HRP and AP Substrates

TMB Substrate Buffer, 1X, is a chromogenic substrate that exhibits a distinct affinity for horseradish peroxidase (HRP) and alkaline phosphatase (AP). Its unique electron-donating properties facilitate the formation of a colored product upon enzymatic reaction, allowing for sensitive detection. The buffer's pH stability and optimal ionic strength enhance enzyme activity, while its ability to undergo rapid oxidation contributes to efficient signal amplification in assays.

DAB, 50X, serves as a potent chromogenic substrate for horseradish peroxidase (HRP) and alkaline phosphatase (AP), characterized by its ability to form a stable, colored precipitate upon enzymatic action. Its unique structural features promote specific interactions with enzyme active sites, enhancing reaction kinetics. The compound's hydrophobic nature aids in solubility and diffusion, ensuring effective substrate availability, while its robust stability under varying conditions supports consistent assay performance.

PNPP, Disodium Salt, is a versatile chromogenic substrate that exhibits remarkable specificity for horseradish peroxidase (HRP) and alkaline phosphatase (AP). Its unique molecular structure facilitates efficient enzyme-substrate interactions, leading to rapid reaction kinetics. The compound's ionic nature enhances solubility in aqueous environments, promoting effective diffusion. Additionally, its stability across a range of pH levels ensures reliable performance in various biochemical assays.

BCIP/NBT Stock Solution, 50X, is a potent chromogenic substrate that demonstrates exceptional reactivity with horseradish peroxidase (HRP) and alkaline phosphatase (AP). Its unique electron-rich structure allows for specific redox reactions, resulting in a vivid colorimetric change. The compound's ability to form stable complexes with metal ions enhances its sensitivity, while its low background noise ensures clear signal detection in various biochemical applications.

AEC Substrate Buffer, 10X, is a versatile reagent that facilitates enzymatic reactions involving horseradish peroxidase (HRP) and alkaline phosphatase (AP). Its unique formulation promotes optimal pH stability, enhancing enzyme activity and reaction kinetics. The buffer's specific ionic interactions contribute to improved substrate solubility and reactivity, allowing for precise control over enzymatic pathways. This results in reliable and reproducible colorimetric outputs in various assays.

3,3'-Diaminobenzidine tetrahydrochloride is a potent chromogenic substrate that exhibits remarkable specificity in enzymatic reactions with horseradish peroxidase (HRP) and alkaline phosphatase (AP). Its unique electron-donating properties facilitate rapid oxidation, leading to the formation of a stable, colored precipitate. The compound's ability to form strong hydrogen bonds enhances its interaction with enzyme active sites, optimizing reaction rates and improving sensitivity in detection assays.

3,3'-Diaminobenzidine is a versatile chromogenic substrate known for its exceptional reactivity with horseradish peroxidase (HRP) and alkaline phosphatase (AP). Its structure allows for efficient electron transfer, promoting swift enzymatic oxidation. The compound's planar configuration enhances π-π stacking interactions, which can stabilize enzyme-substrate complexes. This results in increased reaction kinetics and improved signal amplification in various biochemical assays.

BCIP/NBT-Blue Liquid Substrate System is a highly sensitive chromogenic substrate that exhibits unique interactions with horseradish peroxidase (HRP) and alkaline phosphatase (AP). Its distinct chemical structure facilitates the formation of a blue precipitate through a series of redox reactions, enhancing visual contrast in assays. The substrate's solubility and stability in aqueous environments contribute to consistent performance, while its ability to generate a stable colorimetric signal allows for precise quantification in various analytical applications.

BCIP/INT, 1X is a versatile chromogenic substrate that engages in specific electron transfer mechanisms with horseradish peroxidase (HRP) and alkaline phosphatase (AP). Its unique molecular architecture promotes rapid reaction kinetics, leading to the formation of an intense purple product. This substrate's exceptional solubility and compatibility with diverse assay conditions ensure reliable performance, making it ideal for generating distinct colorimetric signals in various biochemical analyses.

PPHP serves as a versatile substrate for horseradish peroxidase (HRP) and alkaline phosphatase (AP), characterized by its unique ability to engage in specific molecular interactions that enhance enzymatic activity. Its distinct chemical structure promotes rapid electron transfer, leading to accelerated reaction kinetics. Additionally, PPHP's stability under diverse conditions allows for consistent product formation, making it an essential tool in various biochemical assays for reliable analytical results.