Date published: 2025-9-7

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Tyrosine Hydrolase 抗体 (F-11): sc-25269

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说明书
  • Tyrosine Hydrolase 抗体 F-11 是小鼠单克隆 IgG2a κ,Tyrosine Hydrolase抗体, 在204篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1-196(在31-61处有缺失)的序列,在human起源的TH处进行映射
  • Tyrosine Hydrolase 抗体 (F-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 TH
  • 抗Tyrosine Hydrolase抗体(F-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Tyrosine Hydrolase (F-11): sc-25269免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 Tyrosine Hydrolase 抗体 (F-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Tyrosine Hydrolase 抗体 (F-11) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

TH抗体(F-11)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的TH蛋白。TH抗体(F-11)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。酪氨酸羟化酶(TH)又称酪氨酸单加氧酶,对于儿茶酚胺生物合成至关重要,催化酪氨酸转化为左旋多巴,这是生产多巴胺、肾上腺素和去甲肾上腺素等神经递质的限速步骤。这一功能在神经健康中起着至关重要的作用,因为儿茶酚胺合成失调与包括帕金森病在内的多种疾病有关,其中多巴胺水平降低会导致运动和认知障碍。TH基因调控涉及转录因子结合位点,例如TPA反应元件和c-AMP反应元件,它们对于基因表达响应生理信号至关重要。胶质细胞成熟因子和其他调节蛋白可上调TH,这凸显了TH在正常生理和疾病状态中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Tyrosine Hydrolase 抗体 (F-11) 参考文献:

  1. 酸性成纤维细胞生长因子和儿茶酚胺协同上调发育中和受损多巴胺培养神经元的酪氨酸羟化酶活性。  |  Stull, ND. and Iacovitti, L. 1996. J Neurochem. 67: 1519-24. PMID: 8858935
  2. 儿茶酚胺的合成与释放。概述。  |  Nagatsu, T. and Stjärne, L. 1998. Adv Pharmacol. 42: 1-14. PMID: 9327833
  3. 酪氨酸羟化酶与帕金森病  |  Haavik, J. and Toska, K. 1998. Mol Neurobiol. 16: 285-309. PMID: 9626667
  4. 大鼠酪氨酸羟化酶启动子的 CRE 和 TRE 序列是成年小鼠 TH 基础表达所必需的,但在胚胎中并非如此。  |  Trocmé, C., et al. 1998. Eur J Neurosci. 10: 508-21. PMID: 9749713
  5. 在 PC12 嗜铬细胞瘤细胞中过表达胶质成熟因子(GMF)可激活 p38 MAP 激酶, MAPKAP 激酶-2 和酪氨酸羟化酶。  |  Zaheer, A. and Lim, R. 1998. Biochem Biophys Res Commun. 250: 278-82. PMID: 9753620
  6. 一种含有 Cdc10/SWI6 motifs 的新型蛋白质可调控编码儿茶酚胺生物合成酶的 mRNA 的表达。  |  Yamakuni, T., et al. 1998. J Biol Chem. 273: 27051-4. PMID: 9765218
  7. TH9.0-LacZ转基因小鼠慢性吗啡对酪氨酸羟化酶启动子活性的调控。  |  Boundy, VA., et al. 1998. J Neurosci. 18: 9989-95. PMID: 9822754

订购信息

产品名称产品编号规格价格数量收藏夹

Tyrosine Hydrolase 抗体 (F-11)

