Date published: 2025-9-6
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TNFα-IP 2 Antibody (F-6) - Western Blotting - Image 356881
TNFα-IP 2 抗体 (F-6): sc-28318. HeLa (A) 和 A549 (B) 全细胞裂解液及人扁桃体组织提取物 (C) 中 TNFα-IP 2 表达的 Western 印迹分析.
TNFα-IP 2 抗体 (F-6): sc-28318. HeLa (A) 和 A549 (B) 全细胞裂解液及人扁桃体组织提取物 (C) 中 TNFα-IP 2 表达的 Western 印迹分析.

TNFα-IP 2 抗体 (F-6): sc-28318

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说明书
  • TNF alpha-IP 2 抗体 F-6 是小鼠单克隆 IgG1 κ，TNFα-IP 2抗体, 在21篇文献中引用，规格为200 µg/ml
  • 与氨基酸1-654结合，代表全长human起源的TNFα诱导蛋白2
  • TNF alpha-IP 2 抗体 (F-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TNFα-IP 2
  • 抗TNF alpha-IP 2抗体（F-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TNFα-IP 2 (F-6): sc-28318的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 TNFα-IP 2 抗体 (F-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TNFα-IP 2 抗体 (F-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 TNFα-IP 抗体 包括 TNF α-IP 2

TNFα-IP 2抗体（F-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的TNFα-IP 2蛋白。TNFα-IP 2 (F-6) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。TNFα-IP 2，也称为B94或TNFAIP2，是Sec6家族的一员，在炎症和血管生成中起着关键作用，这些过程对于组织修复和再生至关重要。TNFα-IP 2在发育过程中有差异性表达，在体外毛细血管样管形成中尤为重要，突出了TNFα-IP 2在血管生物学中的重要性。TNFAIP2基因位于14q32染色体上，编码一种由肿瘤坏死因子α和其他促炎因子诱导的654个氨基酸的蛋白质，使TNFα-IP 2成为炎症反应中的关键角色。TNFAIP2最初被确定为内皮细胞中诱导的基因，也被认为与多种癌症有关，包括急性早幼粒细胞白血病，其中TNFα-IP 2被维甲酸上调。抗TNFα-IP 2抗体（F-6）具有多种应用和重要的生物学功能，是研究人员研究炎症、血管生成和相关途径的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TNFα-IP 2 抗体 (F-6) 参考文献:

  1. 鉴定 B94（TNFAIP2）是急性早幼粒细胞白血病中潜在的视黄酸靶基因。  |  Rusiniak, ME., et al. 2000. Cancer Res. 60: 1824-9. PMID: 10766166
  2. 利用人类基因组序列确定了在一名 39 岁女性的致命宫颈癌中整合到 TNFAIP2 基因的 16 型人类乳头瘤病毒 DNA 的位置。  |  Einstein, MH., et al. 2002. Clin Cancer Res. 8: 549-54. PMID: 11839676
  3. 利用寡核苷酸阵列比较分析受 PML/RAR alpha 和 PLZF/RAR alpha 调控的基因对维甲酸的反应。  |  Park, DJ., et al. 2003. Blood. 102: 3727-36. PMID: 12893766
  4. 微阵列分析发现人类支气管上皮细胞中的维甲酸靶标  |  Ma, Y., et al. 2003. Oncogene. 22: 4924-32. PMID: 12894236
  5. 克隆一种新型肿瘤坏死因子-α诱导的主要反应基因，该基因在体外发育和毛细管样形成过程中有不同表达。  |  Sarma, V., et al. 1992. J Immunol. 148: 3302-12. PMID: 1374453
  6. 大鼠 6q32 染色体上 4 Mb 区间的 1 型糖尿病--保护基因的同源图谱。  |  Klöting, N. and Klöting, I. 2004. Biochem Biophys Res Commun. 323: 388-94. PMID: 15369764
  7. TNFAIP2 在癌症和传染病中的作用。  |  Jia, L., et al. 2018. J Cell Mol Med. 22: 5188-5195. PMID: 30145807
  8. 隧道纳米管-TNFAIP2/M-sec系统对糖尿病肾病荚膜细胞自噬的保护作用  |  Barutta, F., et al. 2023. Autophagy. 19: 505-524. PMID: 35659195
  9. TNFAIP2 通过 KEAP1/NRF2 信号传递赋予头颈部鳞状细胞癌顺铂抗性。  |  Xu, T., et al. 2023. J Exp Clin Cancer Res. 42: 190. PMID: 37525222
  10. hnRNP E1和E2在调节HIV-1基因表达方面具有不同的作用。  |  Fang, H., et al. 2023. Elife. 12: PMID: 37787041
  11. TNFAIP2 作为癌症治疗中的新兴治疗靶点及其内在机制。  |  Fu, Z. and Yuan, Y. 2024. Pharmacol Res. 204: 107199. PMID: 38688431
  12. B94 是肿瘤坏死因子-α 诱导的主要反应基因，在发育中的造血组织和精子顶体中表达。  |  Wolf, FW., et al. 1994. J Biol Chem. 269: 3633-40. PMID: 8106408

