9330182L06Rik Inhibitors
Chemical inhibitors of endosome-lysosome associated apoptosis and autophagy regulator family member 2 (ELAAFR2) operate through various modes to disrupt the protein's function. Chloroquine and hydroxychloroquine, for example, accumulate in the acidic vesicles of endosomes and lysosomes, leading to an increase in pH. This elevated pH can inhibit the activity of enzymes within these organelles that are essential for the operation of ELAAFR2. Similarly, bafilomycin A1 and concanamycin A target the V-ATPase, a critical component responsible for the acidification of lysosomes. By inhibiting V-ATPase, these chemicals contribute to an increased lysosomal pH, which can interfere with the optimal acidic conditions required for ELAAFR2's activity. Monensin and nigericin, as ionophores, disrupt the proton gradient across lysosomal membranes, further contributing to a disturbed pH homeostasis. This disruption can inhibit the function of ELAAFR2 by neutralizing the lysosomal acidic environment, which is crucial for its activity.
On the other hand, E64d and leupeptin act as inhibitors of cysteine proteases and serine proteases, respectively. These proteases are active within lysosomes and are thought to be involved in the proteolytic processes on which ELAAFR2 relies for its function. The inhibition of these enzymes can, therefore, inhibit the functional activity of ELAAFR2. Pepstatin A, by inhibiting aspartyl proteases, and Cathepsin Inhibitor I, by targeting cathepsins, similarly can lead to the inhibition of the proteolytic activation of enzymes that ELAAFR2 requires. Additionally, Z-VAD-FMK, a pan-caspase inhibitor, can inhibit the function of caspases within the apoptosis pathway, which could indirectly affect the regulatory role of ELAAFR2 in apoptosis. Finally, 3-Methyladenine inhibits phosphoinositide 3-kinases, which are involved in autophagy induction, a process in which ELAAFR2 is implicated. Inhibition of these kinases can inhibit autophagy, thereby influencing the functional activity of ELAAFR2 associated with this process.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Chloroquine | 54-05-7 | sc-507304 | 250 mg | $68.00 | 2 | |
Chloroquine accumulates in the acidic vesicles of endosomes and lysosomes, leading to an increase in pH. The increased pH can inhibit the activity of enzymes within these organelles, potentially inhibiting the function of endosome-lysosome associated apoptosis and autophagy regulator family member 2, which relies on the acidic environment for its role in apoptosis and autophagy. | ||||||
hydroxychloroquine | 118-42-3 | sc-507426 | 5 g | $56.00 | 1 | |
Similar to chloroquine, hydroxychloroquine raises the pH in endosomes and lysosomes, which can inhibit the enzymatic activities necessary for the function of endosome-lysosome associated apoptosis and autophagy regulator family member 2 by disrupting the organelle's environment. | ||||||
Bafilomycin A1 | 88899-55-2 | sc-201550 sc-201550A sc-201550B sc-201550C | 100 µg 1 mg 5 mg 10 mg | $96.00 $250.00 $750.00 $1428.00 | 280 | |
This chemical inhibits V-ATPase, which is responsible for acidifying lysosomes. Inhibition of V-ATPase will increase lysosomal pH, which could inhibit the function of endosome-lysosome associated apoptosis and autophagy regulator family member 2 by altering the lysosomal environment where the protein is active. | ||||||
Concanamycin A | 80890-47-7 | sc-202111 sc-202111A sc-202111B sc-202111C | 50 µg 200 µg 1 mg 5 mg | $65.00 $162.00 $650.00 $2550.00 | 109 | |
Concanamycin A is another inhibitor of V-ATPase, which leads to an increased lysosomal pH. This change in pH can inhibit the function of endosome-lysosome associated apoptosis and autophagy regulator family member 2 by impairing the protein's optimal acidic conditions for activity. | ||||||
Monensin A | 17090-79-8 | sc-362032 sc-362032A | 5 mg 25 mg | $152.00 $515.00 | ||
Monensin acts as an ionophore that disrupts the proton gradient across lysosomal membranes. By disturbing the pH homeostasis, it could inhibit the function of endosome-lysosome associated apoptosis and autophagy regulator family member 2, which requires the acidic environment for its activity. | ||||||
Nigericin sodium salt | 28643-80-3 | sc-201518A sc-201518 sc-201518B sc-201518C sc-201518D | 1 mg 5 mg 25 mg 1 g 5 g | $45.00 $110.00 $235.00 $6940.00 $26879.00 | 9 | |
Nigericin, a potassium ionophore, disrupts lysosomal pH by exchanging H+ ions for K+ ions across the membrane, which could inhibit the function of endosome-lysosome associated apoptosis and autophagy regulator family member 2 by neutralizing the lysosomal acidic environment. | ||||||
Calpeptin | 117591-20-5 | sc-202516 sc-202516A | 10 mg 50 mg | $119.00 $447.00 | 28 | |
This chemical inhibits cathepsins, which are lysosomal hydrolases. Inhibiting cathepsins can lead to the functional inhibition of endosome-lysosome associated apoptosis and autophagy regulator family member 2, which may depend on cathepsins for processing or activation. | ||||||
Z-VAD-FMK | 187389-52-2 | sc-3067 | 500 µg | $74.00 | 256 | |
Z-VAD-FMK is a pan-caspase inhibitor that can inhibit the function of caspases within the apoptosis pathway. By inhibiting these caspases, it could indirectly inhibit the function of endosome-lysosome associated apoptosis and autophagy regulator family member 2, which is implicated in apoptosis regulation. | ||||||
Leupeptin hemisulfate | 103476-89-7 | sc-295358 sc-295358A sc-295358D sc-295358E sc-295358B sc-295358C | 5 mg 25 mg 50 mg 100 mg 500 mg 10 mg | $72.00 $145.00 $265.00 $489.00 $1399.00 $99.00 | 19 | |
Leupeptin inhibits serine and cysteine proteases within lysosomes. Inhibition of these enzymes could indirectly inhibit the function of endosome-lysosome associated apoptosis and autophagy regulator family member 2 by preventing the proteolytic processes it may depend on. | ||||||
Autophagy Inhibitor, 3-MA | 5142-23-4 | sc-205596 sc-205596A | 50 mg 500 mg | $56.00 $256.00 | 113 | |
3-Methyladenine is known to inhibit phosphoinositide 3-kinases, which play a role in autophagy induction. Inhibition of these kinases can inhibit autophagy, potentially affecting the function of endosome-lysosome associated apoptosis and autophagy regulator family member 2 that is involved in the autophagy process. | ||||||