Date published: 2025-9-5

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TMEFF2 抗体 (E-12): sc-376175

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说明书
  • TMEFF2 抗体 E-12 是小鼠单克隆 IgG2a (kappa轻链),TMEFF2抗体,规格为200 µg/ml
  • 免疫原氨基酸序列41-103位于human物种的TMEFF2的N-terminus附近
  • TMEFF2 抗体 (E-12) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 TMEFF2; 也和以下物种反应,包括: equine
  • 抗TMEFF2抗体(E-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TMEFF2 (E-12): sc-376175免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成TMEFF2 抗体 (E-12)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    TMEFF2 抗体(E-12)是一种 IgG2a κ 小鼠单克隆 TMEFF2 抗体(也称为 TMEFF2 抗体),可通过 WB、IP、IF 和 ELISA 检测小鼠、大鼠和人类来源的 TMEFF2 蛋白。TMEFF2 抗体(E-12)既可以是非偶联的抗 TMEFF2 抗体形式,也可以是多种偶联形式的抗 TMEFF2 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。含有表皮生长因子和两个卵泡抑素结构域的跨膜蛋白(TMEFF1 和 TMEFF2)是单次跨膜的1型膜蛋白,由一个表皮生长因子(EGF)样结构域和两个卵泡抑素样结构域组成。TMEFF2 也被称为增生性息肉病蛋白 1(HPP1)或托莫瑞格林(TR),可能作为中脑和海马神经元的生存因子。它在脑、前列腺和脊髓中高表达,但也可以在结肠和正常结肠粘膜的基质细胞中检测到。TMEFF2 是一种高度糖基化的蛋白,由于其 5' 区域的甲基化,在肿瘤细胞系中表达下调。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    TMEFF2 抗体 (E-12) 参考文献:

    1. 一种含有两个follistatin模块的新型表皮生长因子样分子可刺激MKN28胃癌细胞中erbB-4的酪氨酸磷酸化。  |  Uchida, T., et al. 1999. Biochem Biophys Res Commun. 266: 593-602. PMID: 10600548
    2. 海马和间脑神经元的新型存活因子 TMEFF2 的鉴定和特征描述  |  Horie, M., et al. 2000. Genomics. 67: 146-52. PMID: 10903839
    3. 一种含有表皮生长因子和follistatin结构域的新型跨膜蛋白的基因在人类肿瘤细胞中经常发生高甲基化。  |  Liang, G., et al. 2000. Cancer Res. 60: 4907-12. PMID: 10987305
    4. HPP1:大肠息肉和癌症中常见的甲基化跨膜蛋白编码基因。  |  Young, J., et al. 2001. Proc Natl Acad Sci U S A. 98: 265-70. PMID: 11120884
    5. TENB2,一种在前列腺癌中发现的与疾病进展和雄激素独立性缔合作用的蛋白多糖。  |  Glynne-Jones, E., et al. 2001. Int J Cancer. 94: 178-84. PMID: 11668495
    6. 抗 TMEFF2-auristatin E 结合物抗体治疗前列腺癌的临床前验证。  |  Afar, DE., et al. 2004. Mol Cancer Ther. 3: 921-32. PMID: 15299075

    订购信息

    产品名称产品编号规格价格数量收藏夹

    TMEFF2 抗体 (E-12)

    sc-376175
    200 µg/ml
    $316.00

    TMEFF2 抗体 (E-12) AC

    sc-376175 AC
    500 µg/ml, 25% agarose
    $416.00

    TMEFF2 抗体 (E-12) HRP

    sc-376175 HRP
    200 µg/ml
    $316.00

    TMEFF2 抗体 (E-12) FITC

    sc-376175 FITC
    200 µg/ml
    $330.00

    TMEFF2 抗体 (E-12) PE

    sc-376175 PE
    200 µg/ml
    $343.00

    TMEFF2 抗体 (E-12) Alexa Fluor® 488

    sc-376175 AF488
    200 µg/ml
    $357.00

    TMEFF2 抗体 (E-12) Alexa Fluor® 546

    sc-376175 AF546
    200 µg/ml
    $357.00

    TMEFF2 抗体 (E-12) Alexa Fluor® 594

    sc-376175 AF594
    200 µg/ml
    $357.00

    TMEFF2 抗体 (E-12) Alexa Fluor® 647

    sc-376175 AF647
    200 µg/ml
    $357.00

    TMEFF2 抗体 (E-12) Alexa Fluor® 680

    sc-376175 AF680
    200 µg/ml
    $357.00

    TMEFF2 抗体 (E-12) Alexa Fluor® 790

    sc-376175 AF790
    200 µg/ml
    $357.00

    Is the epitope this antibody binds to intracellular? Is permeabilization of the cell membrane necessary? Thank you!

    Asked by: Stru1111
    Thank you for your question. It would be helpful if you could call us directly at 800- 457-3801 option 2, allowing for a more interactive discussion of this and other related questions.
    Answered by: Blake J
    Date published: 2021-08-04

    I have bought Anti-TMEFF2 Antibody (E-12) PE label, Can use this Ab for FLowcytometry.If so I need to know the concentration.

    Asked by: karthickbalan
    Thank you for your question. Anti-TMEFF2 Antibody (E-12) PE can be used for FCM. We recommend using 1 µg of fluorochrome-conjugated antibodies for 100 µl of the prepared cell suspension (equal to 1 million cells).
    Answered by: Tech Support Europe
    Date published: 2021-07-26

    Are there any issues with using sc-376175: TMEFF2 (E-12) mouse monoclonal primary antibody on mouse samples?

    Asked by: Trav11
    Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-376175 conjugates.
    Answered by: Technical Support
    Date published: 2017-03-07
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    Rated 5 out of 5 by from Great Antibody for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Beautiful IF staining. Works well in a variety of species including mouse, rat, and human samples.
    Date published: 2017-01-20
    Rated 5 out of 5 by from Produced nice Western blot data of TMEFF2Produced nice Western blot data of TMEFF2 expression in LNCaP whole cell lysate. -SCBT QC
    Date published: 2014-04-16
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    TMEFF2 抗体 (E-12) is rated 5.0 out of 5 by 2.
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