eIF3M Activators
The functional activity of the protein eIF3M is enhanced through various chemical compounds that interact with specific signaling pathways and cellular processes. For instance, Methotrexate increases the requirement for one-carbon units, indirectly stimulating the protein synthesis machinery inwhich eIF3M is integral, leading to its increased functional activity. Brefeldin A induces ER stress and the unfolded protein response, which escalates the demand for translation initiation, thereby potentially augmenting eIF3M's role. Similarly, Cycloheximide and Puromycin, by inhibiting translation elongation and causing premature chain termination respectively, create a feedback loop that can enhance the activity of eIF3M, as the cell attempts to initiate more translation to compensate for the loss of protein synthesis. Anisomycin, by disrupting peptide bond formation, is another stress inducer that can lead to an upregulation of eIF3M's activity to facilitate the increased rate of translation initiation needed under such stress conditions.
Furthermore, Salubrinal's inhibition of eIF2α dephosphorylation and ISRIB's modulation of the stress response indirectly elevate eIF3M's activity by enhancing the initiation of protein synthesis. Rapamycin indirectly contributes to the upregulation of cap-independent translation initiation mechanisms, where eIF3M could be increasingly active. Autophagy induced by Spermidine might increase eIF3M activity by degrading translation repressors. Tunicamycin triggers ER stress that could enhance eIF3M's activity as part of a cellular response to increase protein folding capacity. MG132 causes accumulation of ubiquitinated proteins, stressing the cell and potentially enhancing eIF3M's role in initiating translation to mitigate protein misfolding. Lastly, Homoharringtonine's inhibition of translation elongation may result in a compensatory upregulation of translation initiation, thereby increasing eIF3M's functional activity. These chemical activators, through their targeted actions, collectively facilitate the enhancement of eIF3M-mediated functions associated with the initiation of protein synthesis.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Methotrexate | 59-05-2 | sc-3507 sc-3507A | 100 mg 500 mg | $94.00 $213.00 | 33 | |
Methotrexate acts as a dihydrofolate reductase inhibitor, leading to the accumulation of dihydrofolate and a subsequent increase in the need for one-carbon units. This heightened demand can enhance the activity of eIF3M, which is involved in the initiation of translation, by necessitating greater protein synthesis capacity. | ||||||
Brefeldin A | 20350-15-6 | sc-200861C sc-200861 sc-200861A sc-200861B | 1 mg 5 mg 25 mg 100 mg | $31.00 $53.00 $124.00 $374.00 | 25 | |
Brefeldin A disrupts ER to Golgi transport, which can lead to ER stress and the unfolded protein response (UPR). The UPR activates signaling pathways that enhance translation initiation, a step in which eIF3M is critically involved, to compensate for the misfolded proteins. | ||||||
Puromycin | 53-79-2 | sc-205821 sc-205821A | 10 mg 25 mg | $166.00 $322.00 | 436 | |
Puromycin causes premature chain termination during translation by acting as an analog of the aminoacyl-tRNA. This can cause a feedback increase in the need for translation initiation, potentially increasing the functional activity of eIF3M as the cell attempts to compensate for the loss of protein synthesis. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin interferes with peptide bond formation at the ribosome. This stressor can lead to a compensatory increase in translation initiation, potentially enhancing the functional activity of eIF3M by increasing the rate at which initiation and ribosome assembly occurs. | ||||||
ISRIB | 1597403-47-8 | sc-488404 | 10 mg | $300.00 | 1 | |
ISRIB enhances the initiation phase of protein synthesis by acting as an integrated stress response (ISR) inhibitor. It can promote the activity of eIF3M by countering the inhibitory effects of stress-induced eIF2α phosphorylation on the initiation complex. | ||||||
Rapamycin | 53123-88-9 | sc-3504 sc-3504A sc-3504B | 1 mg 5 mg 25 mg | $63.00 $158.00 $326.00 | 233 | |
Rapamycin inhibits mTOR, a kinase that regulates protein synthesis. Inhibition of mTOR leads to a downregulation of cap-dependent translation, which may cause a compensatory upregulation in cap-independent translation initiation mechanisms, potentially involving eIF3M. | ||||||
Spermidine | 124-20-9 | sc-215900 sc-215900B sc-215900A | 1 g 25 g 5 g | $57.00 $607.00 $176.00 | ||
Spermidine promotes autophagy, which can lead to the degradation of selective translation repressors and thus indirectly enhances translation initiation. This autophagic response may upregulate the functional activity of eIF3M involved in this process. | ||||||
Tunicamycin | 11089-65-9 | sc-3506A sc-3506 | 5 mg 10 mg | $172.00 $305.00 | 66 | |
Tunicamycin inhibits N-linked glycosylation, causing ER stress and activation of the UPR which can enhance translation initiation to manage misfolded proteins. This stress mechanism may involve upregulation of factors like eIF3M to support increased protein folding demand. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $60.00 $265.00 $1000.00 | 163 | |
MG132 is a proteasome inhibitor that leads to the accumulation of ubiquitinated proteins, invoking cellular stress responses that can enhance translation initiation. The protein eIF3M, being part of the initiation complex, could be functionally activated to address the increased need for protein synthesis. | ||||||
Homoharringtonine | 26833-87-4 | sc-202652 sc-202652A sc-202652B | 1 mg 5 mg 10 mg | $52.00 $125.00 $182.00 | 11 | |
Homoharringtonine inhibits protein synthesis by preventing the initial elongation step of translation. This inhibition can cause a compensatory increase in the need for translation initiation, potentially enhancing the activity of initiation factors such as eIF3M. | ||||||