订购信息

产品名称产品编号规格价格数量收藏夹

TNFα-IP 2 抗体 (F-6)

sc-28318
200 µg/ml
$316.00

TNFα-IP 2 (F-6): m-IgG Fc BP-HRP 套装

sc-528420
200 µg Ab; 10 µg BP
$354.00

TNFα-IP 2 (F-6): m-IgGκ BP-HRP 套装

sc-520794
200 µg Ab, 40 µg BP
$354.00

TNFα-IP 2 (F-6): m-IgG1 BP-HRP 套装

sc-542922
200 µg Ab; 20 µg BP
$354.00

TNFα-IP 2 抗体 (F-6) AC

sc-28318 AC
500 µg/ml, 25% agarose
$416.00

TNFα-IP 2 抗体 (F-6) HRP

sc-28318 HRP
200 µg/ml
$316.00

TNFα-IP 2 抗体 (F-6) FITC

sc-28318 FITC
200 µg/ml
$330.00

TNFα-IP 2 抗体 (F-6) PE

sc-28318 PE
200 µg/ml
$343.00

TNFα-IP 2 抗体 (F-6) Alexa Fluor® 488

sc-28318 AF488
200 µg/ml
$357.00

TNFα-IP 2 抗体 (F-6) Alexa Fluor® 546

sc-28318 AF546
200 µg/ml
$357.00

TNFα-IP 2 抗体 (F-6) Alexa Fluor® 594

sc-28318 AF594
200 µg/ml
$357.00

TNFα-IP 2 抗体 (F-6) Alexa Fluor® 647

sc-28318 AF647
200 µg/ml
$357.00

TNFα-IP 2 抗体 (F-6) Alexa Fluor® 680

sc-28318 AF680
200 µg/ml
$357.00

TNFα-IP 2 抗体 (F-6) Alexa Fluor® 790

sc-28318 AF790
200 µg/ml
$357.00

Good afternoon, I would like to know the epitope recognised by the following antibody. I am doing knock out experiments and see by sequencing that my gene is knock-out but I still detect the protein by western blot which is a bit confusing. Thanks

Asked by: Thomas
Thank you for your question. TNFα-IP 2 Antibody (F-6): sc-28318 was raised against amino acids 1-654 representing full length TNFα-induced protein 2 of human origin. No epitope mapping has been performed for this antibody as of yet, so its exact binding site is currently not known.
Answered by: Tech Support Europe
Date published: 2024-11-08

Dear Mr, Mrs, I am interesting in an TnfaIP2 antibody for WB with mouse organ tissue. I already tried 2 antibody that didnt gave me good results (unspecific band). Can I have a try with yours, by a 10ug free sample ? This Ab in necessary for my PhD thesis

Asked by: Louis Nabineau
Thank you for your interest in our antibody sample program. To request a sample please contact technical service by emailing scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-08-06

Can TNFα-IP 2 (F-6): sc-28318 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TNFα-IP 2 (F-6): sc-28318 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works very wellThis antibody was great for Western blotting in mouse heart tissue
Date published: 2024-01-07
Rated 1 out of 5 by from can't work on WBI have tried antibody dilution 1:1000, 1:500, 1:300, 1:100 on A549 whole cell lysate with western blot staining, but the band is unclear and lots of unspecific bands.
Date published: 2018-04-16
Rated 5 out of 5 by from F-6 clone works very well in IF stainingI got this free sample and try it on my mouse skin staining, it works very well. I also tested it one flow cytometry. The outcome is also good, even though it is less ideal compared to IF staining.
Date published: 2017-08-14
Rated 4 out of 5 by from good to useDiluted 1:300 at first, It has a good band at 73 kDa.
Date published: 2017-07-17
Rated 5 out of 5 by from WB resultThe target band is very strong and intensely by antibody dilution 1:800,I suggest use Hela cell as a positive control first while test the sample which are not used by other researchers.
Date published: 2017-07-02
Rated 5 out of 5 by from clear and specificI use this atnibody for WB,Whihc showed that the bands were clear,no-background, and ideal. I am responsible to say that TNFα-IP 2 Antibody is quality controled and very reliable.
Date published: 2017-07-01
Rated 5 out of 5 by from Robust signalRobust signal in WB in human transfected 293T and Jurkat whole cell lysates.
Date published: 2017-05-13
Rated 5 out of 5 by from Great to useDiluted 1:500, It has good band at 73 kDa.very good
Date published: 2017-05-07
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TNFα-IP 2 抗体 (F-6) is rated 4.6 out of 5 by 12.
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