. Analysis and characterization of coenzyme B12 biosynthetic gene clusters and improvement of B12 biosynthesis in Pseudomonas denitrificans ATCC 13867.  |  Nguyen-Vo, TP., et al. 2018. FEMS Microbiol Lett. 365: PMID: 30184199
  15. Biochemistry of coenzyme B12-dependent glycerol and diol dehydratases and organization of the encoding genes.  |  Daniel, R., et al. 1998. FEMS Microbiol Rev. 22: 553-66. PMID: 9990728

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Coenzyme B12, 10 mg

sc-214754
10 mg
$50.00

Coenzyme B12, 25 mg

sc-214754A
25 mg
$77.00

Coenzyme B12, 100 mg

sc-214754B
100 mg
$149.00

Coenzyme B12, 1 g

sc-214754C
1 g
$941.00

Coenzyme B12, 10 g

sc-214754D
10 g
$4165.00