Date published: 2025-9-7
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SV2A Antibody (E-8) - Western Blotting - Image 367505
SV2A 抗体 (E-8): sc-376234. 大鼠小脑 (A), 小鼠小脑 (B), 大鼠大脑 (C), 人脑 (D) 和小鼠大脑 (E) 组织提取物中 SV2A 表达的 Western 印迹分析.
SV2A 抗体 (E-8): sc-376234. 大鼠小脑 (A), 小鼠小脑 (B), 大鼠大脑 (C), 人脑 (D) 和小鼠大脑 (E) 组织提取物中 SV2A 表达的 Western 印迹分析.

SV2A 抗体 (E-8): sc-376234

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寫評論提問

说明书
  • SV2A 抗体 E-8 是小鼠单克隆 IgG1 κ，SV2A抗体, 在12篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于rat物种的SV2A的N-terminus的氨基酸2-25之间
  • SV2A 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SV2A; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗SV2A抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SV2A (E-8): sc-376234的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 SV2A 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与SV2A 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 SV2 抗体 包括 SV2B

SV2A抗体（E-8）是一种IgG1 κ小鼠单克隆SV2A抗体（也称为突触囊泡2A或SV2抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的SV2A蛋白。SV2A抗体（E-8）既有非偶联形式的抗SV2A抗体，也有多种偶联形式的抗SV2A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在所有脊椎动物中，SV2蛋白是丰富的、疏水的膜糖蛋白，以两种主要亚型SV2A和SV2B，以及一种次要亚型SV2C的形式表达。SV2蛋白在大脑中的表达具有差异性，且存在于所有突触囊泡中，与递质类型无关。SV2A在皮层下区域大量表达，特别是在所有突触前神经末梢的突触囊泡中，以及大多数神经内分泌分泌颗粒中。SV2B的表达模式更为局限，仅存在于海马和皮层的一小部分突触中。SV2A和SV2B在功能上是冗余的，对于维持脊椎动物的正常脑功能至关重要。SV2A和SV2B通过调节重复刺激期间神经末梢中的细胞质Ca2+水平来介导突触传递。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SV2A 抗体 (E-8) 参考文献:

  1. SV2A 和 SV2B 在神经递质释放过程中充当多余的 Ca2+ 调节器。  |  Janz, R., et al. 1999. Neuron. 24: 1003-16. PMID: 10624962
  2. SV2C 是一种突触小泡蛋白，其定域异常受限：突触小泡蛋白家族的解剖。  |  Janz, R. and Südhof, TC. 1999. Neuroscience. 94: 1279-90. PMID: 10625067
  3. 鉴定神经细胞和内分泌细胞分泌泡特异的跨膜糖蛋白。  |  Buckley, K. and Kelly, RB. 1985. J Cell Biol. 100: 1284-94. PMID: 2579958
  4. 突触囊泡糖蛋白 2A (SV2A) 在调节癫痫发生中的治疗作用  |  Ohno, Y. and Tokudome, K. 2017. CNS Neurol Disord Drug Targets. 16: 463-471. PMID: 28393712
  5. 内分泌分泌颗粒和神经元突触囊泡有三种共同的整体膜蛋白。  |  Lowe, AW., et al. 1988. J Cell Biol. 106: 51-9. PMID: 3276713
  6. 神经退行性疾病中突触密度的成像。  |  Carson, RE., et al. 2022. J Nucl Med. 63: 60S-67S. PMID: 35649655
  7. 开发用于癫痫治疗的 SV2A 配体：左乙拉西坦、布里瓦拉西坦和帕塞伏尼的回顾。  |  Wu, PP., et al. 2024. Neurosci Bull. 40: 594-608. PMID: 37897555
  8. 阿尔茨海默氏症基因敲入小鼠的神经元转录组、tau 和突触丢失需要朊病毒蛋白。  |  Stoner, A., et al. 2023. Alzheimers Res Ther. 15: 201. PMID: 37968719
  9. 血清中的突触囊泡糖蛋白 2 A 是早期诊断阿尔茨海默病的理想生物标志物。  |  Wang, X., et al. 2024. Alzheimers Res Ther. 16: 82. PMID: 38615037
  10. 抗癫痫药物和肉毒杆菌神经毒素识别 SV2A 的结构基础。  |  Yamagata, A., et al. 2024. Nat Commun. 15: 3027. PMID: 38637505
  11. 人类神经退行性疾病中的 SV2A PET 成像。  |  Bavarsad, MS. and Grinberg, LT. 2024. Front Aging Neurosci. 16: 1380561. PMID: 38699560
  12. 大脑中含有两种形式的突触小泡蛋白 2。  |  Bajjalieh, SM., et al. 1993. Proc Natl Acad Sci U S A. 90: 2150-4. PMID: 7681585

