Date published: 2025-9-10

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TBK1 抗体 (108A429): sc-52957

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说明书
  • TBK1抗体(108A429)是小鼠单克隆IgG1 κ, 在25篇文献中引用,规格为50 µg/0.5 ml
  • 与human的TBK1的氨基酸563-577对应的合成肽杂交
  • 推荐用于 mouse, rat, human, bovine 和 canine 来源的TBK1 WB, IP 和 ELISA检测
  • 关于如何获取TBK1 (108A429): sc-52957免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是TBK1 Antibody (108A429) for WB applications. 的首选辅助检测试剂,这些试剂现与TBK1 Antibody (108A429) 打包提供(请参阅下面的订购信息)。

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関連項目

TBK1抗体(108A429)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠、人类、牛和犬源性TBK1。抗TBK1抗体(108A429)以非结合形式提供。TBK1,也称为TANK结合激酶1,在调节NFκB信号通路中起着至关重要的作用,对免疫反应和炎症至关重要。TBK1主要位于细胞质中,与各种信号分子相互作用,调节NFκB活性。TBK1的细胞质定位使其能够快速响应细胞应激和感染,促进IκB蛋白磷酸化,从而导致IκB降解和随后的NFκB释放和激活。这一过程对于参与免疫反应的基因转录至关重要,使TBK1成为防御机制中的关键角色。TBK1还与TRAF2和TANK相互作用,进一步突出了TBK1在NFκB激活途径中的作用。包括TBK1、IKKα和IKKβ在内的功能性IKK复合物强调了这些激酶在调节关键细胞过程中的协同作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TBK1 抗体 (108A429) 参考文献:

  1. 包含 TRAF2, TANK 和 TBK1(一种新型 IKK 相关激酶)的信号复合体激活 NF-kappaB。  |  Pomerantz, JL. and Baltimore, D. 1999. EMBO J. 18: 6694-704. PMID: 10581243
  2. TBK1 在发育和衰老过程中抑制 RIPK1 驱动的细胞凋亡和炎症。  |  Xu, D., et al. 2018. Cell. 174: 1477-1491.e19. PMID: 30146158
  3. TBK1和IKKε在介导STING诱导的髓样细胞NF-κB反应中发挥冗余作用  |  Balka, KR., et al. 2020. Cell Rep. 31: 107492. PMID: 32268090
  4. TBK1-METTL3轴促进抗病毒免疫。  |  Chen, J., et al. 2022. Cell Rep. 38: 110373. PMID: 35172162
  5. 以 TBK1 为靶点,克服癌症免疫疗法的抗药性。  |  Sun, Y., et al. 2023. Nature. 615: 158-167. PMID: 36634707
  6. Rel/NF-kappa B/I kappa B 家族:关联与分离的亲密故事。  |  Verma, IM., et al. 1995. Genes Dev. 9: 2723-35. PMID: 7590248
  7. NF-kappa B:家庭价值观的一课。  |  Thanos, D. and Maniatis, T. 1995. Cell. 80: 529-32. PMID: 7867060
  8. CHUK是螺旋-环-螺旋和亮氨酸拉链相互作用蛋白家族的新成员,含有丝氨酸-苏氨酸激酶催化结构域。  |  Connelly, MA. and Marcu, KB. 1995. Cell Mol Biol Res. 41: 537-49. PMID: 8777433
  9. IkappaB 激酶的鉴定和表征。  |  Régnier, CH., et al. 1997. Cell. 90: 373-83. PMID: 9244310
  10. 一种细胞因子反应型 IkappaB 激酶,可激活转录因子 NF-kappaB。  |  DiDonato, JA., et al. 1997. Nature. 388: 548-54. PMID: 9252186
  11. IkappaB 激酶复合物(IKK)包含两个激酶亚基 IKKalpha 和 IKKbeta,它们是 IkappaB 磷酸化和 NF-kappaB 激活所必需的。  |  Zandi, E., et al. 1997. Cell. 91: 243-52. PMID: 9346241
  12. NEMO 的补全克隆,NEMO 是 IkappaB 激酶复合物的一个组成部分,对 NF-kappaB 激活至关重要。  |  Yamaoka, S., et al. 1998. Cell. 93: 1231-40. PMID: 9657155

订购信息

产品名称产品编号规格价格数量收藏夹

TBK1 抗体 (108A429)

sc-52957
50 µg/0.5 ml
$316.00

TBK1 (108A429): m-IgG Fc BP-HRP 套装

sc-538964
50 µg Ab; 10 µg BP
$354.00

TBK1 (108A429): m-IgG1 BP-HRP 套装

sc-541181
50 µg Ab; 20 µg BP
$354.00

What is the best secondary to use with this antibody in Western Blot?

Asked by: Anna99
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-17
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Rated 2 out of 5 by from western blotting: a lot of aspecific bandsWestern blotting dilution 1:1000 CFBE cells Strong aspecific signal at about 50 kDa. A band at the expcted molecular weigh appears only covering part of the blot (weak signal)
Date published: 2020-07-01
Rated 5 out of 5 by from Reliable!I bought the antibody 2 months ago and we’re very please with the results. Happy with it only takes 2 hours room temperature incubation and the bands are clear. Good product!
Date published: 2019-12-21
Rated 5 out of 5 by from Great antibody!I received this antibody a few months ago and it works perfectly. Give a clear and strong band.
Date published: 2018-09-13
Rated 5 out of 5 by from very good for WBIt worked well for WB of human protein in cell lysate. 1:1000 dilution in 3% BSA, PBST. The antibody detected TBK1 band very strongly.
Date published: 2018-02-23
Rated 2 out of 5 by from Non specific bandsI requested for a free sample of TBK 1 antibody. But the results were not satisfactory as I got some non specific bands.
Date published: 2018-01-04
Rated 4 out of 5 by from Works for WBI have tested this on mouse liver lysates. it works ok but seems to have non specific bands too.
Date published: 2017-07-12
Rated 5 out of 5 by from Excellent TBK1 AntibodyTBK1 (108A429) good banding for Western blot (WB) at 80 kDa. Recommend MCF7 Whole Cell Lysate: sc-2206 as a positive control.
Date published: 2017-01-11
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using MCF7, THP-1, NTERA-2 cl.D1 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-07-06
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TBK1 抗体 (108A429) is rated 4.4 out of 5 by 11.
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