Date published: 2025-9-9
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SSEA-4 Antibody (813-70) - Flow Cytometry - Image 5792
SSEA-4 抗体 (813-70): sc-21704. 用 SSEA-4 抗体 (813-70), 然后用 FITC 结合物山羊抗小鼠 IgG3 染色的活 NTERA-2 cl. D1 细胞的间接 FCM 分析: sc-2081.
SSEA-4 Antibody (813-70) - Flow Cytometry - Image 5792
SSEA-4 抗体 (813-70): sc-21704. 用 SSEA-4 抗体 (813-70), 然后用 FITC 结合物山羊抗小鼠 IgG3 染色的活 NTERA-2 cl. D1 细胞的间接 FCM 分析: sc-2081.
SSEA-4 Antibody (813-70) - Immunofluorescence - Image 391781
SSEA-4 抗体 (813-70) FITC: sc-21704 FITC. 直接免疫荧光染色福尔马林固定的 SW480 细胞, 显示膜定位. 用 UltraCruz® 阻断试剂阻断: sc-516214.
SSEA-4 Antibody (813-70) - Flow Cytometry - Image 9481
SSEA-4 抗体 (813-70): sc-21704. 用 SSEA-4 抗体 (813-70), 然后用 PE 结合的山羊抗小鼠 IgG3: sc-3767 染色的活 NTERA-2 cl. D1 细胞的间接 FCM 分析.
SSEA-4 抗体 (813-70): sc-21704. 用 SSEA-4 抗体 (813-70), 然后用 FITC 结合物山羊抗小鼠 IgG3 染色的活 NTERA-2 cl. D1 细胞的间接 FCM 分析: sc-2081.

SSEA-4 抗体 (813-70): sc-21704

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寫評論提問

说明书
  • SSEA-4 抗体 813-70 是小鼠单克隆 IgG3 κ，SSEA-4抗体, 在222篇文献中引用，规格为200 µg/ml
  • 针对human胚胎癌细胞系2102Ep
  • 推荐用于 mouse, rat 和 human 来源的SSEA-4 IF 和 FCM检测; 不会与 undifferentiated murine EC, ES and EG cells产生交联反应
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取SSEA-4 (813-70): sc-21704的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成SSEA-4 抗体 (813-70)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
说明书与实验方案
研究信息

関連項目

此外 SSEA 抗体 包括 SSEA-1, SSEA-3和SSEA-4

SSEA-4抗体（813-70）是一种小鼠单克隆IgG3 kappa轻链抗体，可通过免疫荧光（IF）和流式细胞术（FCM）检测小鼠、大鼠和人类来源的SSEA-4蛋白。SSEA-4 (813-70) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。SSEA-4在鉴定和表征多能干细胞方面发挥着至关重要的作用，是未分化胚胎干细胞和胚胎癌细胞的关键标记物。SSEA-4 (813-70) 单克隆抗体可识别表面定位的SSEA-4，有助于维持干细胞的未分化状态，使其无限增殖的同时保留分化潜能。抗SSEA-4抗体（813-70）通过区分未分化和已分化细胞，帮助分离和研究干细胞，支持发育生物学和再生医学研究。SSEA-4有助于细胞间相互作用和信号传导通路，对维持干细胞和分化至关重要，因此SSEA-4（813-70）单克隆抗体对于理解干细胞生物学和治疗应用具有重大价值。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SSEA-4 抗体 (813-70) 参考文献:

  1. 人类胚胎干细胞的表面抗原：培养分化过程中的变化。  |  Draper, JS., et al. 2002. J Anat. 200: 249-58. PMID: 12033729
  2. 人类胚胎癌细胞及其培养分化。  |  Andrews, PW., et al. 1987. Int J Androl. 10: 95-104. PMID: 2438234
  3. mTORC1维持了SSEA-4（+）高级别骨肉瘤的致瘤性。  |  Zhang, W., et al. 2015. Sci Rep. 5: 9604. PMID: 25853231
  4. Globo H 和 SSEA-4 在癌症发生和发展中的作用及其作为治疗靶点的潜力。  |  Sigal, DS., et al. 2022. Future Oncol. 18: 117-134. PMID: 34734786
  5. 来自乳牙的 SSEA-4 阳性牙髓干细胞及其对神经前体细胞的诱导。  |  Lopez-Lozano, AP., et al. 2022. Head Face Med. 18: 9. PMID: 35236383
  6. CAR T 细胞靶向三阴性乳腺癌的阶段特异性胚胎抗原 4 (SSEA-4) 会导致小鼠出现意想不到的靶上/靶下肿瘤毒性。  |  Pfeifer, R., et al. 2023. Int J Mol Sci. 24: PMID: 37298141
  7. 糖磷脂 SSEA-4 靶向 CAR-T 细胞在卵巢癌模型中的有效性和安全性  |  Monzo, HJ., et al. 2023. Mol Cancer Ther. 22: 1319-1331. PMID: 37486980
  8. 表皮生长因子受体-T790M突变的TKI耐药非小细胞肺癌中SSEA-4的表达增加。  |  Chen, NY., et al. 2024. Proc Natl Acad Sci U S A. 121: e2313397121. PMID: 38252815
  9. 开发皮下 SSEA3 或 SSEA4 阳性细胞捕获装置。  |  Nakayama, Y. and Iwai, R. 2024. Bioengineering (Basel). 11: PMID: 38927821
  10. 分离灵长类胚胎干细胞系。  |  Thomson, JA., et al. 1995. Proc Natl Acad Sci U S A. 92: 7844-8. PMID: 7544005
  11. 从普通狨猴（Callithrix jacchus）囊胚中提取的多能细胞系。  |  Thomson, JA., et al. 1996. Biol Reprod. 55: 254-9. PMID: 8828827
  12. 从培养的人类原始生殖细胞中提取多能干细胞。  |  Shamblott, MJ., et al. 1998. Proc Natl Acad Sci U S A. 95: 13726-31. PMID: 9811868

