Date published: 2025-9-5

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HRP and AP Substrates

Santa Cruz Biotechnology now offers a broad range of HRP and AP substrates for use in various applications. HRP (horseradish peroxidase) and AP (alkaline phosphatase) substrates are essential reagents in immunoassays and molecular biology techniques, where they are used to detect the presence of specific proteins, nucleic acids, or other biomolecules. These substrates react with their respective enzymes, HRP or AP, to produce a detectable signal, typically colorimetric, chemiluminescent, or fluorescent, which indicates the presence and quantity of the target molecule. Researchers use HRP and AP substrates extensively in applications such as Western blotting, ELISA, immunohistochemistry, and in situ hybridization. These substrates are crucial for achieving high sensitivity and specificity in these assays, enabling the detection of low-abundance targets and providing quantitative and qualitative data on biomolecular interactions. By offering a comprehensive selection of high-quality HRP and AP substrates, Santa Cruz Biotechnology supports advanced research in biochemistry, cell biology, and diagnostic development. These products empower scientists to conduct precise and reproducible experiments, driving discoveries and innovations in understanding biological processes and developing new analytical methods. View detailed information on our available HRP and AP substrates by clicking on the product name.

Items 31 to 32 of 32 total

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Product NameCAS #Catalog #QUANTITYPriceCitationsRATING

Horseradish peroxidase (HRP), RZ 1.0

9003-99-0sc-280786
sc-280786A
100 mg
250 mg
$149.00
$281.00
6
(1)

Horseradish peroxidase (HRP), RZ 1.0, is a versatile enzyme known for its exceptional catalytic efficiency in redox reactions. Its unique heme group facilitates electron transfer, enabling rapid oxidation of various substrates. The enzyme's structure allows for specific binding interactions, enhancing substrate specificity and reaction rates. Additionally, HRP exhibits remarkable stability across a range of pH levels, making it suitable for diverse biochemical applications while maintaining consistent activity.

Naphthol AS-BI-phosphate

1919-91-1sc-222042
sc-222042A
100 mg
1 g
$96.00
$282.00
1
(1)

Naphthol AS-BI-phosphate serves as a substrate for alkaline phosphatase (AP) and horseradish peroxidase (HRP), showcasing unique reactivity due to its phosphoric acid ester functionality. This compound undergoes hydrolysis, releasing naphthol, which can participate in subsequent coupling reactions. Its structural features promote selective interactions with enzymes, influencing reaction kinetics and enhancing colorimetric detection methods. The compound's solubility and stability in various conditions further optimize its performance in enzymatic assays.