Date published: 2025-9-7

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SVCT1 抗体 (H-11): sc-376090

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说明书
  • SVCT1 抗体 H-11 是小鼠单克隆 IgG1 κ,SVCT1抗体, 在3篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列521-598位于human物种的SVCT1的C-terminus
  • SVCT1 抗体 (H-11) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 SVCT1
  • 抗SVCT1抗体(H-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SVCT1 (H-11): sc-376090免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 SVCT1 抗体 (H-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SVCT1 抗体 (H-11) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

SVCT1抗体(H-11)是一种IgG1 κ小鼠单克隆SVCT1抗体(也称为SVCT1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源SVCT1蛋白。SVCT1抗体(H-11)既可以是非偶联型抗SVCT1抗体形式,也可以是多种偶联型抗SVCT1抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。钠依赖性维生素C转运体SVCT1和SCVT2是L-抗坏血酸(维生素C)的膜转运体。两种SVCT蛋白均介导高亲和力Na+依赖性L-抗坏血酸转运,对于许多组织摄取维生素C是必需的。SVCT1是一种含有604个氨基酸的蛋白质,主要在上皮组织中表达,包括肠道、肾脏和肝脏。SVCT2是一种含有592个氨基酸的蛋白质,与SVCT1具有65%的同源性,已在多种代谢活性细胞以及眼和脑等特化组织中检测到。在正常人肠道中已鉴定出一种非功能性SVCT1剪接变体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SVCT1 抗体 (H-11) 参考文献:

  1. 哺乳动物 Na+依赖性 L-抗坏血酸转运体家族。  |  Tsukaguchi, H., et al. 1999. Nature. 399: 70-5. PMID: 10331392
  2. 人胎盘钠依赖性维生素 C 转运体(SVCT2):分子克隆和转运功能。  |  Rajan, DP., et al. 1999. Biochem Biophys Res Commun. 262: 762-8. PMID: 10471399
  3. 人类 Na(+)依赖性维生素 C 转运体 1(hSVCT1):一级结构, 功能特征和无功能剪接变体的证据。  |  Wang, H., et al. 1999. Biochim Biophys Acta. 1461: 1-9. PMID: 10556483
  4. 人类钠依赖性维生素 C 转运体 hSVCT1 和 hSVCT2 的克隆和功能鉴定。  |  Daruwala, R., et al. 1999. FEBS Lett. 460: 480-4. PMID: 10556521
  5. 上皮细胞中维生素C转运体SVCT1和SVCT2的极性定位。  |  Boyer, JC., et al. 2005. Biochem Biophys Res Commun. 334: 150-6. PMID: 15993839
  6. 围产期肾脏发育过程中钠-维生素C共转运体SVCT1和SVCT2的动态表达。  |  Nualart, F., et al. 2013. Histochem Cell Biol. 139: 233-47. PMID: 22990596
  7. 肝脏代谢/氧化应激诱导肝脏和肝外维生素 C 转运体 SVCT1 和 SVCT2 的表达发生变化。  |  Hierro, C., et al. 2014. Eur J Nutr. 53: 401-12. PMID: 23708151
  8. 抗坏血酸结合纳米颗粒通过钠依赖性维生素C转运体1(SVCT1)促进治疗药物的口服给药。  |  Luo, Q., et al. 2018. Artif Cells Nanomed Biotechnol. 46: 198-208. PMID: 29260899
  9. 维生素C转运蛋白SVCT1在生理上起着尿酸盐进口者的作用:功能分析和体内研究。  |  Toyoda, Y., et al. 2023. Pflugers Arch. 475: 489-504. PMID: 36749388
  10. 人类维生素C转运蛋白SVCT1的二聚体转运机制。  |  Kobayashi, TA., et al. 2024. Nat Commun. 15: 5569. PMID: 38956111
  11. 人类SVCT1的二聚体是运输功能的关键。  |  Woubshete, M., et al. 2024. Biochim Biophys Acta Biomembr. 1866: 184390. PMID: 39369805
  12. 来自人和小鼠肾脏以及 LLC-PK1 细胞的两种新型转运体的分子特征揭示了一个与细菌和曲霉核碱基转运体同源的新型保守家族。  |  Faaland, CA., et al. 1998. Biochim Biophys Acta. 1442: 353-60. PMID: 9804989

