ORC5 Activators
ORC5 Activators are chemical entities that indirectly bolster the functional activity of ORC5, a pivotal component in the initiation of DNA replication. ATP, fundamental to cellular processes, specifically enhances ORC5's activity by supplying the energy critical for the Origin Recognition Complex (ORC) to load helicase, thereby initiating replication. Caffeine intercepts phosphodiesterase, elevating cAMP levels, which indirectly promotes ORC5 function through PKA activation and subsequent stabilization of ORC5 interactions. Thymidine and Hydroxyurea, which, by instigating an S-phase checkpoint and depleting nucleotide pools, respectively, enhance ORC5's involvement in replication initiation and fork stabilization.
Further indirect enhancement of ORC5 is facilitated by chemicals that influence DNA integrity and the replication stress response. The ATR and CHK1 inhibitors, VE-821 and AZD7762, deregulate checkpoints leading to increased replication initiation, indirectly necessitating ORC5's activity. Moreover, inhibition of CDKs with Roscovitine alters replication licensing, potentially increasing the demand for ORC5's replication initiation function. The MCM Helicase Inhibitor acts to induce replication stress, thereby amplifying ORC5's activity to ensure replication licensing and origin stabilization.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Adenosine 5′-Triphosphate, disodium salt | 987-65-5 | sc-202040 sc-202040A | 1 g 5 g | $38.00 $74.00 | 9 | |
ATP is required as an energy source for the ORC to initiate DNA replication. Binding of ATP to ORC5 is crucial for ORC's helicase loading activity, indirectly enhancing ORC5's function in DNA replication initiation. | ||||||
Caffeine | 58-08-2 | sc-202514 sc-202514A sc-202514B sc-202514C sc-202514D | 5 g 100 g 250 g 1 kg 5 kg | $32.00 $66.00 $95.00 $188.00 $760.00 | 13 | |
Caffeine can inhibit phosphodiesterase, leading to an increase in cyclic AMP (cAMP), which can enhance protein kinase A (PKA) activity. PKA phosphorylates multiple substrates that could indirectly stabilize ORC5's interaction with other ORC subunits or replication origins. | ||||||
Thymidine | 50-89-5 | sc-296542 sc-296542A sc-296542C sc-296542D sc-296542E sc-296542B | 1 g 5 g 100 g 250 g 1 kg 25 g | $48.00 $72.00 $265.00 $449.00 $1724.00 $112.00 | 16 | |
Thymidine is a nucleoside that, when in excess, can lead to DNA replication stress and an S-phase checkpoint response. This may indirectly result in augmented ORC5 activity due to increased checkpoint signaling to ensure proper replication initiation. | ||||||
Hydroxyurea | 127-07-1 | sc-29061 sc-29061A | 5 g 25 g | $76.00 $255.00 | 18 | |
Hydroxyurea induces replication stress by depleting deoxyribonucleotide pools, potentially leading to enhanced ORC5 involvement in stabilizing replication forks and re-initiation events. | ||||||
VE 821 | 1232410-49-9 | sc-475878 | 10 mg | $360.00 | ||
ATR is a critical kinase in the DNA damage response. Inhibition of ATR can increase replication initiation events as a compensatory mechanism, potentially enhancing ORC5 activity. | ||||||
AZD7762 | 860352-01-8 | sc-364423 | 2 mg | $107.00 | ||
CHK1 is another key kinase in the DNA damage response. Inhibiting CHK1 can lead to enhanced initiation of replication to bypass replication stress, indirectly enhancing ORC5 function. | ||||||
Roscovitine | 186692-46-6 | sc-24002 sc-24002A | 1 mg 5 mg | $92.00 $260.00 | 42 | |
CDKs regulate the cell cycle and replication initiation. Inhibiting certain CDKs can lead to alterations in the replication licensing process, potentially increasing ORC5 activity to maintain replication timing. | ||||||