Tom20抗体 (F-10) 推荐用于 mouse, rat 和 human 来源的Tom20 WB, IP, IF, IHC(P) 和 ELISA检测

Tom20 抗体 (F-10): sc-17764

Tom20抗体 (F-10) is rated 4.4 out of 5 by 60.
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说明书

    • 抗-Tom20抗体(F-10)是小鼠单克隆IgG2a λ Tom20抗体, 在358篇文献中引用,规格为200 µg/ml
    • raised against amino acids 1-145 of Tom20 human origin
    • 抗-Tom20 抗体 (F-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Tom20
    • 抗-Tom20 抗体 (F-10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Tom20 (F-10): sc-17764免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary reagent for Tom20 Antibody (F-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with Tom20 Antibody (F-10) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.

this antibody should replace the old polyclonal "sc-11415" antibody. However, this one is mouse, and i am looking for an antibody coming from rabbit. Is it possible to still order the old antibody?

Asked by: Marisaa
Thank you for your question. Unfortunately we do not offer sc-11415, and are unable to provide it for you. We are a monoclonal specific company and do not provide rabbit antibodies at this time.
Answered by: Tech Service
Date published: 2019-10-14

What is the minimum amount of protein required for signal? I am currently loading 5 ug of protein as this is ideal for the other Abs I am working with. I have tried to dilute the TOM20 F-10 antibody at 1:500 and 1:100 without a hint of a band.

Asked by: Twan
Thank you for your question. We recommend a starting dilution of 1:100 for TOM20 antibodies. We suggest loading at least 40-60µg of protein on the gel. You can find the recommended protocol here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Tech Service
Date published: 2019-08-27

Dear SCBT, I am a new group leader at the MPI for Biology of Ageing in Cologne and am interested in mitochondrial antibodies. Could you please send me a sample of the Tom20 (F-10)? Many thanks! Lena

Asked by: lpernas
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-02-05

Can we use this for IP. has anybody tried this for IP experiments

Asked by: Reshu
Thank you for your question. Yes, this antibody has been successfully published for use in immunoprecipitation (IP) applications. For example, please refer to the following product citations, PMID's: 27562021, 21732913 and 24999239.
Answered by: Technical Support
Date published: 2018-12-15

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2016-12-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-15

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: jerojero
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody Tom20 Antibody (F-10): sc-17764 is currently available conjugated to HRP (sc-17764 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; and to either phycoerythrin (sc-17764 PE) or fluoroscein (sc-17764 FITC), Alexa Fluor® 488 (sc-17764 AF488) or Alexa Fluor® 647 (sc-17764 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 3
Date published: 2016-12-15
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Rated 4 out of 5 by from Precipitation problems! Very good for IF We´ve been doing IF with this antibody with excelent results, is really usefull. But then we haven´t used it for 10 months (during the pandemia) and now we found it´s completely dried and precipitated. We diluted the precipitate and used it with good results but in a few weeks at 4°C it precipitated again. Do you have any advice? We really need it and we steel have half of the vial. Thanks
Date published: 2021-01-19
Rated 5 out of 5 by from Nice mitochondrias after Immunofluorescence Primary Fibroblasts were used to check the mitochondrial network for a downstream FISH application. 1:150 dilution was used.
Date published: 2020-12-07
Rated 4 out of 5 by from buon funzionamento The antibody anti TOM20 was tested by immunofluorescence on cells derived from human mesothelioma. It was used at a 1:250 dilution, followed by incubation with AlexaFluor 555 secondary antibody; both at RT in the dark. The analysis through fluorescence microscopy showed a good staining
Date published: 2020-10-14
Rated 5 out of 5 by from Tom20 are useful in immunofluorescence and WB Tom20 is a good mouse monoclonal antibody. We use it as one of the markers of mitochondrial protein and can get good results in WB test. Mitochondrial subcellular localization can also be performed well in cellular immunofluorescence experiments, and results are better in human-derived cells than in mouse-derived cells.
Date published: 2020-10-07
Rated 5 out of 5 by from Excellent in western blotting Western blotting HepG2 cell lysate dilution 1:1000 stron and specific signal at the expected molecular weight
Date published: 2020-06-05
Rated 1 out of 5 by from Non funziona bene Ho testato questo anticorpo sia per WB che per IF e in entrambi i casi non ha funzionato bene. Nel blot ho rilevato un segnale sporco e in IF avevo un segnale diffuso. In entrambi i casi hon usato cellule Neuro2a.
Date published: 2020-02-07
Rated 1 out of 5 by from Didn't work The staining did not working after methanol or PFA fixation at 1:50 for mouse dendritic cells.
Date published: 2019-12-28
Rated 4 out of 5 by from reliable immunocitochemistry We used this antibody to stain mitochondria in melanoma cells. It worked well at 1:50 dilution after FA fixation.
Date published: 2019-09-13
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