VPS25抗体 (B-4) 推荐用于 mouse, rat 和 human 来源的VPS25 WB, IP, IF, IHC(P) 和 ELISA检测

VPS25 抗体 (B-4): sc-271648

VPS25抗体 (B-4) is rated 5.0 out of 5 by 6.
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说明书

    • 抗-VPS25 抗体 B-4 是小鼠单克隆 IgG1 κ,VPS25抗体, 在5篇文献中引用,规格为200 µg/ml
    • 免疫human物种的代表全长VPS25的氨基酸1-176
    • 抗-VPS25 抗体 (B-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 VPS25
    • 抗-VPS25 抗体 (B-4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取VPS25 (B-4): sc-271648免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • 对于VPS25 抗体 (B-4)的WB and IHC(P)应用,m-IgGκ BP-HRP 是首选的二抗检测试剂。现在该试剂和VPS25 抗体 (B-4)组合成套装提供(参见下面的订购信息)。对于其他m-IgGκ BP 偶联产品,参见我们小鼠IgG 结合蛋白的完整列表
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I am using VPS25 (B-4): sc-271648 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked well for Western Blot. I used this anti-VPS25 for a Western Blot, with m-IgGκ BP-HRP, and it showed specific bands for Vps25 in HeLa whole cell lysates and post-nuclear supernatant fractions.
Date published: 2017-11-30
Rated 5 out of 5 by from Good antibody I used this antibody recently. It works well and less background.
Date published: 2017-09-12
Rated 5 out of 5 by from Reliable antibody Detects a consistent and strong band at the correct molecular weight of ~21 kDa in Western Blot.
Date published: 2017-02-07
Rated 5 out of 5 by from Robust signal in WB in NIH/3T3 whole cell Robust signal in WB in NIH/3T3 whole cell lysate and HeLa nuclear extract. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by from WB publishable data with human HuH WB publishable data with human HuH-7 - SCBT Publication Review
Date published: 2015-03-23
Rated 5 out of 5 by from Produced beautiful immunoperoxidase nuclear Produced beautiful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human nasopharynx tissue. -SCBT QC
Date published: 2013-12-11
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