TGase1 Antibody (B-3): sc-377076
- TGase1 Antibody (B-3) is a mouse monoclonal IgM κ provided at 200 µg/ml
- raised against amino acids 731-817 mapping at the C-terminus of TGase1 of human origin
- recommended for detection of TGase1 of mouse, rat and human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TGase1 Antibody (B-3). This work is in progress.
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TGase1 Antibody (B-3) is a mouse monoclonal IgM antibody that detects TGase1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TGase1, also known as human keratinocyte transglutaminase, is a crucial enzyme involved in the formation of the cross-linked cell envelope, a vital component of the epidermis that provides structural integrity and barrier function to the skin. This 817 amino acid protein is membrane-associated and plays a significant role in epidermal differentiation by catalyzing the formation of isopeptide bonds through the action of transglutaminases, which are calcium-dependent enzymes that facilitate the cross-linking of glutamine and lysine residues in substrate proteins. The proper functioning of TGase1 is essential for maintaining skin homeostasis and protecting against environmental stressors, as TGase1 contributes to the formation of a robust barrier that prevents water loss and pathogen entry. Dysregulation of TGase1 activity has been implicated in various skin disorders, highlighting TGase1′s importance in dermatological health. Anti-TGase1 antibody (B-3) is an invaluable tool for researchers studying the mechanisms of skin biology and the role of transglutaminases in cellular processes.
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TGase1 Antibody (B-3) References:
- Facilitated wound healing by activation of the Transglutaminase 1 gene. | Inada, R., et al. 2000. Am J Pathol. 157: 1875-82. PMID: 11106560
- Characterization of human recombinant transglutaminase 1 purified from baculovirus-infected insect cells. | Hitomi, K., et al. 2000. Biosci Biotechnol Biochem. 64: 2128-37. PMID: 11129585
- Chronic ultraviolet radiation modulates epidermal differentiation as it up-regulates transglutaminase 1 and its substrates. | Lee, DS., et al. 2005. Photodermatol Photoimmunol Photomed. 21: 45-52. PMID: 15634223
- Mutant huntingtin protein: a substrate for transglutaminase 1, 2, and 3. | Zainelli, GM., et al. 2005. J Neuropathol Exp Neurol. 64: 58-65. PMID: 15715085
- Novel transglutaminase-1 mutations and genotype-phenotype investigations of 104 patients with autosomal recessive congenital ichthyosis in the USA. | Farasat, S., et al. 2009. J Med Genet. 46: 103-11. PMID: 18948357
- A specific colorimetric assay for measuring transglutaminase 1 and factor XIII activities. | Hitomi, K., et al. 2009. Anal Biochem. 394: 281-3. PMID: 19646949
- Novel transglutaminase 1 mutations in patients affected by lamellar ichthyosis. | Terrinoni, A., et al. 2012. Cell Death Dis. 3: e416. PMID: 23096117
- Topical enzyme-replacement therapy restores transglutaminase 1 activity and corrects architecture of transglutaminase-1-deficient skin grafts. | Aufenvenne, K., et al. 2013. Am J Hum Genet. 93: 620-30. PMID: 24055110
- Tissue transglutaminase-1 promotes stemness and chemoresistance in gastric cancer cells by regulating Wnt/β-catenin signaling. | Huang, H., et al. 2017. Exp Biol Med (Maywood). 242: 194-202. PMID: 27660242
- A gene-centric approach to biomarker discovery identifies transglutaminase 1 as an epidermal autoantigen. | Landegren, N., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34911754
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGase1 Antibody (B-3) | sc-377076 | 200 µg/ml | $322.00 |