Date published: 2025-10-11

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uricase 抗体 (C-11): sc-166214

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说明书
  • uricase抗体(C-11)是小鼠单克隆IgG2a λ uricase抗体, 在3篇文献中引用,规格为200 µg/ml
  • 免疫mouse物种的uricase的氨基酸1-303
  • uricase 抗体 (C-11) 推荐用于 WB, IP, IF 和 ELISA,检测mouse 和rat 来源的 uricase
  • 抗uricase抗体(C-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体,可用于WB、IHC(P)和ELISA实验
  • 2a BP-HRP">m-IgG2a BP-HRPm-IgGλ BP-HRP是uricase Antibody (C-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与uricase Antibody (C-11) 打包提供(请参阅下面的订购信息)。

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関連項目

尿酸酶抗体 (C-11) 是一种小鼠单克隆 IgG2a λ 轻链抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和酶联免疫吸附 (ELISA) 检测小鼠和大鼠尿酸酶蛋白。尿酸酶抗体 (C-11) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。尿酸酶又称尿酸氧化酶,通过催化尿酸氧化成尿囊素(一种更容易排出体外的可溶性化合物),在嘌呤代谢中发挥着至关重要的作用。这种酶的功能尤为重要,因为尿酸水平升高会导致痛风和肾结石等疾病。在大多数哺乳动物体内,尿酸酶主要存在于肝脏中,定位于过氧物酶体内,并以四聚体形式存在,这对酶的活性至关重要。有趣的是,人类和某些灵长类动物由于基因突变而缺乏这种酶,而这种基因突变在进化过程中发生得相对较晚,这对这些物种的尿酸水平管理产生了影响。编码尿酸酶的基因位于人类染色体 1p22,了解该基因的结构和功能有助于深入了解与嘌呤代谢有关的代谢紊乱,如与嘌呤挽救途径缺陷有关的莱希-尼汉综合征。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

uricase 抗体 (C-11) 参考文献:

  1. 人类尿酸氧化酶基因(UOX)在 1p22 的定位。  |  Yeldandi, AV., et al. 1992. Cytogenet Cell Genet. 61: 121-2. PMID: 1395718
  2. 拉斯布酶治疗急性高尿酸血症和肾功能障碍。  |  Ronco, C., et al. 2005. Contrib Nephrol. 147: 115-123. PMID: 15604611
  3. 尿酸盐氧化酶:主要结构和进化意义。  |  Wu, XW., et al. 1989. Proc Natl Acad Sci U S A. 86: 9412-6. PMID: 2594778
  4. 通过在小鼠体内引入 HPRT 突变,建立雷希─奈恩综合征的潜在动物模型。  |  Kuehn, MR., et al. Nature. 326: 295-8. PMID: 3029599
  5. 通过培养细胞的种系定植获得的 HPRT 缺失(莱希-尼汉)小鼠胚胎。  |  Hooper, M., et al. Nature. 326: 292-5. PMID: 3821905

订购信息

产品名称产品编号规格价格数量收藏夹

uricase 抗体 (C-11)

sc-166214
200 µg/ml
$316.00

uricase (C-11): m-IgGλ BP-HRP 套装

sc-521535
200 µg Ab, 40 µg BP
$354.00

uricase (C-11): m-IgG2a BP-HRP 套装

sc-546234
200 µg Ab; 10 µg BP
$354.00

uricase 抗体 (C-11) AC

sc-166214 AC
500 µg/ml, 25% agarose
$416.00

uricase 抗体 (C-11) HRP

sc-166214 HRP
200 µg/ml
$316.00

uricase 抗体 (C-11) FITC

sc-166214 FITC
200 µg/ml
$330.00

uricase 抗体 (C-11) PE

sc-166214 PE
200 µg/ml
$343.00

uricase 抗体 (C-11) Alexa Fluor® 488

sc-166214 AF488
200 µg/ml
$357.00

uricase 抗体 (C-11) Alexa Fluor® 546

sc-166214 AF546
200 µg/ml
$357.00

uricase 抗体 (C-11) Alexa Fluor® 594

sc-166214 AF594
200 µg/ml
$357.00

uricase 抗体 (C-11) Alexa Fluor® 647

sc-166214 AF647
200 µg/ml
$357.00

uricase 抗体 (C-11) Alexa Fluor® 680

sc-166214 AF680
200 µg/ml
$357.00

uricase 抗体 (C-11) Alexa Fluor® 790

sc-166214 AF790
200 µg/ml
$357.00

uricase 抗体 (C-11) B

sc-166214 B
200 µg/ml
$320.00

What is the specific immunogen used to generate this antibody? Is it Candida, bacillus, or something else? Will it be reactive against candida Uricase?

Asked by: Soumen
Thank you for your question. For assistance please contact technical service . Please email scbt@scbt.com or call 800-457-3801.
Answered by: BlakeJ
Date published: 2025-02-07

What is the recommended fixation method for immunofluorescence staining with uricase (C-11): sc-166214 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great AbI have used this antibody for WB and have confirmed with my transgenic line. Works perfect!
Date published: 2017-09-26
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmicProduced superb immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-01-11
Rated 5 out of 5 by from Good Western Blot data of uricase expressionGood Western Blot data of uricase expression in non-transfected and mouse uricase transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-03-24
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uricase 抗体 (C-11) is rated 5.0 out of 5 by 3.
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