4921511H03Rik Activators
Chemical activators of testis expressed 47 can engage various cellular signaling pathways to modulate the protein's activity through phosphorylation. Forskolin, by elevating intracellular levels of cAMP, can activate protein kinase A (PKA), which may in turn phosphorylate testis expressed 47, altering its activity. Similarly, dibutyryl-cAMP, a membrane-permeable analog of cAMP, can also activate PKA and elicit the same effect on testis expressed 47. Ionomycin, through its role as a calcium ionophore, raises the intracellular concentration of calcium, which could activate calcium/calmodulin-dependent kinases capable of phosphorylating testis expressed 47. This process potentially engages testis expressed 47 in calcium-sensitive signaling pathways.
Further activation routes involve protein kinase C (PKC), which can be activated by phorbol 12-myristate 13-acetate (PMA) and, under certain conditions, by Bisindolylmaleimide I. PKC, once activated, could directly phosphorylate testis expressed 47 if it is a PKC substrate. The physiological response to insulin involves the activation of the PI3K/Akt pathway, where Akt kinase can phosphorylate a broad range of proteins, including possibly testis expressed 47. Similarly, the engagement of the epidermal growth factor (EGF) with its receptor can initiate the MAPK/ERK pathway, which might result in the phosphorylation and subsequent activation of testis expressed 47. Anisomycin, a known activator of MAPKs like JNK and p38, could also contribute to the phosphorylation state of testis expressed 47. Additionally, the inhibition of protein phosphatases PP1 and PP2A by compounds such as Okadaic Acid and Calyculin A may lead to the sustained phosphorylation and consequential activation of testis expressed 47. Lastly, the presence of zinc pyrithione can increase intracellular zinc levels, which may alter kinase and phosphatase activity, possibly resulting in the phosphorylation of testis expressed 47.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin acts as an ionophore for calcium, increasing intracellular calcium concentrations. This can activate Ca²⁺/calmodulin-dependent protein kinases, which may phosphorylate and activate testis expressed 47 as part of a signaling cascade that involves calcium-sensitive pathways. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which can phosphorylate a variety of proteins. PKC-mediated phosphorylation can directly activate testis expressed 47 if it is a substrate of PKC or if PKC phosphorylation activates a kinase cascade that includes testis expressed 47. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $156.00 $1248.00 $12508.00 | 82 | |
Insulin binding to its receptor triggers the PI3K/Akt signaling pathway. Akt can phosphorylate a multitude of proteins, potentially including testis expressed 47, which would result in its activation as part of the cell's response to insulin. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is known to activate MAPKs, such as JNK and p38 MAPK. These kinases can phosphorylate a range of substrates, potentially including testis expressed 47, which would result in the activation of the protein as part of the cellular stress response. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $105.00 $242.00 | 36 | |
Bisindolylmaleimide I, while typically a PKC inhibitor, under certain conditions can activate specific PKC isoforms. If testis expressed 47 is a substrate for one of these isoforms, this could lead to its direct phosphorylation and activation. | ||||||
(±)-S-Nitroso-N-acetylpenicillamine | 79032-48-7 | sc-200319B sc-200319 sc-200319A | 10 mg 20 mg 100 mg | $74.00 $114.00 $374.00 | 18 | |
SNAP is a nitric oxide donor which can activate soluble guanylyl cyclase, leading to increased levels of cyclic GMP (cGMP). cGMP may activate PKG (protein kinase G), which in turn could phosphorylate and activate testis expressed 47, provided that testis expressed 47 is a PKG substrate or part of a pathway regulated by PKG. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid is an inhibitor of protein phosphatases PP1 and PP2A. By inhibiting dephosphorylation, it may result in a sustained phosphorylated state of proteins, including potentially testis expressed 47, leading to its activation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Similar to Okadaic Acid, Calyculin A also inhibits protein phosphatases such as PP1 and PP2A. This inhibition can lead to prolonged phosphorylation and thus activation of testis expressed 47 if it is regulated by these phosphatases. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $48.00 | ||
Zinc Pyrithione can act to increase intracellular zinc levels, which can modulate a variety of signaling pathways. Zinc can activate certain protein kinases or inhibit phosphatases, potentially leading to the phosphorylation and activation of testis expressed 47 if it is sensitive to changes in zinc-mediated signaling. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP is a cAMP analog that can readily enter cells and mimic the action of endogenous cAMP by activating PKA. PKA can then phosphorylate various proteins, potentially including testis expressed 47, leading to its activation within cAMP-dependent signaling pathways. | ||||||