Date published: 2025-9-7

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USP9 抗体 (5G-02): sc-100628

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说明书
  • USP9 抗体 5G-02 是小鼠单克隆 IgG1 κ,USP9抗体, 在5篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组USP9
  • 推荐用于 human 来源的USP9 WB, IP, IF 和 ELISA检测
  • 关于如何获取USP9 (5G-02): sc-100628免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是USP9 Antibody (5G-02) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与USP9 Antibody (5G-02) 打包提供(请参阅下面的订购信息)。

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関連項目

USP9抗体(5G-02)是一种IgG1 κ小鼠单克隆USP9抗体(也被称为USP9X抗体),可通过WB、IP、IF和ELISA检测人类来源的USP9蛋白。USP9抗体(5G-02)为非偶联抗USP9抗体。泛素(Ub)途径涉及三个连续的酶促步骤,这些步骤促进Ub和Ub样分子与特定蛋白质底物的结合。通过使用能够添加或去除泛素的多种酶,Ub途径控制着许多细胞内过程,如信号转导、转录激活和细胞周期进程。USP9(泛素特异性肽酶9),也被称为USP9X、FAF或DFFRX,是含有2547个氨基酸的肽酶C19家族泛素蛋白酶成员。USP9在胎儿和成人组织中普遍表达,参与泛素前体和泛素化蛋白质的加工处理,从而防止降解并调节蛋白质周转。编码USP9的基因缺陷与特纳综合征有关,特纳综合征是一种卵母细胞无法增殖和发育的病症,会导致发育中的卵巢退化。由于选择性剪接事件,存在编码长转录本和短转录本的多种异构体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

USP9 抗体 (5G-02) 参考文献:

  1. Usp9y(Y 上的泛素特异性蛋白酶 9 基因)与一个功能性启动子有关,并编码一个与 Usp9x 同源的完整开放阅读框,该阅读框受到选择性限制。  |  Hall, NM., et al. 2003. Mamm Genome. 14: 437-47. PMID: 12925892
  2. 双皮质素与泛素蛋白酶 DFFRX 相互作用,后者与神经元过程中的微管缔合。  |  Friocourt, G., et al. 2005. Mol Cell Neurosci. 28: 153-64. PMID: 15607950
  3. 参与突触发育的泛素特异性蛋白酶 Usp9x 在小鼠海马中的空间和时间特异性表达  |  Xu, J., et al. 2005. J Neurosci Res. 80: 47-55. PMID: 15723417
  4. 小鼠大脑中 Usp9x 蛋白表达和 DNA 甲基化与年龄有关的变化  |  Xu, J. 2005. Brain Res Mol Brain Res. 140: 17-24. PMID: 16023255
  5. 14-3-3 与 LKB1 合作调节 QSK 和 SIK 的活性和定位。  |  Al-Hakim, AK., et al. 2005. J Cell Sci. 118: 5661-73. PMID: 16306228
  6. 泛素连接酶 itch 在体内和体外都会被自动泛素化,但通过与去泛素化酶 FAM/USP9X 相互作用而免于降解。  |  Mouchantaf, R., et al. 2006. J Biol Chem. 281: 38738-47. PMID: 17038327
  7. USP9X 和非典型 Lys(29)/Lys(33)-linked 多泛素链对 AMPK 相关激酶的控制。  |  Al-Hakim, AK., et al. 2008. Biochem J. 411: 249-60. PMID: 18254724
  8. 人类 DFFRY 基因的编码序列特征和精细图谱,以及 Dffry 基因的比较表达分析和与小鼠 Y 染色体 Sxrb 区间的图谱。  |  Brown, GM., et al. 1998. Hum Mol Genet. 7: 97-107. PMID: 9384609

订购信息

产品名称产品编号规格价格数量收藏夹

USP9 抗体 (5G-02)

sc-100628
100 µg/ml
$333.00

USP9 (5G-02): m-IgG Fc BP-HRP 套装

sc-537280
100 µg Ab; 10 µg BP
$354.00

USP9 (5G-02): m-IgGκ BP-HRP 套装

sc-534511
100 µg Ab; 40 µg BP
$354.00

USP9 (5G-02): m-IgG1 BP-HRP 套装

sc-545123
100 µg Ab; 20 µg BP
$354.00

A double band around 290K is detected using USP9 antibody in Western blot. (Double bands are also seen in the sample image as well.) Which one is the band of USP9? Also, if one is a non-specific band, can I buy a blocking peptide for USP9?

Asked by: Nori
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I am using USP9 (5G-02): sc-100628 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great for Western BlotAntibody has been used to detect USP9 in epidermoid carcinoma cell line AW8507 and squamous cell carcinoma cell line AW13516 and SCC029B by western blot in published research. -SCBT QC
Date published: 2023-09-14
Rated 3 out of 5 by from Good specificity in WB in HeLa nuclear extractGood specificity in WB in HeLa nuclear extract. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by from Published data for WB in HEK293T cellsPublished data for WB in HEK293T cells. -SCBT Publication Review
Date published: 2015-04-25
Rated 3 out of 5 by from Decent immunofluorescence cytoplasmic stainingDecent immunofluorescence cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-01-13
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USP9 抗体 (5G-02) is rated 4.0 out of 5 by 4.
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