Date published: 2025-9-6

021-6093-6350

SCBT Portrait Logo
Seach Input

USP4 抗体 (H-3): sc-376000

4.8(6)
寫評論提問

说明书
  • USP4 抗体 H-3 是小鼠单克隆 IgG1 κ,USP4抗体, 在10篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列708-779位于human物种的USP4的内部区域内
  • USP4 抗体 (H-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 USP4
  • 抗USP4抗体(H-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取USP4 (H-3): sc-376000免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是USP4 Antibody (H-3) for WB and IHC(P) applications. 的首选辅助检测试剂,这些试剂现与USP4 Antibody (H-3) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

USP4抗体(H-3)是一种IgG1 κ小鼠单克隆USP4抗体(也称为UNP抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的USP4蛋白。USP4抗体(H-3)既有非偶联型抗USP4抗体形式,也有多种偶联形式的抗USP4抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。泛素(Ub)途径涉及三个连续的酶促步骤,促进Ub和Ub样分子与特定蛋白质底物的结合。泛素途径通过使用一系列能够添加或去除泛素的酶来控制许多细胞内过程,如信号转导、转录激活和细胞周期进程。USP4(泛素特异性加工蛋白酶4),也称为泛素羧基末端水解酶4、UNP或UNPH(泛在核蛋白同源物),是一种属于肽酶C19家族的963个氨基酸的核胞质蛋白。USP4与Gs偶联受体腺苷A2A-R的C末端结合,增强功能活性受体的细胞表面表达。USP4含有一个DUSP结构域,并由于选择性剪接而存在两种异构体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

USP4 抗体 (H-3) 参考文献:

  1. 去泛素化酶 Unp 与视网膜母细胞瘤蛋白相互作用。  |  DeSalle, LM., et al. 2001. Oncogene. 20: 5538-42. PMID: 11571652
  2. 致癌小鼠 UNP/USP4 去泛素化酶的核-细胞质穿梭。  |  Soboleva, TA., et al. 2005. J Biol Chem. 280: 745-52. PMID: 15494318
  3. 泛素特异性蛋白酶 Usp4 可调节 A2A 受体的细胞表面水平。  |  Milojevic, T., et al. 2006. Mol Pharmacol. 69: 1083-94. PMID: 16339847
  4. 腺苷受体找到了新伙伴,并搬了出去。  |  Toews, ML. 2006. Mol Pharmacol. 69: 1075-8. PMID: 16421289
  5. UnpEL/Usp4 被 Ro52 泛素化,而自身则去泛素化。  |  Wada, K. and Kamitani, T. 2006. Biochem Biophys Res Commun. 342: 253-8. PMID: 16472766
  6. 两个信号的尾巴:A(2A)-腺苷受体的 C 末端招募替代信号途径。  |  Gsandtner, I. and Freissmuth, M. 2006. Mol Pharmacol. 70: 447-9. PMID: 16707626
  7. A(2A)- 腺苷受体:具有独特特征的 GPCR?  |  Zezula, J. and Freissmuth, M. 2008. Br J Pharmacol. 153 Suppl 1: S184-90. PMID: 18246094
  8. 位于 3p21.31 的人类 UNP 基因座编码两种具有组织选择性的, 具有去泛素活性的细胞质异构体,它们在小细胞肺癌细胞系中的表达量减少。  |  Frederick, A., et al. 1998. Oncogene. 16: 153-65. PMID: 9464533
  9. 去泛素化酶:一类新的生物调节剂。  |  D'Andrea, A. and Pellman, D. 1998. Crit Rev Biochem Mol Biol. 33: 337-52. PMID: 9827704

订购信息

产品名称产品编号规格价格数量收藏夹

USP4 抗体 (H-3)

sc-376000
200 µg/ml
$316.00

USP4 (H-3): m-IgG Fc BP-HRP 套装

sc-527499
200 µg Ab; 10 µg BP
$354.00

USP4 (H-3): m-IgG1 BP-HRP 套装

sc-532872
200 µg Ab; 20 µg BP
$354.00

USP4 抗体 (H-3) AC

sc-376000 AC
500 µg/ml, 25% agarose
$416.00

USP4 抗体 (H-3) HRP

sc-376000 HRP
200 µg/ml
$316.00

USP4 抗体 (H-3) FITC

sc-376000 FITC
200 µg/ml
$330.00

USP4 抗体 (H-3) PE

sc-376000 PE
200 µg/ml
$343.00

USP4 抗体 (H-3) Alexa Fluor® 488

sc-376000 AF488
200 µg/ml
$357.00

USP4 抗体 (H-3) Alexa Fluor® 546

sc-376000 AF546
200 µg/ml
$357.00

USP4 抗体 (H-3) Alexa Fluor® 594

sc-376000 AF594
200 µg/ml
$357.00

USP4 抗体 (H-3) Alexa Fluor® 647

sc-376000 AF647
200 µg/ml
$357.00

USP4 抗体 (H-3) Alexa Fluor® 680

sc-376000 AF680
200 µg/ml
$357.00

USP4 抗体 (H-3) Alexa Fluor® 790

sc-376000 AF790
200 µg/ml
$357.00

I am using USP4 (H-3): sc-376000 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_376000, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 101ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from it is a negative result in squamous cell carcinomaI got this about half year ago. It was reported that usp4 had strong expression in squamous cell carcinoma and melonama. However, our experience found that this indenx did not express in squamous cell carcinoma. At first, I thought this product was out of date, and then, it proved to be ok because we got positve result in melonama.
Date published: 2018-04-04
Rated 5 out of 5 by from Great antibody!1:200 diluted for wb of rat protein ,the band is quite strong and special!
Date published: 2017-12-30
Rated 5 out of 5 by from Beautiful IF staining with HeLa cellsBeautiful IF staining with HeLa cells. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear andExceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human skeletal muscle tissue. -SCBT QC
Date published: 2015-04-30
Rated 5 out of 5 by from Robust staining in paraffinRobust staining in paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-04-30
Rated 5 out of 5 by from WB: Very strong band detected in KWB: Very strong band detected in K-562 samples. -SCBT QC
Date published: 2015-02-25
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_376000, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
USP4 抗体 (H-3) is rated 4.8 out of 5 by 6.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_376000, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT