C12orf23 Activators
Chemical activators of C12orf23 can instigate a series of intracellular signaling events that lead to the protein's activation through phosphorylation. Forskolin elevates intracellular cAMP levels, thereby activating protein kinase A (PKA). This activation cascade can result in the phosphorylation of C12orf23 if it serves as a substrate for PKA. Similarly, isoproterenol, functioning as a beta-adrenergic agonist, also raises cAMP levels and activates PKA, suggesting a parallel route to C12orf23 activation. Dibutyryl cyclic AMP (db-cAMP), which is a cAMP analog, can directly stimulate PKA without the need for upstream cAMP generation, providing a more direct means for C12orf23 phosphorylation and activation. Phorbol 12-myristate 13-acetate (PMA) influences cellular processes by activating protein kinase C (PKC), which in turn may phosphorylate C12orf23 if it is recognized by PKC as a substrate.
Furthermore, the intracellular calcium concentration is a crucial factor in the activation of various calcium-dependent kinases capable of phosphorylating C12orf23. Ionomycin and A23187 (Calcimycin) both act as calcium ionophores, increasing the intracellular calcium concentration, and potentially leading to the activation of C12orf23 through calcium-sensitive kinases. Calcium levels can also be influenced by Zinc Pyrithione, which increases intracellular zinc that can serve as a cofactor for kinase enzymes that phosphorylate C12orf23. Epidermal Growth Factor (EGF) triggers its receptor EGFR, setting off a series of phosphorylation events within the MAPK/ERK and PI3K pathways, which could include the activation of C12orf23. Insulin, through the PI3K/Akt signaling pathway, and spermidine, which may activate AMP-activated protein kinase (AMPK), provide additional avenues for the activation of C12orf23 through phosphorylation by these kinases. Lastly, 5-Azacytidine can impact kinase signaling pathways, which may alter the phosphorylation state and activity of C12orf23.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin acts as a calcium ionophore, raising intracellular calcium levels. Increased calcium can activate calcium-dependent protein kinases, such as CaMKII, which could phosphorylate C12orf23 if it is recognized as a substrate, leading to its activation. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC), which phosphorylates a variety of substrates. If C12orf23 is a PKC substrate, its activation by PMA would occur through phosphorylation by PKC. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187, like ionomycin, increases intracellular calcium concentrations, potentially activating calcium-sensitive kinases that could phosphorylate and activate C12orf23. | ||||||
Retinoic Acid, all trans | 302-79-4 | sc-200898 sc-200898A sc-200898B sc-200898C | 500 mg 5 g 10 g 100 g | $65.00 $319.00 $575.00 $998.00 | 28 | |
Retinoic acid can influence cellular differentiation pathways, which may include kinase signaling cascades. If C12orf23 is part of a pathway influenced by retinoic acid, kinases activated as a result could phosphorylate and activate C12orf23. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $153.00 $1224.00 $12239.00 | 82 | |
Insulin activates the PI3K/Akt signaling pathway, which regulates a host of cellular functions including protein phosphorylation. If C12orf23 is a downstream target in this pathway, its activation could be mediated by Akt or other kinases activated by insulin. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $47.00 | ||
Zinc Pyrithione can increase intracellular zinc levels, which can act as a cofactor for various enzymes including protein kinases. If C12orf23 activity is contingent on phosphorylation by a zinc-dependent kinase, its activation could be mediated by such a mechanism. | ||||||
Spermidine | 124-20-9 | sc-215900 sc-215900B sc-215900A | 1 g 25 g 5 g | $56.00 $595.00 $173.00 | ||
Spermidine can induce autophagy and may activate AMP-activated protein kinase (AMPK). If C12orf23 is involved in autophagy processes or is regulated by AMPK, spermidine could lead to its activation. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $27.00 $37.00 | 5 | |
Isoproterenol is a beta-adrenergic agonist that increases cAMP levels in cells, leading to the activation of PKA. If C12orf23 is a substrate for PKA, its activation could follow PKA activation by isoproterenol. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
db-cAMP is a cell-permeable cAMP analog that directly activates PKA. Subsequent phosphorylation by PKA could activate C12orf23 if it is a substrate for this kinase. | ||||||
5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
While primarily known for its DNA methyltransferase inhibitory activity, 5-Azacytidine can also influence kinase signaling pathways. Assuming C12orf23 is regulated by a kinase pathway susceptible to modulation by cellular methylation patterns, 5-Azacytidine could lead to the activation of C12orf23 through downstream effects on kinases. | ||||||