CCDC158 Activators
Chemical activators of coiled-coil domain containing 158 can engage various signal transduction pathways to modulate the functional state of the protein. Forskolin acts by directly stimulating adenylate cyclase, thereby increasing the levels of cAMP within the cell. This surge in cAMP activates protein kinase A (PKA), which can phosphorylate numerous substrates, including coiled-coil domain containing 158, if it is part of the PKA-responsive pathway. Similarly, dibutyryl-cAMP, a synthetic cAMP analog, permeates the cell membrane and directly activates PKA, again potentially targeting proteins associated with coiled-coil domain containing 158 for phosphorylation. IBMX, by inhibiting the phosphodiesterases responsible for cAMP breakdown, indirectly promotes the same outcome as forskolin and dibutyryl-cAMP by sustaining elevated cAMP levels and thereby prolonging PKA activity.
In addition to cAMP-dependent mechanisms, other pathways converge on the phosphorylation state of proteins like coiled-coil domain containing 158. PMA activates protein kinase C (PKC), which phosphorylates a different set of proteins that can include those in the coiled-coil domain containing 158 pathway. Ionomycin and A23187, both calcium ionophores, increase intracellular calcium concentrations, which can activate calmodulin-dependent kinases capable of targeting proteins in proximity to coiled-coil domain containing 158. Spermine NONOate releases nitric oxide that stimulates soluble guanylyl cyclase, increasing cGMP and activating protein kinase G (PKG), which may also phosphorylate proteins linked to coiled-coil domain containing 158. Furthermore, inhibitors of protein phosphatases like okadaic acid, calyculin A, and cantharidin prevent dephosphorylation of proteins, thereby maintaining coiled-coil domain containing 158 in a phosphorylated, and thus active, state. Anisomycin triggers stress-activated protein kinases, which can further modify the phosphorylation landscape encompassing coiled-coil domain containing 158. Collectively, these chemicals modulate the activity of coiled-coil domain containing 158 by altering the balance of phosphorylation and dephosphorylation within the cell.
SEE ALSO...
Items 1 to 10 of 12 total
Display:
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylate cyclase, increasing intracellular cAMP levels, which can lead to the activation of protein kinase A (PKA). PKA can phosphorylate and thereby activate proteins within the same signaling pathway as coiled-coil domain containing 158, leading to its functional activation. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
Isobutylmethylxanthine (IBMX) is a nonspecific inhibitor of phosphodiesterases, enzymes responsible for the breakdown of cAMP. By preventing cAMP degradation, IBMX can enhance the signaling through the cAMP/PKA pathway, which can activate proteins associated with the same pathway as coiled-coil domain containing 158. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
Phorbol 12-myristate 13-acetate (PMA) is an activator of protein kinase C (PKC). PKC activation can lead to phosphorylation cascades that activate proteins within related signaling pathways, potentially leading to the functional activation of coiled-coil domain containing 158. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Elevated calcium can activate calmodulin-dependent kinase (CaMK), which may phosphorylate and activate proteins within the same pathway as coiled-coil domain containing 158. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 is another calcium ionophore that raises intracellular calcium levels, similarly activating CaMK and potentially leading to the activation of proteins within the same pathway as coiled-coil domain containing 158. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
Epigallocatechin gallate (EGCG) can influence signaling pathways by inhibiting protein tyrosine kinases, which may result in an altered phosphorylation state of proteins associated with coiled-coil domain containing 158, leading to its activation. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable cAMP analog that activates PKA. Activated PKA can phosphorylate proteins within the same signaling pathways as coiled-coil domain containing 158, potentially leading to its activation. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases 1 and 2A, leading to increased phosphorylation levels of proteins within the pathways that include coiled-coil domain containing 158, potentially activating it. | ||||||
Spermine NONOate | 136587-13-8 | sc-202816 sc-202816A | 5 mg 25 mg | $52.00 $192.00 | 5 | |
Spermine NONOate donates nitric oxide, which can activate soluble guanylyl cyclase, increasing cGMP levels. This can activate protein kinase G (PKG), which might phosphorylate and activate proteins associated with coiled-coil domain containing 158. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that can activate stress-activated protein kinases (SAPKs), such as JNK. Activation of these kinases can lead to phosphorylation cascades that involve proteins associated with coiled-coil domain containing 158, potentially activating it. | ||||||