eIF2C4 Inhibitors
eIF2C4, also recognized as Argonaute-2 (AGO2), is integral to the RNA-induced silencing complex (RISC), facilitating the gene silencing mechanism pivotal for the regulation of gene expression at the post-transcriptional level. This protein binds to microRNAs (miRNAs) and small interfering RNAs (siRNAs), directing the RISC to degrade or inhibit the translation of target mRNAs. The process is vital for various cellular processes, including differentiation, proliferation, and apoptosis, by ensuring the precise expression of genes. eIF2C4's role in gene silencing underscores its importance in maintaining cellular and genetic stability, as well as in the regulation of gene expression in response to physiological and environmental stimuli. The precise mechanism through which eIF2C4 engages with miRNAs or siRNAs and subsequently guides the RISC to its target mRNAs is crucial for the fidelity of gene silencing, highlighting the protein's role in the complex regulatory networks that govern cellular function.
The inhibition of eIF2C4's activity disrupts the RNA interference (RNAi) pathway, affecting the regulation of gene expression crucial for cell survival and function. Inhibition can occur through various mechanisms, including the direct interaction with proteins or small molecules that block the RNA binding domain, blocking eIF2C4 from associating with miRNAs or siRNAs. Additionally, alterations in the phosphorylation status of eIF2C4 can modulate its activity, where specific phosphorylation events might inhibit its incorporation into the RISC or its interaction with RNA molecules. Furthermore, the competitive binding of non-coding RNAs or the sequestration of eIF2C4 by viral proteins can serve as a mechanism of inhibition, effectively blocking eIF2C4 from participating in the RISC assembly. Such inhibitory interactions not only impede the RNAi pathway but also illuminate the complex regulation of eIF2C4, demonstrating how its activity is tightly controlled within the cell to ensure proper gene regulation. These mechanisms of inhibition highlight the intricate balance required for the precise control of gene expression and the consequences of dysregulation within this essential pathway.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
GW4869 | 6823-69-4 | sc-218578 sc-218578A | 5 mg 25 mg | $199.00 $599.00 | 24 | |
GW4869 is an inhibitor of sphingomyelinase, an enzyme involved in lipid metabolism and vesicular trafficking. Inhibition of sphingomyelinase can indirectly affect eIF2C4 function by disrupting lipid pathways that may play a role in RNA interference and microRNA-mediated gene regulation. | ||||||
2′-O-Methyl-5-methylcytidine | 113886-70-7 | sc-283497 sc-283497A | 250 mg 500 mg | $400.00 $650.00 | ||
2'-O-Methyl-5-Methylcytidine is an inhibitor of RNA methyltransferases, which catalyze the methylation of RNA substrates involved in RNA interference. Inhibition of these enzymes can indirectly influence eIF2C4 by altering the methylation status of RNA molecules that participate in the RNA interference process. | ||||||
MLN8237 | 1028486-01-2 | sc-394162 | 5 mg | $220.00 | ||
Alisertib is an Aurora kinase inhibitor that disrupts cell cycle progression by targeting Aurora A kinase. This disruption can indirectly affect eIF2C4 function, as eIF2C4 is implicated in RNA interference and gene regulation processes that intersect with the cell cycle. | ||||||
Staurosporine | 62996-74-1 | sc-3510 sc-3510A sc-3510B | 100 µg 1 mg 5 mg | $82.00 $150.00 $388.00 | 113 | |
Staurosporine is a broad-spectrum protein kinase inhibitor that can modulate various signaling pathways. By altering the phosphorylation state of proteins involved in RNA interference and gene regulation, staurosporine may indirectly impact eIF2C4 function. | ||||||
Trichostatin A | 58880-19-6 | sc-3511 sc-3511A sc-3511B sc-3511C sc-3511D | 1 mg 5 mg 10 mg 25 mg 50 mg | $149.00 $470.00 $620.00 $1199.00 $2090.00 | 33 | |
Trichostatin A is an HDAC inhibitor that modifies gene expression patterns by inhibiting histone deacetylases. Indirectly, it may influence eIF2C4 expression by modifying the acetylation status of histones and chromatin, potentially affecting the accessibility of eIF2C4-regulated genes. | ||||||
Rapamycin | 53123-88-9 | sc-3504 sc-3504A sc-3504B | 1 mg 5 mg 25 mg | $62.00 $155.00 $320.00 | 233 | |
Rapamycin is an mTOR inhibitor that disrupts cellular processes intersecting with eIF2C4 function. By inhibiting mTOR-mediated signaling, rapamycin may indirectly impact eIF2C4 and its role in RNA interference and gene regulation. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin is an ER stress inducer that activates signaling pathways that may indirectly affect eIF2C4. ER stress-induced signaling cascades intersect with eIF2C4-regulated processes, potentially modulating its function in RNA interference and gene regulation. | ||||||
Colchicine | 64-86-8 | sc-203005 sc-203005A sc-203005B sc-203005C sc-203005D sc-203005E | 1 g 5 g 50 g 100 g 500 g 1 kg | $98.00 $315.00 $2244.00 $4396.00 $17850.00 $34068.00 | 3 | |
Colchicine is a microtubule inhibitor that disrupts microtubules involved in cellular processes linked to eIF2C4 indirectly. Disruption of microtubules may impact cellular dynamics and signaling pathways that intersect with eIF2C4-regulated processes, influencing its function in RNA interference and gene regulation. | ||||||
Nifedipine | 21829-25-4 | sc-3589 sc-3589A | 1 g 5 g | $58.00 $170.00 | 15 | |
Nifedipine is an inhibitor of calcium channels, which can modulate intracellular calcium levels. By affecting calcium-dependent processes, these blockers may indirectly influence eIF2C4 function, as eIF2C4 is involved in RNA interference and gene regulation pathways that can be influenced by calcium signaling. | ||||||
2-Deoxy-D-glucose | 154-17-6 | sc-202010 sc-202010A | 1 g 5 g | $65.00 $210.00 | 26 | |
2-Deoxy-D-Glucose (2-DG) is a compound that targets glycolysis and can impact cellular metabolism. Altered cellular metabolism may affect eIF2C4 function indirectly in RNA interference and gene regulation processes influenced by energy availability. | ||||||