Date published: 2025-9-6

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USP47 抗体 (4E7): sc-100633

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说明书
  • USP47 抗体 4E7 是小鼠单克隆 IgG2a κ,USP47抗体, 在10篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组USP47
  • 推荐用于 mouse, rat 和 human 来源的USP47 WB, IP, IF 和 ELISA检测
  • 关于如何获取USP47 (4E7): sc-100633免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成USP47 抗体 (4E7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

USP47抗体(4E7)是一种IgG2a κ小鼠单克隆USP47抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的USP47蛋白。USP47抗体(4E7)为非偶联抗USP47抗体形式。泛素(Ub)途径涉及三个连续的酶促步骤,这些步骤促进Ub和Ub样分子与特定蛋白质底物的结合。通过使用能够添加或去除泛素的多种酶,Ub途径控制着许多细胞内过程,如信号转导、转录激活和细胞周期进程。USP47(泛素特异性肽酶47),也被称为TRFP(Trf(TATA结合蛋白相关因子)-近端同源物),是一种含有1375个氨基酸的蛋白质,属于肽酶C19蛋白家族。USP47在骨骼肌、睾丸和心脏中表达,包含作为去泛素化酶所需的所有活性残基,但似乎没有催化活性。由于选择性剪接事件,USP47有三种异构体表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

USP47 抗体 (4E7) 参考文献:

  1. 人类和小鼠蛋白酶:基因组比较方法。  |  Puente, XS., et al. 2003. Nat Rev Genet. 4: 544-58. PMID: 12838346
  2. 22 种新型人类泛素特异性蛋白酶的克隆和酶学分析。  |  Quesada, V., et al. 2004. Biochem Biophys Res Commun. 314: 54-62. PMID: 14715245
  3. 对 APP 处理过程中泛素连接酶的全基因组分析发现了 BACE1 mRNA 水平的新调节因子。  |  Espeseth, AS., et al. 2006. Mol Cell Neurosci. 33: 227-35. PMID: 16978875
  4. 通过阵列比较基因组杂化体评估小儿髓母细胞瘤的全基因组拷贝数异常。  |  Lo, KC., et al. 2007. Brain Pathol. 17: 282-96. PMID: 17465989
  5. Dkk3-Cre BAC 转基因小鼠品系:视网膜祖细胞中高效基因缺失的工具。  |  Sato, S., et al. 2007. Genesis. 45: 502-7. PMID: 17661397
  6. 靶向 USP47 可克服酪氨酸激酶抑制剂的抗药性,并清除慢性骨髓性白血病中的白血病干细胞/祖细胞。  |  Lei, H., et al. 2021. Nat Commun. 12: 51. PMID: 33397955
  7. USP47去泛素化Groucho/TLE,以促进Wnt-β-catenin信号传导。  |  Kassel, S., et al. 2023. Sci Signal. 16: eabn8372. PMID: 36749823
  8. 去泛素化酶USP47可减弱病毒诱导的I型干扰素信号。  |  Chen, HY., et al. 2023. Int Immunopharmacol. 118: 110040. PMID: 37001379
  9. USP47的结构和功能特征揭示了抑制剂设计的一个热点。  |  Shin, SC., et al. 2023. Commun Biol. 6: 970. PMID: 37740002
  10. USP47抑制m6A依赖性c-Myc翻译,以维持调节性T细胞的代谢和功能平衡。  |  Wang, A., et al. 2023. J Clin Invest. 133: PMID: 37788092
  11. USP47稳定YBX1,促进急性髓细胞性白血病的发展。  |  Lei, H., et al. 2024. Oncogene. 43: 539-542. PMID: 38104157
  12. 小鼠USP47缺陷可调节肿瘤浸润免疫细胞,增强前列腺癌的抗肿瘤免疫反应。  |  Wang, QL., et al. 2024. Cancer Immunol Immunother. 73: 143. PMID: 38832955

订购信息

产品名称产品编号规格价格数量收藏夹

USP47 抗体 (4E7)

sc-100633
100 µg/ml
$333.00

Is this antibody raised using the full USP47 protein or just a fragment of it? And if just a fragment, could I know from which residues or at least if N-term or C-term? Thanks a lot!

Asked by: GloLC
Thank you for your question. This mouse monoclonal, USP47 (4E7): sc-100633 is raised against partial recombinant USP47 protein, corresponding to amino acids 203-302 of human origin {accession number, Q96K76}.
Answered by: Tech Service
Date published: 2017-03-31

Can this antibody be used to detect USP47 in equine samples?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 9
Date published: 2017-01-18
  • y_2025, m_8, d_27, h_4CST
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Rated 5 out of 5 by from Great AntibodyI tried this antibody on human, mouse, and rat cell lines and it worked beautifully in all- no non-specific bands.
Date published: 2017-12-22
Rated 5 out of 5 by from Strong productVery nice western blot data; strong signal; also clean IF staining data
Date published: 2017-02-01
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of USP47 expression in HeLa whole cell lysate.
Date published: 2017-01-10
Rated 4 out of 5 by from Good specificity in WB in HeLa whole cell lysateGood specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-23
Rated 4 out of 5 by from Good immunofluorescence cytoplasmic stainingGood immunofluorescence cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-20
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USP47 抗体 (4E7) is rated 4.6 out of 5 by 5.
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