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SSEA-1 Antibody (480) - Immunohistochemistry Immunofluorescence - Image 8000
SSEA-1 抗体 (480): sc-21702. 福尔马林固定, 石蜡包埋的人肝组织的免疫过氧化物酶染色 (A) 和甲醇固定的 F9 细胞的免疫荧光染色 (B), 显示膜定位.
SSEA-1 抗体 (480): sc-21702. 福尔马林固定, 石蜡包埋的人肝组织的免疫过氧化物酶染色 (A) 和甲醇固定的 F9 细胞的免疫荧光染色 (B), 显示膜定位.

SSEA-1 抗体 (480): sc-21702

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说明书
  • SSEA-1 抗体 480 是小鼠单克隆 IgM κ，SSEA-1抗体, 在187篇文献中引用，规格为200 µg/ml
  • 针对X射线照射的F9畸胎瘤干细胞
  • 推荐用于 mouse, rat 和 human 来源的SSEA-1 WB, IP, IF, IHC(P) 和 FCM检测
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 关于如何获取SSEA-1 (480): sc-21702的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成SSEA-1 抗体 (480)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
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研究信息

関連項目

此外 SSEA 抗体 包括 SSEA-1, SSEA-3和SSEA-4

SSEA-1抗体(480)是一种IgM κ小鼠单克隆SSEA-1抗体（也称为干细胞标记SSEA1抗体、糖鞘脂表位特异性抗体、多能性表面标记SSEA1抗体、Sialyl LewisX (sLeX)抗体、分化簇15s (CD15s)抗体或四糖碳水化合物表位特异性抗体），可通过WB、IP、IF、IHC(P)和FCM检测小鼠、大鼠和人源的SSEA-1蛋白。SSEA-1抗体(480)既可作为非偶联抗SSEA-1抗体形式，也可作为多种偶联形式的抗SSEA-1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。胚胎干细胞具有在体内保持未分化状态和无限增殖的能力，同时保持分化为所有三个胚胎胚层的衍生物的潜力。未分化的人胚胎癌（EC）细胞是畸胎瘤的干细胞，其特征是表达阶段特异性胚胎抗原SSEA-1和SSEA-3、TRA-2-39、TRA-2-54和高分子量糖蛋白TRA-1-60和TRA-1-81。此外，SSEA-1、SSEA-3和SSEA-4是表征胚胎干细胞（ES）和胚胎生殖（EG）细胞的标记。具体来说，来自人类ES细胞系H7的未分化细胞表达SSEA-3、SSEA-4、TRA-1-60和TRA-1-81，但不表达SSEA-1。干扰素诱导EC细胞表达SSEA-3和SSEA-4，而不抑制其生长或诱导其分化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SSEA-1 抗体 (480) 参考文献:

  1. 人类胚胎干细胞的表面抗原：培养分化过程中的变化。  |  Draper, JS., et al. 2002. J Anat. 200: 249-58. PMID: 12033729
  2. 人类胚胎癌细胞及其培养分化。  |  Andrews, PW., et al. 1987. Int J Androl. 10: 95-104. PMID: 2438234
  3. 唾液酸Lewis X/A和细胞角蛋白在三阴性乳腺癌中的相互作用。  |  Xu, J., et al. 2016. Endocr Pathol. 27: 271-275. PMID: 27550342
  4. 循环SSEA-1+干细胞介导的组织修复在过敏性气道炎症中的作用。  |  Trinchera, M., et al. 2017. Biology (Basel). 6: PMID: 28241499
  5. CD15-(Le(X))相关碳水化合物在中性粒细胞粘附中的作用。  |  Luque-Molina, I., et al. 2017. Stem Cells. 35: 2417-2429. PMID: 28869691
  6. SSEA-1与乳腺癌的侵袭性表型相关。  |  Szlasa, W., et al. 2022. Cancers (Basel). 14: PMID: 35565333
  7. Lewis x抗原与头颈部鳞状细胞癌的存活率相关。  |  Guan, M., et al. 2022. J Virol. 96: e0134422. PMID: 36125302
  8. 治疗期间持续HIV转录的细胞富含唾液酸-LewisX糖抗原。  |  Pascoal, C., et al. 2023. Cancers (Basel). 15: PMID: 36765690
  9. 胰腺癌组织中MUC5AC和MUC1粘蛋白的唾液酸-LewisX分析。  |  Kohler, KT., et al. 2023. J Histochem Cytochem. 71: 423-430. PMID: 37477396
  10. 分离灵长类胚胎干细胞系。  |  Thomson, JA., et al. 1995. Proc Natl Acad Sci U S A. 92: 7844-8. PMID: 7544005
  11. 从普通狨猴（Callithrix jacchus）囊胚中提取的多能细胞系。  |  Thomson, JA., et al. 1996. Biol Reprod. 55: 254-9. PMID: 8828827
  12. 从培养的人类原始生殖细胞中提取多能干细胞。  |  Shamblott, MJ., et al. 1998. Proc Natl Acad Sci U S A. 95: 13726-31. PMID: 9811868