订购信息

产品名称产品编号规格价格数量收藏夹

SVCT1 抗体 (H-11)

sc-376090
200 µg/ml
$316.00

SVCT1 (H-11): m-IgG Fc BP-HRP 套装

sc-529820
200 µg Ab; 10 µg BP
$354.00

SVCT1 (H-11): m-IgGκ BP-HRP 套装

sc-522843
200 µg Ab, 40 µg BP
$354.00

SVCT1 (H-11): m-IgG1 BP-HRP 套装

sc-543706
200 µg Ab; 20 µg BP
$354.00

SVCT1 抗体 (H-11) AC

sc-376090 AC
500 µg/ml, 25% agarose
$416.00

SVCT1 抗体 (H-11) HRP

sc-376090 HRP
200 µg/ml
$316.00

SVCT1 抗体 (H-11) FITC

sc-376090 FITC
200 µg/ml
$330.00

SVCT1 抗体 (H-11) PE

sc-376090 PE
200 µg/ml
$343.00

SVCT1 抗体 (H-11) Alexa Fluor® 488

sc-376090 AF488
200 µg/ml
$357.00

SVCT1 抗体 (H-11) Alexa Fluor® 546

sc-376090 AF546
200 µg/ml
$357.00

SVCT1 抗体 (H-11) Alexa Fluor® 594

sc-376090 AF594
200 µg/ml
$357.00

SVCT1 抗体 (H-11) Alexa Fluor® 647

sc-376090 AF647
200 µg/ml
$357.00

SVCT1 抗体 (H-11) Alexa Fluor® 680

sc-376090 AF680
200 µg/ml
$357.00

SVCT1 抗体 (H-11) Alexa Fluor® 790

sc-376090 AF790
200 µg/ml
$357.00

Has this antibody been used for flowcytometry?

Asked by: hkazemi
Thank you for your question. SVCT1 Antibody (H-11): sc-376090 is recommended for detection of SVCT1 of human origin by WB, IP, IF, IHC(P) and ELISA. It has not been tested in flow cytometry. Please get in touch with our technical service to arrange for a free sample of this antibody to test it in your experimental setup.
Answered by: Technical Support Europe
Date published: 2019-04-30

I am using SVCT1 (H-11): sc-376090 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24

Hi, Does the antibody binding to the extracellular or intracellular domains or both?

Asked by: Oslem TCD
Thank you for your question. This antibody, sc-376090 SVCT1 (H-11), was raised against amino acids 521-598 mapping within a cytoplasmic domain near the C-terminal of human SVCT1 (accession#Q9UHI7). If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-01-17

Can I use this antibodies in chicken?

Asked by: dawn
Thank you for your question. This antibody has not been tested in chicken samples and I can not find any sequence for SVCT1 in chicken, so I can not do a sequence homology check for you. The exact immunogen sequence is considered proprietary. However, I can provide you with a 50 amino acid range of where the immunogen is located. SVCT1 (H-11): sc-376090 was raised against a sequence between amino acids 548-598 of SVCT1 of human origin (accession# Q9UHI7). If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2016-11-14

What is the volume or weight of one unit, you are only stating 200 ug/ml, so a concentration. Also, would you be able to supply larger amounts, say 1 milligram? Please quote.

Asked by: Eva CdM
Thank you for your inquiry. The total volume provided is 1 ml. For quotes for larger quantities, please contact our Special Orders department at specialorders@scbt.com
Answered by: Technical Service
Date published: 2016-11-03
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Rated 5 out of 5 by from Superb immunoperoxidase apical membrane andSuperb immunoperoxidase apical membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-28
Rated 5 out of 5 by from Good Western Blot data of SVCT1 expression in SHGood Western Blot data of SVCT1 expression in SH-SY5Y whole cell lysate. -SCBT QC
Date published: 2014-01-13
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SVCT1 抗体 (H-11) is rated 5.0 out of 5 by 2.
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