OOSP1 Activators
OOSP1 employ diverse mechanisms to modulate the activity of this protein. Forskolin serves as a direct stimulant of adenylate cyclase, which in turn catalyzes the conversion of ATP to cyclic AMP (cAMP). Elevated cAMP levels are instrumental in activating protein kinase A (PKA), a kinase capable of phosphorylating various substrates, including OOSP1. This phosphorylation event by PKA can alter OOSP1's activity, leading to its activation. Similarly, IBMX functions by inhibiting phosphodiesterases, the enzymes responsible for cAMP degradation. Through its inhibitory action, IBMX sustains high levels of cAMP within the cell, thereby indirectly supporting the PKA-mediated phosphorylation of OOSP1. Dibutyryl-cAMP, a cell-permeable cAMP analog, bypasses the cellular controls on cAMP production and directly engages PKA signaling pathways, which can result in the phosphorylation and consequent activation of OOSP1.
PMA activates protein kinase C (PKC), which could phosphorylate OOSP1 if it is a PKC substrate. This provides an alternative phosphorylation route independent of the cAMP-PKA axis. Ionomycin, by increasing intracellular calcium levels, can activate calcium-dependent kinases that are capable of targeting OOSP1 for phosphorylation. Epinephrine binds to adrenergic receptors and activates cAMP-dependent signaling cascades, further potentiating PKA activity and the downstream phosphorylation of OOSP1. Anisomycin, known for its role in activating stress-activated protein kinases like JNK, can also lead to the activation of OOSP1 should it be a target within the JNK signaling pathway. Inhibitors of protein phosphatases such as Calyculin A and Okadaic Acid create an environment of increased phosphorylation by preventing dephosphorylation, which may result in the sustained activation of OOSP1. Lastly, agents like Thapsigargin and Zinc Pyrithione disrupt intracellular calcium signaling and MAPK pathways, respectively, which may influence the phosphorylation state and activity of OOSP1. These chemicals together provide a multifaceted approach to modulating the phosphorylation status and activity of OOSP1.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly stimulates adenylate cyclase, increasing cyclic AMP (cAMP) levels in cells. Elevated cAMP activates PKA (protein kinase A), which can phosphorylate and thereby activate OOSP1, assuming OOSP1 is a substrate for PKA. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
Isobutylmethylxanthine (IBMX) inhibits phosphodiesterases, enzymes that break down cAMP. By preventing cAMP degradation, IBMX indirectly maintains high levels of cAMP which can enhance PKA activity, potentially leading to OOSP1 activation. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
Phorbol 12-myristate 13-acetate (PMA) activates protein kinase C (PKC). If OOSP1 is a substrate for PKC, activation of PKC by PMA could lead to the phosphorylation and activation of OOSP1. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Elevated calcium can activate calmodulin-dependent kinases, which could phosphorylate and activate OOSP1 if OOSP1 is sensitive to such regulation. | ||||||
(−)-Epinephrine | 51-43-4 | sc-205674 sc-205674A sc-205674B sc-205674C sc-205674D | 1 g 5 g 10 g 100 g 1 kg | $40.00 $102.00 $197.00 $1739.00 $16325.00 | ||
Epinephrine binds to adrenergic receptors, leading to the activation of cAMP-dependent pathways and increased PKA activity. PKA can then activate OOSP1 by phosphorylation if OOSP1 is within PKA's substrate profile. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is known to activate stress-activated protein kinases like JNK. Activation of these kinases could lead to the phosphorylation and activation of OOSP1 if it is a part of the JNK signaling pathway. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A is an inhibitor of protein phosphatases 1 (PP1) and 2A (PP2A), which can result in increased phosphorylation levels of proteins due to reduced dephosphorylation. This could lead to sustained activation of OOSP1. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Similar to Calyculin A, Okadaic Acid is a potent inhibitor of PP1 and PP2A, leading to hyperphosphorylation of proteins. This may result in the activation of OOSP1 due to decreased dephosphorylation rates. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable analog of cAMP that activates cAMP-dependent pathways such as PKA. Activation of PKA could result in the phosphorylation and activation of OOSP1 if OOSP1 is a target of PKA. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to an increase in cytosolic calcium levels. This can activate calcium-dependent kinases, which might then activate OOSP1 through phosphorylation. | ||||||