sc-25269
200 µg/ml
$316.00

Tyrosine Hydrolase (F-11): m-IgG Fc BP-HRP 套装

sc-528391
200 µg Ab; 10 µg BP
$354.00

Tyrosine Hydrolase (F-11): m-IgGκ BP-HRP 套装

sc-520757
200 µg Ab, 40 µg BP
$354.00

Tyrosine Hydrolase (F-11): m-IgG2a BP-HRP 套装

sc-547082
200 µg Ab; 10 µg BP
$354.00

Tyrosine Hydrolase 抗体 (F-11) AC

sc-25269 AC
500 µg/ml, 25% agarose
$416.00

Tyrosine Hydrolase 抗体 (F-11) HRP

sc-25269 HRP
200 µg/ml
$316.00

Tyrosine Hydrolase 抗体 (F-11) FITC

sc-25269 FITC
200 µg/ml
$330.00

Tyrosine Hydrolase 抗体 (F-11) PE

sc-25269 PE
200 µg/ml
$343.00

Tyrosine Hydrolase 抗体 (F-11) Alexa Fluor® 488

sc-25269 AF488
200 µg/ml
$357.00

Tyrosine Hydrolase 抗体 (F-11) Alexa Fluor® 546

sc-25269 AF546
200 µg/ml
$357.00

Tyrosine Hydrolase 抗体 (F-11) Alexa Fluor® 594

sc-25269 AF594
200 µg/ml
$357.00

Tyrosine Hydrolase 抗体 (F-11) Alexa Fluor® 647

sc-25269 AF647
200 µg/ml
$357.00

Tyrosine Hydrolase 抗体 (F-11) Alexa Fluor® 680

sc-25269 AF680
200 µg/ml
$357.00

Tyrosine Hydrolase 抗体 (F-11) Alexa Fluor® 790

sc-25269 AF790
200 µg/ml
$357.00

what is the recommended secondary antibody for IF for this primary antibody?

Asked by: Anonymous
Thank you for your question. You can use any fluorochrome-conjugated secondary that is specific against this antibody's kappa light chain, such as m-IgGκ BP-CFL 488: sc-516176 or m-IgGκ BP-CFL 647: sc-516179. You can find additional secondary binding proteins under this link: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Support Europe
Date published: 2020-08-19

Which animal the Anti-TH Antibody (F-11) raised?  Thanks 

Asked by: Anonymous
Thank you for your question. This antibody was raised in mouse. Anti-TH Antibody (F-11) is a mouse monoclonal IgG2a.
Answered by: Technical Support
Date published: 2020-03-11

Can TH Antibody (F-11): sc-25269 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, TH Antibody (F-11): sc-25269 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 5 out of 5 by from Great results in IHC in mouse brainperfect co-staining of dopaminergic, TH positive neurons (red) and the synaptic active zone protein bassoon (green) in mouse striatum !
Date published: 2022-06-02
Rated 5 out of 5 by from good for immunostaining and Western blottingno background, very sensitive, using brain samples
Date published: 2021-07-30
Rated 5 out of 5 by from Very good antibody for Western blot applicationThis antibody is excellent for TH detection by Western blot. It was employed to evaluate TH expression in differentiating N1E-115 cell line, and we obtained a strong and specific signal with no background. Antibody dilution: 1:1000 in PBST with 5% nonfat dry milk.
Date published: 2021-02-17
Rated 4 out of 5 by from Buon funzionamentoBuon funzionamento dell'anticorpo alla condizione 1:50 ph6
Date published: 2020-11-19
Rated 5 out of 5 by from Great product!Quick shipment. Reliable product! But too much email followup to ask for feedback-a little bit embarrassed.
Date published: 2020-09-12
Rated 5 out of 5 by from Strong and specific staining in IFThe reagents gives strong and specific signal on mouse heart cryosections at the dilution range of 1:200-1:400.
Date published: 2020-04-15
Rated 4 out of 5 by from Antibody reviewThis antibody worked for immunofluorescence staining with fixed frozen mouse tissue.
Date published: 2018-11-16
Rated 4 out of 5 by from Antibody reviewThis antibody can be used for Western blotting at 1:500 dilution with appropriate mouse tissue lysate.
Date published: 2018-06-14
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Tyrosine Hydrolase 抗体 (F-11) is rated 4.7 out of 5 by 37.
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