rotatin Activators
Rotatin is a protein implicated in several critical cellular processes, including the organization of the microtubule network, intracellular transport mechanisms, and the regulation of ciliogenesis. Its activation is essential for the proper execution of these functions, which are pivotal in maintaining cellular architecture and signaling pathways. The precise mechanisms through which rotatin exerts its effects involve complex interactions with other cellular components and are influenced by its phosphorylation state, which can be modulated by various signaling molecules. The chemicals listed above, through different pathways, all converge on the activation of cAMP and PKA signaling cascades, which are known to phosphorylate and activate proteins, including rotatin. This underscores the intricate network of cellular signaling pathways that regulate rotatin's activity and highlights the potential of these chemicals to influence rotatin function in a cellular context.
The activation of rotatin is not a straightforward process but is mediated through a series of upstream signaling events that ultimately lead to its functional engagement in cellular processes. The chemicals provided, each with a unique mechanism of action, exemplify the diversity of approaches that can be utilized to indirectly activate rotatin. Through the modulation of cAMP levels, either by direct activation of adenylyl cyclase, inhibition of phosphodiesterases, or through receptor-mediated pathways, these chemicals enhance PKA activity. PKA, in turn, phosphorylates rotatin, a post-translational modification that is crucial for its activation. This phosphorylation is a regulatory mechanism that controls rotatin's activity, facilitating its role in essential cellular functions such as the maintenance of the microtubule network, which is vital for cell shape, organelle positioning, and intracellular transport, as well as in the formation and function of cilia, which are critical for cell and fluid movement across cell surfaces.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylyl cyclase, increasing cAMP levels. Elevated cAMP activates PKA (Protein Kinase A), which can phosphorylate and thus activate rotatin. This activation enhances rotatin's function in cellular processes, such as intracellular transport and ciliogenesis. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
IBMX, a non-selective inhibitor of phosphodiesterases, prevents cAMP degradation, indirectly maintaining high levels of cAMP. This sustains PKA activity, promoting phosphorylation and activation of rotatin, which is crucial for its role in maintaining cellular architecture. | ||||||
PGE2 | 363-24-6 | sc-201225 sc-201225C sc-201225A sc-201225B | 1 mg 5 mg 10 mg 50 mg | $56.00 $156.00 $270.00 $665.00 | 37 | |
PGE2, through its receptor EP2, stimulates cAMP production. The consequent PKA activation may enhance rotatin phosphorylation and activation, supporting its role in cellular signaling and structure maintenance. | ||||||
Rolipram | 61413-54-5 | sc-3563 sc-3563A | 5 mg 50 mg | $75.00 $212.00 | 18 | |
Rolipram inhibits PDE4, leading to increased cAMP levels in cells. Elevated cAMP activates PKA, which can phosphorylate rotatin, thereby potentially enhancing its cellular functions, including its involvement in microtubule organization. | ||||||
Adenosine 3′,5′-cyclic monophosphate | 60-92-4 | sc-217584 sc-217584A sc-217584B sc-217584C sc-217584D sc-217584E | 100 mg 250 mg 5 g 10 g 25 g 50 g | $114.00 $175.00 $260.00 $362.00 $617.00 $1127.00 | ||
Dibutyryl-cAMP is a cell-permeable cAMP analog that directly activates cAMP-dependent pathways. This activation includes PKA, which may phosphorylate and activate rotatin, influencing its cellular roles such as cytoskeletal organization. | ||||||
(−)-Epinephrine | 51-43-4 | sc-205674 sc-205674A sc-205674B sc-205674C sc-205674D | 1 g 5 g 10 g 100 g 1 kg | $40.00 $102.00 $197.00 $1739.00 $16325.00 | ||
Epinephrine, through β-adrenergic receptors, increases cAMP levels, activating PKA. PKA may then activate rotatin by phosphorylation, implicating epinephrine in modulating rotatin-dependent cellular responses, such as signal transduction mechanisms. | ||||||
Zaprinast (M&B 22948) | 37762-06-4 | sc-201206 sc-201206A | 25 mg 100 mg | $103.00 $245.00 | 8 | |
Zaprinast, a PDE5 inhibitor, increases cGMP levels, which can indirectly elevate cAMP, leading to PKA activation. This may result in rotatin activation via phosphorylation, implicating zaprinast in rotatin's role in cellular signaling and structure. | ||||||
Milrinone | 78415-72-2 | sc-201193 sc-201193A | 10 mg 50 mg | $162.00 $683.00 | 7 | |
Milrinone inhibits PDE3, increasing cAMP levels and thereby activating PKA. PKA, in turn, may phosphorylate and activate rotatin, suggesting milrinone's influence on rotatin's involvement in cellular homeostasis and signaling. | ||||||
Cilostamide (OPC 3689) | 68550-75-4 | sc-201180 sc-201180A | 5 mg 25 mg | $90.00 $350.00 | 16 | |
Cilostamide, a selective PDE3 inhibitor, elevates cAMP levels, leading to PKA activation. This activation potentially facilitates rotatin phosphorylation and activation, highlighting cilostamide's role in modulating rotatin's cellular functions, including its participation in ciliogenesis. | ||||||