Date published: 2025-9-8
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Syk Antibody (4D10) - Western Blotting - Image 372650
Syk 抗体 (4D10): sc-1240. BJAB (A), A-431 (B), THP-1 (C), Ramos (D) 和 NAMALWA (E) 全细胞裂解液中 Syk 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
Syk 抗体 (4D10): sc-1240. BJAB (A), A-431 (B), THP-1 (C), Ramos (D) 和 NAMALWA (E) 全细胞裂解液中 Syk 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Syk 抗体 (4D10): sc-1240

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说明书
  • Syk 抗体 4D10 是小鼠单克隆 IgG2a κ，Syk抗体, 在269篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Syk的氨基酸313-339
  • Syk 抗体 (4D10) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Syk
  • 抗Syk抗体（4D10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Syk (4D10): sc-1240的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Syk Antibody (4D10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Syk Antibody (4D10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Syk抗体（4D10）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测小鼠、大鼠和人类来源的Syk蛋白。Syk (4D10) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Syk蛋白由635个氨基酸组成，具有一个蛋白激酶结构域和两个SH2结构域，是蛋白激酶超家族的重要成员。Syk在B细胞信号传导中起着至关重要的作用，它作为B细胞抗原受体（CD79）刺激反应的积极效应器，通过两种不同的磷酸化调节途径促进钙离子运动。当未磷酸化时，钙离子通过磷脂酰肌醇3激酶依赖性途径，而酪氨酸残基348和352的磷酸化将钙离子流转移到磷脂酶Cγ依赖性途径。这种调节机制对于各种细胞过程至关重要，包括分化、吞噬作用、增殖和B细胞发育。T细胞淋巴瘤中Syk的表达上调，表明其可能参与肿瘤发生。Syk有两种异构体，即短型和长型，由可变剪接事件产生，这可能会导致细胞信号传导通路的功能多样性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Syk 抗体 (4D10) 参考文献:

  1. 72-kDa蛋白酪氨酸激酶PTK72与B细胞抗原受体的关联。  |  Hutchcroft, JE., et al. 1992. J Biol Chem. 267: 8613-9. PMID: 1569106
  2. Syk 是细胞间黏合剂分子-1 的下游，介导人鼻病毒激活气道上皮细胞中的 p38 MAPK。  |  Wang, X., et al. 2006. J Immunol. 177: 6859-70. PMID: 17082600
  3. 失调的 Syk 会抑制前 B 细胞的分化，并诱导生长因子依赖性增殖。  |  Wossning, T., et al. 2006. J Exp Med. 203: 2829-40. PMID: 17130299
  4. 酪氨酸激酶 Syk 与收费样受体 4 缔合作用并调节人类单核细胞的信号传导。  |  Chaudhary, A., et al. 2007. Immunol Cell Biol. 85: 249-56. PMID: 17228323
  5. CD303 (BDCA-2) 通过涉及 Syk, Slp65 和 PLCgamma2 的 BCR 类信号组在浆细胞树突状细胞中发出信号。  |  Röck, J., et al. 2007. Eur J Immunol. 37: 3564-75. PMID: 18022864
  6. 蛋白激酶 C-delta 激活 Syk 可通过 RelA/p65 的酪氨酸磷酸化调节凝血酶诱导的内皮细胞细胞间粘附分子-1 的表达。  |  Bijli, KM., et al. 2008. J Biol Chem. 283: 14674-84. PMID: 18362147
  7. 外周 T 细胞淋巴瘤中 Syk 酪氨酸激酶的过度表达。  |  Feldman, AL., et al. 2008. Leukemia. 22: 1139-43. PMID: 18401419
  8. 丙型肝炎病毒 NS5A 蛋白与非受体蛋白酪氨酸激酶 Syk 相互作用并对其进行负向调节。  |  Inubushi, S., et al. 2008. J Gen Virol. 89: 1231-1242. PMID: 18420802
  9. 吉非替尼和福沙坦尼可靶向EGFR和SYK，从而减轻矽肺：一项探索药物的多组学研究。  |  Wang, M., et al. 2022. Signal Transduct Target Ther. 7: 157. PMID: 35551173
  10. SYK在神经退行性疾病中协调神经保护性小胶质细胞反应。  |  Ennerfelt, H., et al. 2022. Cell. 185: 4135-4152.e22. PMID: 36257314
  11. TREM2通过SYK依赖性和非依赖性途径驱动小胶质细胞对淀粉样蛋白-β的反应  |  Wang, S., et al. 2022. Cell. 185: 4153-4169.e19. PMID: 36306735
  12. Syk 蛋白-酪氨酸激酶受酪氨酸磷酸化的 Ig alpha/Ig beta 免疫受体酪氨酸激活基团结合和自身磷酸化的调控。  |  Rowley, RB., et al. 1995. J Biol Chem. 270: 11590-4. PMID: 7538118

