RPL12 Inhibitors
Chemical inhibitors of RPL12 function through a variety of mechanisms to disrupt the protein's role in the assembly and function of the ribosome, a critical cellular machine responsible for synthesizing proteins. For instance, Cycloheximide targets the translocation step in protein synthesis, essentially freezing the ribosome's ability to move along the messenger RNA (mRNA), which indirectly compromises RPL12's function within the ribosomal complex. Ricin, a notoriously toxic substance, inactivates the 60S ribosomal subunit by damaging the rRNA essential for RPL12's binding and proper ribosome function. Alpha-sarcin acts with precision to cleave a specific bond within rRNA, which, much like ricin, impairs the ribosomal function and thus inhibits RPL12. Anisomycin binds to the 60S subunit, inhibiting peptidyl transferase activity, and Emetine attaches itself to the 40S subunit, obstructing the translocation process. Both of these actions disrupt the normal functioning of RPL12 within the ribosome.
Further, Harringtonine and its derivative Homoharringtonine prevent the proper elongation of the nascent protein chain, thus blocking RPL12 from fulfilling its role in protein synthesis. Pactamycin disrupts the formation of the initiation complex, essential for the assembly of new ribosomes, including the integration of RPL12. Puromycin, by causing premature chain termination, leads to dysfunctional protein synthesis, which also affects RPL12's functional contribution to the ribosome. Sparsomycin and Streptovitacin A target the ribosome's peptidyl transferase center, with the former inhibiting peptide bond formation and the latter interfering with translocation. Both of these actions compromise RPL12's role in catalysis within the ribosome. Finally, Tunicamycin, by inhibiting N-linked glycosylation and the formation of the first peptide bond, impacts the overall ribosome structure and function, including that of RPL12. Each of these chemicals, by targeting different steps or aspects of the protein synthesis process, effectively inhibits the normal function of RPL12 within the ribosome.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Cycloheximide | 66-81-9 | sc-3508B sc-3508 sc-3508A | 100 mg 1 g 5 g | $41.00 $84.00 $275.00 | 127 | |
Cycloheximide inhibits protein biosynthesis in eukaryotes by interfering with the translocation step in protein synthesis, thereby indirectly inhibiting the function of RPL12 by preventing its participation in the assembly and function of the ribosome. | ||||||
α-Sarcin | 86243-64-3 | sc-204427 | 1 mg | $462.00 | 6 | |
Alpha-sarcin is a fungal ribotoxin that cleaves a specific phosphodiester bond within the ribosomal RNA of the large ribosomal subunit, hence inhibiting RPL12 by impairing ribosomal function and protein synthesis. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is an antibiotic that inhibits eukaryotic protein synthesis by binding to the 60S ribosomal subunit and inhibiting peptidyl transferase activity, thereby inhibiting the function of RPL12 as part of the ribosome. | ||||||
Emetine | 483-18-1 | sc-470668 sc-470668A sc-470668B sc-470668C | 1 mg 10 mg 50 mg 100 mg | $440.00 $900.00 $1400.00 $2502.00 | ||
Emetine acts as an inhibitor of protein synthesis by binding to the 40S ribosomal subunit and inhibiting the translocation of the peptidyl-tRNA from the A-site to the P-site, which can inhibit RPL12 by affecting ribosomal activity and thus protein synthesis. | ||||||
Harringtonin | 26833-85-2 | sc-204771 sc-204771A sc-204771B sc-204771C sc-204771D | 5 mg 10 mg 25 mg 50 mg 100 mg | $250.00 $367.00 $548.00 $730.00 $980.00 | 30 | |
Harringtonine is a cephalotaxine ester that inhibits protein synthesis by preventing the initial elongation step of translation, which can inhibit RPL12 by blocking its incorporation into functional ribosomes and thus interfering with its role in protein synthesis. | ||||||
Homoharringtonine | 26833-87-4 | sc-202652 sc-202652A sc-202652B | 1 mg 5 mg 10 mg | $52.00 $125.00 $182.00 | 11 | |
Homoharringtonine, a derivative of harringtonine, inhibits protein synthesis by blocking the peptidyl transferase reaction, which can inhibit RPL12 by preventing its normal function in the ribosome during translation. | ||||||
Puromycin | 53-79-2 | sc-205821 sc-205821A | 10 mg 25 mg | $166.00 $322.00 | 436 | |
Puromycin is an aminonucleoside antibiotic that causes premature chain termination during protein synthesis by acting as an analog of aminoacyl-tRNA, which can inhibit RPL12 by leading to dysfunctional protein synthesis within the ribosome. | ||||||
Tunicamycin | 11089-65-9 | sc-3506A sc-3506 | 5 mg 10 mg | $172.00 $305.00 | 66 | |
Tunicamycin is an antibiotic that inhibits N-linked glycosylation and can also inhibit protein synthesis by blocking the formation of the first peptide bond. This inhibition impacts the overall structure and function of the ribosome, which includes the functional inhibition of RPL12 as it cannot properly engage in protein synthesis without correct ribosome assembly. | ||||||