Santa Cruz Biotechnology offers Phosphatase Inhibitor Cocktails to preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction. Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material. Phosphatase Inhibitor Cocktail A and C inhibits alkaline phosphatase as well as serine/threonine protein phosphatases. Phosphatase Inhibitor Cocktail B inhibits tyrosine protein phosphatases, acid and alkaline phosphatases.
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