订购信息

产品名称产品编号规格价格数量收藏夹

SV2A 抗体 (E-8)

sc-376234
200 µg/ml
$316.00

SV2A (E-8): m-IgGκ BP-HRP 套装

sc-522884
200 µg Ab, 40 µg BP
$354.00

SV2A 抗体 (E-8) AC

sc-376234 AC
500 µg/ml, 25% agarose
$416.00

SV2A 抗体 (E-8) HRP

sc-376234 HRP
200 µg/ml
$316.00

SV2A 抗体 (E-8) FITC

sc-376234 FITC
200 µg/ml
$330.00

SV2A 抗体 (E-8) PE

sc-376234 PE
200 µg/ml
$343.00

SV2A 抗体 (E-8) Alexa Fluor® 488

sc-376234 AF488
200 µg/ml
$357.00

SV2A 抗体 (E-8) Alexa Fluor® 546

sc-376234 AF546
200 µg/ml
$357.00

SV2A 抗体 (E-8) Alexa Fluor® 594

sc-376234 AF594
200 µg/ml
$357.00

SV2A 抗体 (E-8) Alexa Fluor® 647

sc-376234 AF647
200 µg/ml
$357.00

SV2A 抗体 (E-8) Alexa Fluor® 680

sc-376234 AF680
200 µg/ml
$357.00

SV2A 抗体 (E-8) Alexa Fluor® 790

sc-376234 AF790
200 µg/ml
$357.00

SV2A (E-8) 中和勝肽

sc-376234 P
100 µg/0.5 ml
$68.00

Do you know if it has bsa and azide? Does it target all species? Is it limited to just SV2 A or does it work with other variants of SV2?

Asked by: Aparajeeta
Thank you for your question. SV2A Antibody (E-8): sc-376234 is supplied as 200 µg in 1 ml PBS with 0.1 % gelatin and <0.1 % sodium azide. It is is recommended for detection of SV2A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. It is also reactive with additional species, including and equine, canine, bovine and porcine samples.
Answered by: Tech Support Europe
Date published: 2025-07-09

With antigen retrieval buffer was used to test this antibody on human tissue?

Asked by: DennisNIN
Thank you for your question. Heat at 95° C with10 mM sodium citrate buffer, pH 6.0.
Answered by: BlakeJ
Date published: 2025-02-25

Does this product come with dye when purchased like Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 ?

Asked by: Okan
Thank you for your question. Product codes sc-376234 FITC, sc-376234 PE, sc-376234 AF488, sc-376234 AF546, sc-376234 AF594, sc-376234 AF647, sc-376234 AF680, and sc-376234 AF790 are supplied as fluorescent direct conjugates. The corresponding fluorochromes are bound to the antibody, which means that no secondary antibody is required for detection.
Answered by: Tech Support Europe
Date published: 2021-12-21

What application is the blocking peptide sc-376234 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2021-10-22
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Rated 4 out of 5 by from A good SV2A antibodyA reliable SV2A antibody for WB with a right molecular weight.
Date published: 2023-04-25
Rated 4 out of 5 by from A good SV2A antibodyA reliable SV2A antibody for WB with a right molecular weight.
Date published: 2023-04-25
Rated 4 out of 5 by from A good SV2A antibodyA reliable SV2A antibody for WB with a right molecular weight.
Date published: 2023-04-25
Rated 2 out of 5 by from Funciona...pero no es bonitoFunciona... pero no es bonito. La verdad es que el resultado se parece bastante al de la foto del datasheet, aunque la señal es muy variable entre muestras, y hay que usarlo bastante concentrado.
Date published: 2022-03-10
Rated 5 out of 5 by from Worked brilliantly for IPUsed for IP in primary hippocampal cultures. Following incubation with beads coated in anti-SV2A, IP pellet, supernatant, and final wash samples were fractionated by SDS-PAGE and Western blotted onto a nitrocellulose membrane and detected using an anti-SV2A rabbit polyclonal Ab from a different supplier. No SV2A was detected in either supernatant or final wash samples, demonstrating that this Ab has a high affinity for SV2A, and is ideal for IP.
Date published: 2017-11-28
Rated 5 out of 5 by from Excellent marker for SV2AWe obtained a sample of SV2A monoclonal from SCB after difficulties with the same antibody from different providers. We used this antibody in IHC-P for calculating synaptic density in human brain tissue. The staining was incredibly consistent between samples, very easily quantifiable and led to a publication. We will continue using this marker from SCB. Very satisfied.
Date published: 2017-06-28
Rated 5 out of 5 by from Strong WB dataVery strong bands in WB at a 1:200 dilution on rat cerebellum, mouse cerebellum, rat brain, human brain and mouse brain tissue extracts
Date published: 2017-01-27
Rated 5 out of 5 by from Robust Ab marker for SV2ARobust Ab marker for SV2A. <br /> Dilution of 1:500-1:1000 for IHC-P staining of paraffin embedded human brain tissue. <br /> Would recommend this manufacturer for staining.
Date published: 2016-06-10
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SV2A 抗体 (E-8) is rated 4.3 out of 5 by 9.
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