Date published: 2025-9-7
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TLE4 Antibody (E-10) - Western Blotting - Image 372693
TLE4 抗体 (E-10): sc-365406. 小鼠大脑 (A) 和大鼠大脑 (B) 组织提取物中 TLE4 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
TLE4 抗体 (E-10): sc-365406. 小鼠大脑 (A) 和大鼠大脑 (B) 组织提取物中 TLE4 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

TLE4 抗体 (E-10): sc-365406

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说明书
  • TLE4 抗体 E-10 是小鼠单克隆 IgG1 κ，TLE4抗体, 在31篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的TLE4的氨基酸273-473
  • TLE4 抗体 (E-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TLE4
  • 抗TLE4抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-365406 X, 200 µg/0.1 ml)
  • 关于如何获取TLE4 (E-10): sc-365406的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 TLE4 抗体 (E-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TLE4 抗体 (E-10) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 TLE 抗体 包括 TLE1, TLE2, TLE3, TLE4, TLE5和TLE6

TLE4抗体（E-10）是一种IgG1 κ型小鼠单克隆TLE4抗体（也称为Groucho相关蛋白4（GRG4）抗体），它能够通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC(P)）和酶联免疫吸附测定（ELISA）检测到来自小鼠、大鼠和人类的TLE4蛋白。TLE4抗体（E-10）既提供非偶联的抗TLE4抗体形式，也提供多种偶联形式的抗TLE4抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。Notch信号通路控制细胞间的相互作用，对于无脊椎动物和脊椎动物中多种命运的特化具有重要意义。Notch通路中的关键角色是TLE基因（代表分裂的类传导素增强子，也称为ESG，代表增强子分裂的groucho），它们是人类果蝇groucho基因的同源物。Groucho是一种转录抑制因子，在神经发生、分节和性别决定中起关键作用。TLEs与活细胞中的染色质以及特异性的组蛋白H3相关联，但不与其他核心组蛋白相关联。TLE基因（TLE1、TLE2、TLE3和TLE4）的表达与正在向终末分化状态发展的未成熟上皮细胞相关，这表明它们在上皮分化过程中具有作用。TLE1、TLE2和TLE3在宫颈鳞状上皮化生和癌中的表达升高，而TLE4在大脑中的表达最高，特别是在尾状核中。TLE1和TLE4包含SP和WD40结构域，通过这些结构域，TLE1与AML1结合，抑制AML1诱导的CSF1受体反式激活。在细胞分化的早期阶段，TLE1上调，而TLE2和TLE4下调。在后期阶段，TLE2和TLE4上调，而TLE1的表达减少。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TLE4 抗体 (E-10) 参考文献:

  1. 果蝇分裂增强基因产物的人类同源物定义了一个新的核蛋白家族。  |  Stifani, S., et al. 1992. Nat Genet. 2: 119-27. PMID: 1303260
  2. 通过高密度寡核苷酸阵列, 逆转录酶定量 PCR 和蛋白质表达分析垂体癌和腺瘤的基因表达模式。  |  Ruebel, KH., et al. 2006. Endocrine. 29: 435-44. PMID: 16943582
  3. Groucho/Tle在左右不对称和Kupffer小泡器官发生中的作用  |  Bajoghli, B., et al. 2007. Dev Biol. 303: 347-61. PMID: 17188260
  4. Nkx2.2-抑制因子的活性足以指定胰岛中的α细胞和少量β细胞。  |  Doyle, MJ., et al. 2007. Development. 134: 515-23. PMID: 17202186
  5. TLE4是成骨细胞和Runx2依赖性骨骼发育的关键调节因子。  |  Shin, TH., et al. 2021. Front Cell Dev Biol. 9: 671029. PMID: 34422801
  6. TLE4调节肌肉干细胞静止和骨骼肌分化。  |  Agarwal, M., et al. 2022. J Cell Sci. 135: PMID: 35099008
  7. Tle4控制皮质丘脑投射神经元的发育获得和出生后早期成熟。  |  Galazo, MJ., et al. 2023. Cell Rep. 42: 112957. PMID: 37561632
  8. 果蝇的神经发生, 分节和性别决定都需要 Groucho，它与毛发相关的 bHLH 蛋白直接相互作用。  |  Paroush, Z., et al. 1994. Cell. 79: 805-15. PMID: 8001118
  9. 人类 TLE 基因的上皮表达和染色体位置：对缺口信号转导和肿瘤的影响。  |  Liu, Y., et al. 1996. Genomics. 31: 58-64. PMID: 8808280
  10. Groucho/transducin-like 增强子分裂转录抑制因子与组蛋白 H3 的基因定义氨基末端沉默结构域相互作用。  |  Palaparti, A., et al. 1997. J Biol Chem. 272: 26604-10. PMID: 9334241
  11. 细胞决定和分化过程中单个 TLE 蛋白的组合表达模式表明，果蝇 Groucho 的哺乳动物同源物具有非冗余功能。  |  Yao, J., et al. 1998. Dev Growth Differ. 40: 133-46. PMID: 9572356
  12. AML1 和 LEF-1 的转录抑制是由 TLE/Groucho 核心抑制因子介导的。  |  Levanon, D., et al. 1998. Proc Natl Acad Sci U S A. 95: 11590-5. PMID: 9751710

