WNK3 Antibody (A-6): sc-515570
- WNK3 Antibody (A-6) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 1-146 mapping at the N-terminus of WNK3 of human origin
- WNK3 Antibody (A-6) is recommended for detection of WNK3 of human origin by WB, IP, IF and ELISA
- Anti-WNK3 Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for WNK3 Antibody (A-6) for WB applications. These reagents are now offered in bundles with WNK3 Antibody (A-6) (see ordering information below).
WNK3 Antibody (A-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects WNK3 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WNK3 Antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WNK3, a member of the WNK kinase family, plays a crucial role in regulating ion transport and blood pressure by modulating the activity of various ion channels and transporters, particularly in the kidney and brain. This regulation is vital for maintaining electrolyte balance and blood pressure homeostasis, and dysregulation of WNK3 has been linked to conditions such as hypertension and renal disorders. WNK3 is primarily expressed in the brain, where WNK3 influences neuronal signaling and function, and aberrant WNK3 activity can lead to significant physiological consequences. The WNK kinase family, which includes WNK1, WNK2, WNK3, and WNK4, is characterized by a unique structure that allows for specific interactions with ATP, essential for their kinase activity. The catalytic domains of WNK2, WNK3, and WNK4 share a high degree of homology with WNK1, underscoring the evolutionary conservation of their functions. Understanding WNK3 and its interactions within cellular signaling pathways is critical for developing targeted therapies for related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
WNK3 Antibody (A-6) References:
- WNK1, a novel mammalian serine/threonine protein kinase lacking the catalytic lysine in subdomain II. | Xu, B., et al. 2000. J Biol Chem. 275: 16795-801. PMID: 10828064
- Human hypertension caused by mutations in WNK kinases. | Wilson, FH., et al. 2001. Science. 293: 1107-12. PMID: 11498583
- WNK kinases, a novel protein kinase subfamily in multi-cellular organisms. | Veríssimo, F. and Jordan, P. 2001. Oncogene. 20: 5562-9. PMID: 11571656
- Human hypertension caused by mutations in WNK kinases. | Hollenberg, NK. 2002. Curr Hypertens Rep. 4: 267. PMID: 12166403
- Regulation of WNK1 by an autoinhibitory domain and autophosphorylation. | Xu, BE., et al. 2002. J Biol Chem. 277: 48456-62. PMID: 12374799
- Compilation and characterization of a novel WNK family of protein kinases in Arabiodpsis thaliana with reference to circadian rhythms. | Nakamichi, N., et al. 2002. Biosci Biotechnol Biochem. 66: 2429-36. PMID: 12506983
- WNK kinases regulate thiazide-sensitive Na-Cl cotransport. | Yang, CL., et al. 2003. J Clin Invest. 111: 1039-45. PMID: 12671053
- Association of WNK1 gene polymorphisms and haplotypes with ambulatory blood pressure in the general population. | Tobin, MD., et al. 2005. Circulation. 112: 3423-9. PMID: 16301342
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WNK3 Antibody (A-6) | sc-515570 | 200 µg/ml | $322.00 | |||
WNK3 Antibody (A-6): m-IgG Fc BP-HRP Bundle | sc-531421 | 200 µg Ab; 10 µg BP | $361.00 | |||
WNK3 Antibody (A-6): m-IgGκ BP-HRP Bundle | sc-525107 | 200 µg Ab, 40 µg BP | $361.00 | |||
WNK3 Antibody (A-6): m-IgG2a BP-HRP Bundle | sc-547786 | 200 µg Ab; 10 µg BP | $361.00 | |||
WNK3 Antibody (A-6) AC | sc-515570 AC | 500 µg/ml, 25% agarose | $424.00 | |||
WNK3 Antibody (A-6) HRP | sc-515570 HRP | 200 µg/ml | $322.00 | |||
WNK3 Antibody (A-6) FITC | sc-515570 FITC | 200 µg/ml | $336.00 | |||
WNK3 Antibody (A-6) PE | sc-515570 PE | 200 µg/ml | $349.00 | |||
WNK3 Antibody (A-6) Alexa Fluor® 488 | sc-515570 AF488 | 200 µg/ml | $364.00 | |||
WNK3 Antibody (A-6) Alexa Fluor® 546 | sc-515570 AF546 | 200 µg/ml | $364.00 | |||
WNK3 Antibody (A-6) Alexa Fluor® 594 | sc-515570 AF594 | 200 µg/ml | $364.00 | |||
WNK3 Antibody (A-6) Alexa Fluor® 647 | sc-515570 AF647 | 200 µg/ml | $364.00 | |||
WNK3 Antibody (A-6) Alexa Fluor® 680 | sc-515570 AF680 | 200 µg/ml | $364.00 | |||
WNK3 Antibody (A-6) Alexa Fluor® 790 | sc-515570 AF790 | 200 µg/ml | $364.00 |