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V5-Probe Antibody (E10) - Western Blotting - Image 130766
V5-Probe 抗体 (E10): sc-81594. 未转染: sc-117752 (A) 和转染: sc-173792 (B) 293T 全细胞裂解液中 karyopherin α6 表达的 Western 印迹分析.
V5-Probe Antibody (E10) - Western Blotting - Image 130766
V5-Probe 抗体 (E10): sc-81594. 未转染: sc-117752 (A) 和转染: sc-173792 (B) 293T 全细胞裂解液中 karyopherin α6 表达的 Western 印迹分析.
V5-Probe 抗体 (E10): sc-81594. 未转染: sc-117752 (A) 和转染: sc-173792 (B) 293T 全细胞裂解液中 karyopherin α6 表达的 Western 印迹分析.

V5-Probe 抗体 (E10): sc-81594

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说明书
  • V5-Probe 抗体 E10 是小鼠单克隆 IgG1, 在34篇文献中引用，提供 100 µg/ml
  • 针对V5融合蛋白
  • 推荐用于 V5 fusion proteins WB, IP, IF 和 IHC(P)检测
  • 看 V5-Probe (H-9): sc-271926，可查看有标记的 V5-Probe 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是V5-Probe Antibody (E10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与V5-Probe Antibody (E10) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

V5-Probe抗体（E10）是一种IgG1 κ小鼠单克隆V5-Probe抗体（也称为V5-Probe抗体或V5标签抗体），可通过WB、IP、IF和IHC(P)检测V5融合蛋白。V5-Probe抗体（E10）为非偶联的抗V5-Probe抗体形式。质粒载体用于在细菌、昆虫和哺乳动物宿主中表达真核基因的编码区是常见的用法；这种表达载体经常用于编码杂交融合蛋白，该融合蛋白由真核靶蛋白和一个专门设计的区域组成，有助于靶蛋白的纯化和可视化。例如，V5-Probe可识别副粘病毒猴病毒5（SV5）的P/V蛋白上的一个小表位Pk。这个小肽已被证明在可视化和免疫亲和纯化表达的融合蛋白方面非常有用。已有20多种重组蛋白（其中一些包括跨膜蛋白和分泌蛋白）被该表位标记，并通过蛋白质印迹、免疫沉淀和免疫荧光进行检测。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

V5-Probe 抗体 (E10) 参考文献:

  1. 猿猴病毒 5 的 V 蛋白中的单个氨基酸替换可区分其在人类和鼠类细胞中阻断干扰素信号传导的能力。  |  Young, DF., et al. 2001. J Virol. 75: 3363-70. PMID: 11238862
  2. 副粘病毒SV5 V蛋白结合两个锌原子，是病毒颗粒的结构组成部分。  |  Paterson, RG., et al. 1995. Virology. 208: 121-31. PMID: 11831692
  3. 常氧成纤维细胞中单链 DNA 结合冷休克结构域（Y-box）蛋白抑制血管内皮生长因子启动子的新机制  |  Coles, LS., et al. 2002. Nucleic Acids Res. 30: 4845-54. PMID: 12433987
  4. 副粘病毒 SV5 和 HPIV2 利用细胞成分组装 STAT 蛋白泛素连接酶复合物。  |  Ulane, CM. and Horvath, CM. 2002. Virology. 304: 160-6. PMID: 12504558
  5. ERK2和GSK3beta对冷休克结构域/Y-盒蛋白的磷酸化以及对人类血管内皮生长因子启动子的抑制。  |  Coles, LS., et al. 2005. FEBS Lett. 579: 5372-8. PMID: 16198352
  6. 在人类树突状细胞中，猿病毒5（SV5）P/V突变体的细胞病变程度低于野生型SV5，是树突状细胞成熟和功能的更有效激活剂。  |  Arimilli, S., et al. 2006. J Virol. 80: 3416-27. PMID: 16537609
  7. 单步纯化在大肠杆菌中表达的与谷胱甘肽 S-转移酶融合的多肽。  |  Smith, DB. and Johnson, KS. 1988. Gene. 67: 31-40. PMID: 3047011
  8. 大肠杆菌载体，通过与麦芽糖结合蛋白融合和分离，表达和纯化外来蛋白。  |  Maina, CV., et al. 1988. Gene. 74: 365-73. PMID: 3073105
  9. 开发V5标签导向纳米抗体并将其作为GPCR信号的细胞内生物传感器。  |  Zeghal, M., et al. 2023. J Biol Chem. 299: 105107. PMID: 37517699
  10. 大肠杆菌 K12 的 malE 基因及其产物麦芽糖结合蛋白的序列。  |  Duplay, P., et al. 1984. J Biol Chem. 259: 10606-13. PMID: 6088507
  11. Sp1和Egr-1对大鼠苯乙醇胺N-甲基转移酶基因的不同激活作用  |  Ebert, SN. and Wong, DL. 1995. J Biol Chem. 270: 17299-305. PMID: 7615530
  12. 副粘病毒SV5 V和P蛋白的RNA结合区域。  |  Lin, GY., et al. 1997. Virology. 238: 460-9. PMID: 9400618

订购信息

产品名称产品编号规格价格数量收藏夹

V5-Probe 抗体 (E10)

sc-81594
100 µg/ml
$316.00

V5-Probe (E10): m-IgG Fc BP-HRP 套装

sc-537250
100 µg Ab; 10 µg BP
$354.00

V5-Probe (E10): m-IgGκ BP-HRP 套装

sc-534475
100 µg Ab; 40 µg BP
$354.00

V5-Probe (E10): m-IgG1 BP-HRP 套装

sc-545106
100 µg Ab; 20 µg BP
$354.00

At what molecular weight should the band be seen in Western Blot with this antibody?

Asked by: AbPolly
Thank you for your question. The molecular weight expected varies depending on the fusion protein you are trying to detect, equal to the protein of interest plus the weight of the V5 tag that it is fused to.
Answered by: Technical Support 8
Date published: 2019-06-06
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Rated 3 out of 5 by from blots ok but cross-reacting band problemsSpent a lot of time trying to sort out the specificity of this antibody. We tried to use to look at cell-cell junctions with V5-tagged proteins but it recognized junctions directly (no V5 tagged protein necessary), especially at higher concentrations. In the end we could not use any of the data. On westerns it works ok but has a cross-reacting band around 150K that we wonder might be a junctional protein.
Date published: 2023-12-05
Rated 3 out of 5 by from Antibody reviewWorked with WB at 1:500 dilution with mosue tissue sample.
Date published: 2018-05-29
Rated 4 out of 5 by from good for western blotThe V5-probe (E10) antibody works nicely for western blot. Havenot tried it in immunofluorescent staining.
Date published: 2018-01-26
Rated 5 out of 5 by from Good antibody for WBDetect V5-tagged protein in HeLa cells, used Ab in 1:1000, very strong and clean band.
Date published: 2018-01-26
Rated 4 out of 5 by from Clean antibodyI received a sample from SCBT and have tested this antibody for western blotting. There is a non-specific band at ~150kDa. Other than that, it is a clean antibody.
Date published: 2017-10-23
Rated 5 out of 5 by from V5-CD40 fusionI have used this twice, and both times it was exceptionally clean, just a single strong band using chemiluminescence.
Date published: 2017-09-15
Rated 5 out of 5 by from clear signalthe antibody was used of human, clear background.very good
Date published: 2017-05-02
Rated 5 out of 5 by from Great monoclonal antibodySpecific signal observed with V5-Probe Antibody (E10)
Date published: 2017-01-18
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V5-Probe 抗体 (E10) is rated 4.2 out of 5 by 12.
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