Date published: 2025-9-10

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V5-Probe 抗体 (C-9): sc-271944

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说明书
  • V5-Probe抗体(C-9)是小鼠单克隆IgG2a κ, 在30篇文献中引用,规格为200 µg/ml
  • 特定于Simian Virus 5起源的the P and V proteins的C-terminal序列内氨基酸87-111之间的表位图谱
  • V5-Probe 抗体 (C-9) 推荐用于 WB, IP, IF 和 ELISA,检测 V5 fusion proteins
  • 抗V5-Probe抗体(C-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取V5-Probe (C-9): sc-271944免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是V5-Probe Antibody (C-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与V5-Probe Antibody (C-9) 打包提供(请参阅下面的订购信息)。

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関連項目

V5-Probe抗体(C-9)是一种IgG2a κ小鼠单克隆V5-Probe抗体(也称为V5-Probe抗体或V5标签抗体),可通过WB、IP、IF和ELISA检测V5融合蛋白。V5-Probe抗体(C-9)既可以是非偶联的抗V5-Probe抗体形式,也可以是多种偶联形式的抗V5-Probe抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。质粒载体用于在细菌、昆虫和哺乳动物宿主中表达真核基因的编码区是常见的用法;这种表达载体经常用于编码杂交融合蛋白,该融合蛋白由真核靶蛋白和一个专门设计的区域组成,有助于靶蛋白的纯化和可视化。例如,V5-Probe可识别副粘病毒猴病毒5(SV5)的P/V蛋白上的一个小表位Pk。这个小肽已被证明在可视化和免疫亲和纯化表达的融合蛋白方面非常有用。已有20多种重组蛋白(其中一些包括跨膜蛋白和分泌蛋白)被该表位标记,并通过蛋白质印迹、免疫沉淀和免疫荧光进行检测。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

V5-Probe 抗体 (C-9)

sc-271944
200 µg/ml
$316.00

V5-Probe (C-9): m-IgG Fc BP-HRP 套装

sc-525839
200 µg Ab; 10 µg BP
$354.00

V5-Probe (C-9): m-IgG2a BP-HRP 套装

sc-546435
200 µg Ab; 10 µg BP
$354.00

V5-Probe 抗体 (C-9) AC

sc-271944 AC
500 µg/ml, 25% agarose
$416.00

V5-Probe 抗体 (C-9) HRP

sc-271944 HRP
200 µg/ml
$316.00

V5-Probe 抗体 (C-9) FITC

sc-271944 FITC
200 µg/ml
$330.00

V5-Probe 抗体 (C-9) PE

sc-271944 PE
200 µg/ml
$343.00

V5-Probe 抗体 (C-9) Alexa Fluor® 488

sc-271944 AF488
200 µg/ml
$357.00

V5-Probe 抗体 (C-9) Alexa Fluor® 546

sc-271944 AF546
200 µg/ml
$357.00

V5-Probe 抗体 (C-9) Alexa Fluor® 594

sc-271944 AF594
200 µg/ml
$357.00

V5-Probe 抗体 (C-9) Alexa Fluor® 647

sc-271944 AF647
200 µg/ml
$357.00

V5-Probe 抗体 (C-9) Alexa Fluor® 680

sc-271944 AF680
200 µg/ml
$357.00

V5-Probe 抗体 (C-9) Alexa Fluor® 790

sc-271944 AF790
200 µg/ml
$357.00

V5-Probe (C-9) 中和勝肽

sc-271944 P
100 µg/0.5 ml
$68.00

is this antibody compatible with ChiP? The discontinued product was, but ChiP is NOT listed and application here and that it what I need it for.

Asked by: MonicaN
Thank you for your question. This mouse monoclonal antibody does not yet have data or product citations using it for ChIP, so it is not a recommended application at this time. We would recommend one of our alternative antibodies, V5-Probe Antibody (E10): sc-81594 , which has 2 known product citations using the antibody for ChIP. These references are on PubMed, PMID's: 29249655 and 20855289.
Answered by: Technical Support
Date published: 2020-03-26

Hello, I would like to know if the "GKPIPNPLLGLDST" is the immunogen used in the production of this antibody? Thanks in advance

Asked by: GUIGO31
Thank you for your question. The exact immunogen sequence is considered proprietary. Please contact Technical Service or your local distributor for information further information.
Answered by: Tech Service
Date published: 2019-03-20

What is the best secondary to use with V5-Probe Antibody (C-9): sc-271944 in Western Blot?

Asked by: cjMara
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 5 out of 5 by from Great Antibody for WBVery clear, specific and strong signal in WB without background.
Date published: 2022-02-01
Rated 5 out of 5 by from Nice and ClearGreat antibody to probe expression in various cells using pLX304 vector for expression of Cterm v5-tagged protein. No troubleshooting, worked just fine at 1:10,000 dilution primary and 1:50,000 secondary using 50ug total protein loading per well (0.2um PVDF transfer using various MW proteins)
Date published: 2019-01-29
Rated 5 out of 5 by from CompleteSuccessful completion of the target, parsing some proteins including transmembrane and secreted, were labeled and detected by immunofluorescence by Western blotting.
Date published: 2017-02-10
Rated 5 out of 5 by from Strong westernVery clear, specific WB data; strong signal with little or no background
Date published: 2017-01-26
Rated 5 out of 5 by from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB and ELISA. Strong, clear signal with little to no background. Very reliable antibody.
Date published: 2017-01-06
Rated 3 out of 5 by from Worked well in IP but not WBWorked well in IP but not WB.
Date published: 2015-10-29
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-06-01
Rated 4 out of 5 by from Publishable data in WB using human PC3 cell linesPublishable data in WB using human PC3 cell lines. -SCBT Publication Review
Date published: 2015-03-08
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V5-Probe 抗体 (C-9) is rated 4.6 out of 5 by 9.
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