DDX35 Inhibitors
Chemical inhibitors of DDX35 act through diverse mechanisms to disrupt its helicase function, which is essential for RNA processing. Ethidium bromide, by intercalating into RNA, can stabilize RNA secondary structures, making it more challenging for DDX35 to bind and unravel the nucleic acid strands. This stabilization of RNA structures by Ethidium bromide can result in the functional inhibition of DDX35. Novobiocin, though primarily targeting DNA gyrase, can also affect the supercoiling of DNA. This altered topology may, in turn, affect the availability or structure of DNA-RNA hybrids, which are potential substrates for DDX35, leading to an indirect inhibition of its activity.
Further, chemical inhibitors like Aurintricarboxylic acid and Distamycin A target nucleic acid interactions, with the former binding to nucleic acids and potentially obstructing DDX35's access to RNA substrates, and the latter binding to the minor groove of DNA, potentially altering the formation of structures such as R-loops, which are substrates for DDX35. 5,6-Dichloro-1-β-D-ribofuranosylbenzimidazole (DRB) inhibits cellular kinases and may thus interfere with the phosphorylation that is crucial for the optimal activity of DDX35. Bisphenol A, by binding nucleic acids, may occupy the binding sites or change the conformation of substrates, thereby inhibiting the helicase activity of DDX35. Ruthenium Red, being a polycationic dye that binds to nucleic acids, can obstruct DDX35's interaction with its substrates, while Plumbagin, through DNA intercalation, can affect the availability of RNA-DNA hybrids for DDX35. Camptothecin stabilizes the DNA-topoisomerase I complex affecting DNA topology which can influence the substrates for DDX35. Lastly, Suramin, a polysulfonated naphthylurea, by binding to nucleic acids and proteins, can prevent DDX35 from accessing its required substrates, thus inhibiting its helicase activity.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Aurintricarboxylic Acid | 4431-00-9 | sc-3525 sc-3525A sc-3525B sc-3525C | 100 mg 1 g 5 g 10 g | $20.00 $32.00 $48.00 $94.00 | 13 | |
Aurintricarboxylic acid inhibits nucleic acid enzymes that interact with nucleic acids. By binding to nucleic acids, it may block the access of DDX35 to its RNA substrates, thus functionally inhibiting its helicase activity. | ||||||
Ethidium bromide | 1239-45-8 | sc-203735 sc-203735A sc-203735B sc-203735C | 1 g 5 g 25 g 100 g | $48.00 $150.00 $588.00 $2086.00 | 12 | |
Ethidium bromide intercalates into nucleic acids, which can inhibit DDX35 by stabilizing RNA secondary structures and making it more difficult for the helicase to bind and unwind RNA. | ||||||
Novobiocin | 303-81-1 | sc-362034 sc-362034A | 5 mg 25 mg | $128.00 $380.00 | ||
Novobiocin is known to target DNA gyrase, but it can also affect DNA topology. By altering the supercoiling of DNA, it can indirectly affect the substrate availability for DDX35, which may interact with DNA-RNA hybrids or R-loops as part of its helicase function. | ||||||
Actinomycin D | 50-76-0 | sc-200906 sc-200906A sc-200906B sc-200906C sc-200906D | 5 mg 25 mg 100 mg 1 g 10 g | $74.00 $243.00 $731.00 $2572.00 $21848.00 | 53 | |
Actinomycin D binds to DNA and by doing so, it can inhibit transcription and also the formation of DNA-RNA hybrids or R-loops, which DDX35 may require as substrates for its helicase activity. | ||||||
DRB | 53-85-0 | sc-200581 sc-200581A sc-200581B sc-200581C | 10 mg 50 mg 100 mg 250 mg | $43.00 $189.00 $316.00 $663.00 | 6 | |
DRB is a kinase inhibitor that can inhibit cellular kinases. DDX35 may require phosphorylation for full activity or interaction with phosphorylated proteins; hence, DRB could reduce the phosphorylation-dependent regulation of DDX35, leading to its functional inhibition. | ||||||
Bisphenol A | 80-05-7 | sc-391751 sc-391751A | 100 mg 10 g | $300.00 $490.00 | 5 | |
Bisphenol A has been shown to bind to nucleic acids, and by this interaction, it can inhibit DDX35 by altering the structure of its RNA substrates or by competing with DDX35 for binding to nucleic acids, thus preventing the helicase from performing its function. | ||||||
Ruthenium red | 11103-72-3 | sc-202328 sc-202328A | 500 mg 1 g | $188.00 $250.00 | 13 | |
Ruthenium Red is a polycationic dye that can bind to nucleic acids and may inhibit DDX35 by blocking its access to RNA substrates or by altering their structure, which is necessary for the helicase's activity. | ||||||
Plumbagin | 481-42-5 | sc-253283 sc-253283A | 100 mg 250 mg | $52.00 $62.00 | 6 | |
Plumbagin has been reported to intercalate into DNA. Through this interaction, it can influence the formation of substrates such as R-loops or RNA-DNA hybrids that DDX35 requires for its helicase function, leading to the functional inhibition of DDX35. | ||||||
Camptothecin | 7689-03-4 | sc-200871 sc-200871A sc-200871B | 50 mg 250 mg 100 mg | $58.00 $186.00 $94.00 | 21 | |
Camptothecin stabilizes the DNA-topoisomerase I complex, which can result in the alteration of DNA topology. Such alterations can indirectly inhibit DDX35 by affecting the structure and availability of DNA-RNA hybrids or R-loops that DDX35 may act upon as part of its helicase function. | ||||||
Suramin sodium | 129-46-4 | sc-507209 sc-507209F sc-507209A sc-507209B sc-507209C sc-507209D sc-507209E | 50 mg 100 mg 250 mg 1 g 10 g 25 g 50 g | $152.00 $214.00 $728.00 $2601.00 $10965.00 $21838.00 $41096.00 | 5 | |
Suramin is a polysulfonated naphthylurea that inhibits various enzymes. It can bind to nucleic acids and proteins, and it may inhibit DDX35 by preventing the protein from interacting with its RNA or DNA substrates, thereby inhibiting the helicase's activity. | ||||||