ZNF784 Activators
ZNF784 can influence its function through various biochemical pathways primarily by altering its phosphorylation state. Forskolin, known for its ability to stimulate adenylyl cyclase, increases cyclic AMP levels within cells, which in turn activates protein kinase A (PKA). Activated PKA is known to phosphorylate a multitude of proteins, and it can target ZNF784, potentially enhancing its activity as a transcription factor. Similarly, isoproterenol and epinephrine, both adrenergic agonists, raise intracellular cAMP levels, leading to PKA activation. This cascade of activation can result in the phosphorylation of ZNF784, which may amplify its role in regulating gene expression. Cholera toxin operates by permanently activating the Gs alpha protein, resulting in sustained elevation of cAMP and consequent PKA activation, which could also phosphorylate ZNF784. Dibutyryl-cAMP, a synthetic analog of cAMP, bypasses cell surface receptors and directly activates PKA, offering another route to ZNF784 phosphorylation.
PMA, an activator of protein kinase C (PKC), triggers a phosphorylation cascade that can enhance ZNF784's DNA binding activity or its interaction with other regulatory proteins. Ionomycin, by raising intracellular calcium levels, activates calmodulin-dependent protein kinases that may subsequently phosphorylate ZNF784. Thapsigargin, which disrupts calcium homeostasis by inhibiting SERCA, can lead to a similar activation of calcium-dependent pathways affecting ZNF784. Anisomycin exerts its influence by activating stress-activated protein kinases like JNK, which may target ZNF784 for phosphorylation. Calyculin A and okadaic acid both act to sustain the phosphorylation state of proteins by inhibiting phosphatases that would normally reverse phosphorylation; this action can keep ZNF784 in an active state. H-89, while being a PKA inhibitor, can paradoxically lead to increased PKA activity through feedback mechanisms, potentially enhancing ZNF784 phosphorylation. These chemical activators, through their varied effects on cellular signaling pathways, can modulate the activity of ZNF784 by influencing its phosphorylation status.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly stimulates adenylyl cyclase, leading to an increase in cyclic AMP (cAMP) levels, which can activate protein kinase A (PKA). PKA phosphorylation can lead to activation of ZNF784 by phosphorylating sites that regulate its transcriptional activity. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
Phorbol 12-myristate 13-acetate (PMA) activates protein kinase C (PKC), which can phosphorylate transcription factors and other proteins. PKC-mediated phosphorylation could enhance ZNF784's DNA binding activity or its interaction with other regulatory proteins. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that raises intracellular calcium levels, which can activate calmodulin-dependent protein kinases. These kinases may phosphorylate ZNF784, potentially increasing its DNA binding affinity and transcriptional regulatory functions. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable cAMP analog that activates PKA. Activated PKA can then phosphorylate ZNF784, which may enhance its function as a transcription factor by increasing its nuclear localization or DNA binding capacity. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $27.00 $37.00 | 5 | |
Isoproterenol is a beta-adrenergic agonist that stimulates adenylyl cyclase, raising cAMP levels and activating PKA. PKA activation can phosphorylate ZNF784, potentially leading to increased transcriptional activity. | ||||||
(−)-Epinephrine | 51-43-4 | sc-205674 sc-205674A sc-205674B sc-205674C sc-205674D | 1 g 5 g 10 g 100 g 1 kg | $40.00 $102.00 $197.00 $1739.00 $16325.00 | ||
Epinephrine interacts with adrenergic receptors to increase intracellular cAMP, which activates PKA. PKA may target ZNF784 for phosphorylation, thereby enhancing its transcription factor activity. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that can activate stress-activated protein kinases like JNK. JNK activation could lead to phosphorylation of ZNF784, potentially enhancing its regulatory effects on gene expression. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), causing an increase in cytosolic calcium levels which can activate pathways including calmodulin-dependent kinases, potentially leading to ZNF784 activation via phosphorylation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A is a potent inhibitor of protein phosphatases 1 and 2A, leading to increased phosphorylation levels of cellular proteins. Inhibition of dephosphorylation could maintain ZNF784 in an activated phosphorylated state. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid is a specific inhibitor of protein phosphatases PP1 and PP2A, which leads to hyperphosphorylation of cellular proteins. This may result in the sustained activation of ZNF784 due to reduced dephosphorylation. | ||||||