Date published: 2025-9-9

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SSEA-3 抗体 (631): sc-21703

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说明书
  • SSEA-3 抗体 631 是大鼠单克隆 IgM,SSEA-3抗体, 在21篇文献中引用,规格为200 µg/ml
  • 针对4-8细胞期mouse胚胎
  • 推荐用于 mouse, rat 和 human 来源的SSEA-3 IF 和 FCM检测
  • 另提供琼脂糖偶联抗体,规格为琼脂糖装,可用于IP实验;HRP 偶联抗体用于WB, IHC(P)和ELISA实验;以及藻红蛋白、FITC、Alexa Fluor® 488Alexa Fluor® 647 偶联抗体,规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取SSEA-3 (631): sc-21703免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成SSEA-3 抗体 (631)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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SSEA-3 抗体 (631) 是一种鼠单克隆 IgM 抗体,可通过免疫荧光 (IF) 和流式细胞术 (FCM) 检测小鼠、大鼠和人类样本中的 SSEA-3。抗SSEA-3抗体(631)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。SSEA-3在鉴定和表征多能干细胞方面发挥着至关重要的作用,是未分化胚胎干细胞和胚胎癌细胞的关键标记物。SSEA-3主要位于细胞表面,有助于维持干细胞的未分化状态,在保持向各种细胞类型分化的同时促进增殖。SSEA-3的表达对于分离和研究干细胞至关重要,它能够区分未分化和已分化细胞,支持发育生物学和再生医学研究。SSEA-3还参与细胞间相互作用和信号传导通路,对干细胞的维持和分化至关重要,因此SSEA-3成为了解干细胞生物学和潜在治疗应用的重要靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SSEA-3 抗体 (631) 参考文献:

  1. 人类胚胎干细胞的表面抗原:培养分化过程中的变化。  |  Draper, JS., et al. 2002. J Anat. 200: 249-58. PMID: 12033729
  2. SSEA-3是结直肠癌中一种新型的扩增癌细胞表面标记物。  |  Suzuki, Y., et al. 2013. Int J Oncol. 42: 161-7. PMID: 23175153
  3. 人类胚胎癌细胞及其培养分化。  |  Andrews, PW., et al. 1987. Int J Androl. 10: 95-104. PMID: 2438234
  4. 开发荧光标记的 SSEA-3、SSEA-4 和 Globo-H 糖磷脂,用于阐明细胞膜中的分子相互作用。  |  Asano, S., et al. 2019. Int J Mol Sci. 20: PMID: 31817926
  5. 糖磷脂SSEA-3和FGF2在多线性分化应激持久细胞(MUSE)的干性和系承中的作用  |  Aprile, D., et al. 2023. Cell Prolif. 56: e13345. PMID: 36225120
  6. 阶段特异性胚胎抗原(SSEA-3 和 -4)是从人类畸胎瘤细胞中分离出来的一种独特的球状神经节苷脂的表位。  |  Kannagi, R., et al. 1983. EMBO J. 2: 2355-61. PMID: 6141938
  7. 人类睾丸生殖细胞肿瘤中鼠阶段特异性胚胎抗原的免疫组织化学定位。  |  Damjanov, I., et al. 1982. Am J Pathol. 108: 225-30. PMID: 6152898
  8. 人类畸胎瘤中新的球状糖磷脂与针对发育调节抗原--阶段特异性胚胎抗原 3 的单克隆抗体发生反应。  |  Kannagi, R., et al. 1983. J Biol Chem. 258: 8934-42. PMID: 6863318
  9. 克隆人类胚胎癌细胞系的细胞表面抗原:培养过程中的形态和抗原分化。  |  Andrews, PW., et al. 1982. Int J Cancer. 29: 523-31. PMID: 7095898
  10. 分离灵长类胚胎干细胞系。  |  Thomson, JA., et al. 1995. Proc Natl Acad Sci U S A. 92: 7844-8. PMID: 7544005
  11. 从普通狨猴(Callithrix jacchus)囊胚中提取的多能细胞系。  |  Thomson, JA., et al. 1996. Biol Reprod. 55: 254-9. PMID: 8828827
  12. 从培养的人类原始生殖细胞中提取多能干细胞。  |  Shamblott, MJ., et al. 1998. Proc Natl Acad Sci U S A. 95: 13726-31. PMID: 9811868

订购信息

产品名称产品编号规格价格数量收藏夹

SSEA-3 抗体 (631)

sc-21703
200 µg/ml
$316.00

SSEA-3 抗体 (631) AC

sc-21703 AC
500 µg/ml, 25% agarose
$416.00

SSEA-3 抗体 (631) Alexa Fluor® 488

sc-21703 AF488
200 µg/ml
$357.00

SSEA-3 抗体 (631) Alexa Fluor® 647

sc-21703 AF647
200 µg/ml
$357.00

SSEA-3 抗体 (631) FITC

sc-21703 FITC
200 µg/ml
$330.00

SSEA-3 抗体 (631) HRP

sc-21703 HRP
200 µg/ml
$316.00

SSEA-3 抗体 (631) PE

sc-21703 PE
200 µg/ml
$343.00

Can this antibody be used to stain rat cells or tissue?

Asked by: AbPolly
Thank you for your question. Yes, this rat monoclonal antibody is recommended for use with rat samples. We recommend using a directly conjugated primary antibody. This antibody is available conjugated to HRP, FITC, PE, AF488 and AF647.
Answered by: Technical Support 7
Date published: 2017-02-03
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Rated 5 out of 5 by from good qualityThis antibody is widely used for IF, it is very good quality (1:200 dilution).We tried different suppliers, this antibody is the best.
Date published: 2017-09-02
Rated 5 out of 5 by from western blotThis antibody been diluted (1:1000) in 3% BSA/TBS-T and shows high specificity to target protein(only weak unspecific bands were detected when 75ug/lane of cell lysate was loaded.
Date published: 2017-07-28
Rated 5 out of 5 by from strong stainingIt worked very well for human cell lines. I used 1/400 concentration for IF, got strong staining.
Date published: 2017-06-01
Rated 5 out of 5 by from single bandStrong single band for WB in mouse brain tissue ,good specificity.
Date published: 2017-05-31
Rated 5 out of 5 by from strong signalSSEA-3 antibody used for IF staining , I got strong signal at 1:400.
Date published: 2017-02-24
Rated 5 out of 5 by from Worked well for Flow Cytometry studies withWorked well for Flow Cytometry studies with human ESCs. -SCBT Publication Review
Date published: 2015-03-28
Rated 5 out of 5 by from Published IF results using PFA fixed NTERAPublished IF results using PFA fixed NTERA-2 cl.D1 (NT2) cells. -SCBT Publication Review
Date published: 2015-03-25
Rated 5 out of 5 by from Used for IHC and ELISA with NT2 cellsUsed for IHC and ELISA with NT2 cells. -SCBT Publication Review
Date published: 2015-03-18
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SSEA-3 抗体 (631) is rated 5.0 out of 5 by 10.
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