订购信息

产品名称产品编号规格价格数量收藏夹

Syk 抗体 (4D10)

sc-1240
200 µg/ml
$316.00

Syk (4D10): m-IgG Fc BP-HRP 套装

sc-528128
200 µg Ab; 10 µg BP
$354.00

Syk (4D10): m-IgGκ BP-HRP 套装

sc-520431
200 µg Ab, 40 µg BP
$354.00

Syk (4D10): m-IgG2a BP-HRP 套装

sc-547029
200 µg Ab; 10 µg BP
$354.00

Syk 抗体 (4D10) AC

sc-1240 AC
500 µg/ml, 25% agarose
$416.00

Syk 抗体 (4D10) HRP

sc-1240 HRP
200 µg/ml
$316.00

Syk 抗体 (4D10) FITC

sc-1240 FITC
200 µg/ml
$330.00

Syk 抗体 (4D10) PE

sc-1240 PE
200 µg/ml
$343.00

Syk 抗体 (4D10) Alexa Fluor® 488

sc-1240 AF488
200 µg/ml
$357.00

Syk 抗体 (4D10) Alexa Fluor® 546

sc-1240 AF546
200 µg/ml
$357.00

Syk 抗体 (4D10) Alexa Fluor® 594

sc-1240 AF594
200 µg/ml
$357.00

Syk 抗体 (4D10) Alexa Fluor® 647

sc-1240 AF647
200 µg/ml
$357.00

Syk 抗体 (4D10) Alexa Fluor® 680

sc-1240 AF680
200 µg/ml
$357.00

Syk 抗体 (4D10) Alexa Fluor® 790

sc-1240 AF790
200 µg/ml
$357.00

How to grade the positivity in breast carcinoma cells

Asked by: Ghazala
Thank you for your question. You would require appropriate positive and negative controls to compare the signals between different samples. Recommended positive controls are NAMALWA cell lysate: sc-2234, BJAB whole cell lysate: sc-2207 or Raji whole cell lysate: sc-364236. We also recommend using direct conjugates for detection so that signal strength directly corresponds to the amount of protein present.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Is it possible for the Syk Antibody (4D10): sc-1240 to react with monkey tissue samples?

Asked by: Trav11
To inquire about additional species reactivity not listed on the webpage, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function. We will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 5 out of 5 by from Syk Antibody (4D10)Syk Antibody (4D10) (sc-1024) worked well in our Western blotting experiments.
Date published: 2021-01-22
Rated 4 out of 5 by from Detects SYK in cell lines with low baseline levelsI tested SYK in a cell line with low baseline levels (according to CCLE). This antibody was able to detect bands at a dilution of 1:1000.
Date published: 2020-10-08
Rated 4 out of 5 by from Works good!Good a free sample to test. Planing to use in future! For WB perferct!
Date published: 2018-02-13
Rated 5 out of 5 by from Great antibody for western blotThe batch of antibody I have worked well on HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 500 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2017-11-16
Rated 5 out of 5 by from The antibody worked in western blotThis antibody worked very well in western blot on mouse osteoclasts extract. We diluted it at 1/200 but it could be diluted more because the signal was strong.
Date published: 2017-10-25
Rated 5 out of 5 by from It works great.I tested in PC3 cells. It worked well at 1:200 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from worked well.I got this antibody as free samples and it worked very well for WB. I used 1:500 dilution.
Date published: 2017-03-15
Rated 5 out of 5 by from Excellent antibody for westernsProbed this antibody at 1/1000 dilution for one hour and used the Kappa-binding HRP as secondary: the result was flawless. Excellent antibody for westerns.
Date published: 2017-03-08
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Syk 抗体 (4D10) is rated 4.7 out of 5 by 19.
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