CENP-N Activators
CENP-N activators encompass a variety of compounds that indirectly enhance the functionality of CENP-N through stabilization of microtubules, modulation of phosphorylation states, and activation of cell cycle checkpoints. Taxol (Paclitaxel), for instance, fortify microtubule polymerization, which is fundamental for the kinetochore function of CENP-N during chromosomeseparation. By ensuring the structural integrity of microtubules, these agents indirectly support the pivotal role of CENP-N in securing kinetochore-microtubule attachments, a critical aspect of accurate chromosome alignment and segregation. Similarly, Calyculin A and Okadaic Acid induce hyperphosphorylation of kinetochore components by inhibiting protein phosphatases. This phosphorylation cascade is likely to enhance the localization and function of CENP-N at the kinetochore, facilitating its role in the dynamic regulation of chromosome movement.
Agents such as Monastrol and S-Trityl-L-cysteine, which disrupt the function of kinesin proteins, lead to spindle checkpoint activation. This heightened checkpoint signaling can, in turn, amplify the recruitment of CENP-N to the kinetochore to maintain genomic stability during cell division. Meanwhile, MG132 prevents proteasomal degradation of cell cycle proteins, potentially increasing the stability and presence of CENP-N at kinetochores. Furthermore, compounds like ZM447439, BI2536, and Hesperadin target specific kinases involved in mitotic progression. By inhibiting Aurora kinases and Plk1, these inhibitors contribute to a checkpoint-induced upregulation of CENP-N activity, ensuring proper kinetochore-microtubule tension and signaling.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Taxol | 33069-62-4 | sc-201439D sc-201439 sc-201439A sc-201439E sc-201439B sc-201439C | 1 mg 5 mg 25 mg 100 mg 250 mg 1 g | $41.00 $74.00 $221.00 $247.00 $738.00 $1220.00 | 39 | |
Taxol stabilizes microtubules and enhances their polymerization, which is crucial during mitosis. CENP-N, being a component of the kinetochore complex, plays a role in chromosome alignment and segregation. Stabilized microtubules could enhance CENP-N's function in kinetochore-microtubule interactions. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A is a potent inhibitor of protein phosphatases 1 and 2A. The inhibition of these phosphatases could increase phosphorylation levels of kinetochore proteins, including CENP-N, potentially enhancing its kinetochore localization and function during cell division. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Similar to Calyculin A, Okadaic Acid inhibits protein phosphatases, leading to increased phosphorylation within the kinetochore. This could indirectly enhance CENP-N's role in chromosome segregation by promoting its interaction with other phosphorylated kinetochore proteins. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $60.00 $265.00 $1000.00 | 163 | |
MG132 is a proteasome inhibitor that can lead to the accumulation of proteins involved in the cell cycle, including those associated with the kinetochore. This could indirectly enhance the stability and function of CENP-N by preventing its degradation during cell division. | ||||||
Monastrol | 254753-54-3 | sc-202710 sc-202710A | 1 mg 5 mg | $120.00 $233.00 | 10 | |
Monastrol is a kinesin-5 inhibitor, leading to the arrest of cells in mitosis with monopolar spindles. This could indirectly enhance CENP-N function by upregulating the spindle assembly checkpoint and increasing CENP-N's role in checkpoint signaling at the kinetochore. | ||||||
S-Trityl-L-cysteine | 2799-07-7 | sc-202799 sc-202799A | 1 g 5 g | $32.00 $66.00 | 6 | |
S-Trityl-L-cysteine is an inhibitor of the mitotic kinesin Eg5. Similar to Monastrol, it could enhance CENP-N function indirectly by activating the spindle assembly checkpoint, which in turn could lead to increased CENP-N engagement in the kinetochore. | ||||||
ZM-447439 | 331771-20-1 | sc-200696 sc-200696A | 1 mg 10 mg | $153.00 $356.00 | 15 | |
ZM447439 is an Aurora kinase inhibitor. By inhibiting Aurora kinases, it could lead to an increase in the spindle assembly checkpoint activity, possibly enhancing the recruitment and function of CENP-N in the kinetochore complex. | ||||||
BI 2536 | 755038-02-9 | sc-364431 sc-364431A | 5 mg 50 mg | $151.00 $525.00 | 8 | |
BI2536 is a Plk1 inhibitor which could lead to the disruption of spindle formation and the activation of the spindle assembly checkpoint, potentially enhancing CENP-N's engagement in kinetochore function during the cell cycle. | ||||||
Hesperadin | 422513-13-1 | sc-490384 | 10 mg | $304.00 | ||
Hesperadin inhibits Aurora B kinase, which could lead to improper kinetochore-microtubule attachments and the activation of the spindle assembly checkpoint. This could increase the recruitment and function of CENP-N at the kinetochore. | ||||||