订购信息

产品名称产品编号规格价格数量收藏夹

TLE4 抗体 (E-10)

sc-365406
200 µg/ml
$316.00

TLE4 (E-10): m-IgG Fc BP-HRP 套装

sc-529371
200 µg Ab; 10 µg BP
$354.00

TLE4 (E-10): m-IgGκ BP-HRP 套装

sc-522237
200 µg Ab, 40 µg BP
$354.00

TLE4 (E-10): m-IgG1 BP-HRP 套装

sc-543483
200 µg Ab; 20 µg BP
$354.00

TLE4 抗体 (E-10) X

sc-365406 X
200 µg/0.1 ml
$316.00

TLE4 抗体 (E-10) AC

sc-365406 AC
500 µg/ml, 25% agarose
$416.00

TLE4 抗体 (E-10) HRP

sc-365406 HRP
200 µg/ml
$316.00

TLE4 抗体 (E-10) FITC

sc-365406 FITC
200 µg/ml
$330.00

TLE4 抗体 (E-10) PE

sc-365406 PE
200 µg/ml
$343.00

TLE4 抗体 (E-10) Alexa Fluor® 488

sc-365406 AF488
200 µg/ml
$357.00

TLE4 抗体 (E-10) Alexa Fluor® 546

sc-365406 AF546
200 µg/ml
$357.00

TLE4 抗体 (E-10) Alexa Fluor® 594

sc-365406 AF594
200 µg/ml
$357.00

TLE4 抗体 (E-10) Alexa Fluor® 647

sc-365406 AF647
200 µg/ml
$357.00

TLE4 抗体 (E-10) Alexa Fluor® 680

sc-365406 AF680
200 µg/ml
$357.00

TLE4 抗体 (E-10) Alexa Fluor® 790

sc-365406 AF790
200 µg/ml
$357.00

Are there articles using (sc-365406) TLE4 Antibody (E-10) for rat brain in the citation list ?

Asked by: KAZU
Thank you for your question. We are very sorry, we do not have that data at the moment. If you have any questions you can contact your dealer. Thank you.
Answered by: Jiajun Z
Date published: 2024-10-09

Does TLE4 Antibody (E-10): sc-365406 react with non-human primate samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 15
Date published: 2017-01-24
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Rated 1 out of 5 by from Not working on human post-mortex prefrontal cortexI bought this antibody a few weeks ago, I tested it on human post-mortem FFPE prefrontal cortex tissue, with very scarse results. The sections were dewaxed with 2x5’ xylene, rehydrated with 2x5’ 100%, 5’ 90% and 5’ 70% EtOH and washed with dH2O 2x10’. Antigen retrieval was performed with EDTA pH9 20’ at 95C. Sections were then let cool down 10’ in ice, then washed 10’ in dH2O and 10’ in PBS. Blocking was performed with blocking buffer (10% donkey serum in PBS with 0.3%Triton and 0.003% Sodium Azide) for 1hr at RT. Anti-TLE4 antibody was diluted 1:200 in blocking buffer and incubated at 4C for 1 night. Anti-mouse Alexa 647 secondary antibody was then applied after 3x 5’ washed in PBS, diluted 1:500 in blocking buffer with DAPI, for 1 hr at RT. Sections were then washed 3x 5’ in PBS, stained with Sudan black for 10’, washed again in dH2O, air dried and mounted. Confocal acquistion of the staining is attached: TLE4 seems to stain most of nuclei, with no layer specificity. I can't use this antibody for my studies and I would like to get a refund.
Date published: 2022-05-30
Rated 5 out of 5 by from TLE antibodyI purchased this antibody for the IF on the kidney tissues. It showed pretty good and highly recommend it on the IF.
Date published: 2019-10-27
Rated 5 out of 5 by from Works in mouseThis antibody works very well in both IF and WB for mouse TLE4.
Date published: 2019-09-04
Rated 5 out of 5 by from GreatThis antibody work very well for both IF and western blot
Date published: 2017-09-06
Rated 5 out of 5 by from Great antibody!Works great for IF on brain tissue! Dilution is 1:200.
Date published: 2017-02-22
Rated 5 out of 5 by from Published for ChIP/WBTLE4 Antibody (E-10) was featured in a 2016 publication for ChIP and immunoblotting, PMID: 27301576.
Date published: 2016-12-28
Rated 5 out of 5 by from Produced superb immunoperoxidase nuclearProduced superb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Nice Western Blot data of TLE4 expressionNice Western Blot data of TLE4 expression in P19 whole cell lysate. -SCBT QC
Date published: 2014-12-04
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TLE4 抗体 (E-10) is rated 4.5 out of 5 by 8.
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