HECTD4 Activators
The chemical class known as HECTD4 Activators encompasses a diverse group of compounds that can modulate the activity of the HECT domain-containing E3 ubiquitin ligase 4 (HECTD4). The activation of HECTD4 is not tied to direct binding interactions with small molecules, as is common with enzyme activators or receptor agonists, but rather through indirect modulation of cellular signaling pathways and post-translational modifications that can ultimately influence the ubiquitin ligase activity of HECTD4. This activation can involve changes in the phosphorylation status of proteins within the cell, either by promoting kinase activity or inhibiting phosphatase activity, thereby altering protein interactions or functions that are upstream of HECTD4. Increases in cellular ubiquitin levels due to proteasome inhibition can also play a role in the activation of HECTD4 by ensuring a sufficient supply of ubiquitin for tagging substrates.
This class of compounds can also affect intracellular messenger levels, such as cAMP, or modulate the activity of key signaling molecules that partake in cascades relevant to the regulation of HECTD4. The activation of protein kinase C (PKC) and alterations in calcium signaling are examples of such upstream events that can lead to changes in the cellular environment conducive to the activation of HECTD4. Phosphorylation state changes due to the action of these compounds can result in the modification of proteins that are either substrates or regulators of HECTD4, thereby enhancing its E3 ligase activity. The ubiquitination process itself, pivotal for protein turnover and signaling, is thus indirectly influenced by these compounds through various levels of cellular regulation and signaling events.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylyl cyclase, increasing intracellular levels of cAMP. Elevated cAMP could activate PKA activity, which could phosphorylate proteins that interact with or regulate HECTD4, potentially enhancing its E3 ligase activity. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $56.00 $260.00 $980.00 | 163 | |
MG132 is a proteasome inhibitor. By inhibiting the degradation of ubiquitinated proteins, MG132 could lead to increased cellular ubiquitin levels, which may enhance the ubiquitination activity of HECTD4 by substrate accumulation. | ||||||
Epoxomicin | 134381-21-8 | sc-201298C sc-201298 sc-201298A sc-201298B | 50 µg 100 µg 250 µg 500 µg | $134.00 $215.00 $440.00 $496.00 | 19 | |
Another proteasome inhibitor, Epoxomicin, could similarly increase the pool of ubiquitinated proteins and ubiquitin, which could enhance HECTD4's activity by substrate feedback. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases PP1 and PP2A. By preventing dephosphorylation, it could maintain proteins in a phosphorylated state that favors HECTD4 interaction or activation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Similar to okadaic acid, calyculin A inhibits protein phosphatases, which could enhance HECTD4's ubiquitination activity by maintaining or altering phosphorylation states of proteins within HECTD4's pathway. | ||||||
Sodium Orthovanadate | 13721-39-6 | sc-3540 sc-3540B sc-3540A | 5 g 10 g 50 g | $45.00 $56.00 $183.00 | 142 | |
This is a phosphatase inhibitor that can prevent dephosphorylation. By doing so, it could enhance HECTD4 activity if HECTD4 is regulated by phosphorylation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that can raise intracellular calcium levels. As calcium signaling can influence many cellular processes, including some E3 ligase activities, it could affect HECTD4 function. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC). PKC-mediated phosphorylation could alter the function or interactions of HECTD4, thereby potentially enhancing its activity. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is a potent inhibitor of the PI3K/Akt pathway. By modulating this pathway, it could alter the phosphorylation and activity of proteins that regulate HECTD4 activity. | ||||||
U-0126 | 109511-58-2 | sc-222395 sc-222395A | 1 mg 5 mg | $63.00 $241.00 | 136 | |
U0126 is an inhibitor of MEK1/2, which are upstream of ERK in the MAPK pathway. By modulating this pathway, it can influence the activity of downstream proteins that could interact with or regulate HECTD4. | ||||||