订购信息

产品名称产品编号规格价格数量收藏夹

SSEA-4 抗体 (813-70)

sc-21704
200 µg/ml
$316.00

SSEA-4 抗体 (813-70) AC

sc-21704 AC
500 µg/ml, 25% agarose
$416.00

SSEA-4 抗体 (813-70) Alexa Fluor® 488

sc-21704 AF488
200 µg/ml
$357.00

SSEA-4 抗体 (813-70) Alexa Fluor® 647

sc-21704 AF647
200 µg/ml
$357.00

SSEA-4 抗体 (813-70) FITC

sc-21704 FITC
200 µg/ml
$330.00

SSEA-4 抗体 (813-70) HRP

sc-21704 HRP
200 µg/ml
$316.00

SSEA-4 抗体 (813-70) PE

sc-21704 PE
200 µg/ml
$343.00

Can we do IF with another secondary antibody(Thermo; A32766; Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488) combined with this primary antibody (sc-21704) ?

Asked by: xixi
Thank you for your question.SSEA-4 Antibody (813-70) is a mouse monoclonal IgG3 κ Antibody.You can use a secondary antibody of anti mouse IgG (H+L) . Thank you.
Answered by: Jiajun Z
Date published: 2024-10-17

Is there any difference between two SSEA4 antibodies (sc-59368 and sc-21704)?

Asked by: Toshiaki_W
Thank you for your question. Both Anti-SSEA-4 Antibody (MC813): sc-59368 and Anti-SSEA-4 Antibody (813-70): sc-21704 are mouse monoclonal IgG3 κ antibodies that recognizes SSEA-4 and are recommended for IF and FCM. They recognize different epitopes on their target protein and as such may have different binding properties from one another.
Answered by: Tech Support Europe
Date published: 2021-08-16

I would like to detect SSEA-4 by flow cytometer. Does this antibody (sc-21704 PE) contain conjugated dye which would emit the step of incubation with secondary antibody? Which isotype control should I use for this antibody (sc-21704 PE)?

Asked by: arup
Thank you for your question. Yes, we offer this antibody directly conjugated to phycoerythrin (sc-21704 PE), which would allow for direct fluorescent detection without the use of a secondary antibody. SSEA-4 Antibody (813-70) is a mouse monoclonal IgG3. If you are using the PE conjugated primary antibody, the recommended isotype control is normal mouse IgG3-PE: sc-2869. A link is provided below as a reference. https://www.scbt.com/scbt/product/normal-mouse-igg3-pe
Answered by: Technical Support
Date published: 2024-10-16

Which negative control should be used when using the FITC conjugated SSEA-4 (813-70): sc-21704 in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using isotype specific normal mouse IgG3 conjugated to FITC, sc-2858
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 4 out of 5 by from Good for IF on frozen mouse brain sectionsI tested as free sample recently using fresh cut brain sections that was fixed with 4% PFA for 15 min. I got a strong IF signal with 1:50 dilution of SSEA-4.
Date published: 2022-02-23
Rated 4 out of 5 by from Good antibodyI used this antibody for quality check of hPSCs. It worked well.
Date published: 2021-02-16
Rated 5 out of 5 by from SSEA4We used it for immunofluorescence in human ESC and iPSC. It worked ok!
Date published: 2018-11-23
Rated 5 out of 5 by from ResultI use it for IF.My experiment material is human induced pluripotent stem cells,antibody dilution ratio is 1:50,but my experiment failed.Thank you for your provide samples, I'll try to find the reason and do it again.
Date published: 2017-09-06
Rated 4 out of 5 by from Great productBoth fcm and ihc results were very strong; pleased
Date published: 2017-01-02
Rated 5 out of 5 by from Used for Flow Cytometry with human ESCsUsed for Flow Cytometry with human ESCs. -SCBT Publication Review
Date published: 2015-05-17
Rated 5 out of 5 by from Published IF data using hESC lines HUESPublished IF data using hESC lines HUES-5 (H5) & WA09(H9). -SCBT Publication Review
Date published: 2015-05-15
Rated 5 out of 5 by from ICC: human skin biopsiesICC: human skin biopsies. -SCBT Publication Review
Date published: 2015-04-21
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SSEA-4 抗体 (813-70) is rated 4.7 out of 5 by 10.
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