订购信息

产品名称产品编号规格价格数量收藏夹

SSEA-1 抗体 (480)

sc-21702
200 µg/ml
$316.00

SSEA-1 抗体 (480) AC

sc-21702 AC
500 µg/ml, 25% agarose
$416.00

SSEA-1 抗体 (480) Alexa Fluor® 405

sc-21702 AF405
200 µg/ml
$357.00

SSEA-1 抗体 (480) Alexa Fluor® 488

sc-21702 AF488
200 µg/ml
$357.00

SSEA-1 抗体 (480) Alexa Fluor® 647

sc-21702 AF647
200 µg/ml
$357.00

SSEA-1 抗体 (480) FITC

sc-21702 FITC
200 µg/ml
$330.00

SSEA-1 抗体 (480) HRP

sc-21702 HRP
200 µg/ml
$316.00

SSEA-1 抗体 (480) PE

sc-21702 PE
200 µg/ml
$343.00

What exactly does it mean that this antibody is "monoclonal mouse IgM κ (kappa light chain)" antibody? Is there no heavy chain present in this antibody molecule? Can standard anti-mouse IgM secondary antibodies recognize this IgM κ antibody?

Asked by: TSaric
Thank you for your question. SSEA-1 (480): sc-21702 is a complete monomeric IgM antibody with heavy and light chains. It can be detected either by regular anti-mouse IgM antibody or by using one of our recommended κ light chain-specific secondary binding proteins, such as m-IgGκ BP-HRP sc-516102, or one of our CruzFluor conjugated κ binding proteins. You can find all available options by following this link: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

What secondary antibody may I use for WB? The antibody sc-21702 is a IgM Light chain.

Asked by: pablo
Thank you for your question. We would suggest using our mouse IgG kappa binding protein secondary reagent (sc-516102) for an IgM antibody. If you would like to try a sample of the reagent, please contact your local distributor. You can find their contact information here: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-06-20

What secondary reagent should I use if I want to perform IF studies in mice tissue samples?

Asked by: Trav11
In this case, we recommend using one of our directly conjugated mouse monoclonal primary antibodies. Currently, SSEA-1 Antibody (480): sc-21702 is available directly conjugated to HRP (sc-21702 HRP), phycoerythrin (sc-21702 PE), fluorescein (sc-21702 FITC), Alexa Fluor® 488 (sc-21702 AF488), Alexa Fluor® 647 (sc-21702 AF647), PerCP (sc-21702 PerCP) or Alexa Fluor® 405 (sc-21702 AF405). If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2016-12-30
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Rated 5 out of 5 by from Parfait !Antibody tested on paraffin section (mouse embryonic gonad 12.5 days post coitum). This antibody perfectly detects germ cells. The membrane signal is perfect and there is no background noise. Conditions:   - Demasking with Pt Link from Dako (pH6 or pH9)   - Dilution 1/500 and 1/200 works well   - Secondary antibody used: Alexa anti-mouse 647
Date published: 2018-06-15
Rated 5 out of 5 by from Beautiful stainingWonderful staining seen in IHC and IF with both methanol fixation and FFPE sections.
Date published: 2017-01-12
Rated 5 out of 5 by from Worked well with mIPS cells by IFTo detect the expression of SSEA-1 in miPS cells by IF, I bought this antibody. The cells were incubated with sc-21702 at a 1/500 dilution overnight at +4°C, and found this antibody worked well.
Date published: 2016-12-06
Rated 5 out of 5 by from 抗体效价很高,使用效果很好,使用干细胞染色分群很开几个月前购买的该抗体，作为流式细胞分选和分析用。使用该抗体进行多能干细胞染色效果很好用，分群比较开。
Date published: 2016-11-17
Rated 5 out of 5 by from It's woeked well in IFIt's woeked well in IF
Date published: 2015-11-17
Rated 5 out of 5 by from Used for studying adult stem cell/pluripotencyUsed for studying adult stem cell/pluripotency
Date published: 2015-05-28
Rated 5 out of 5 by from Worked for western blotting in MEF cellsWorked for western blotting in MEF cells. -SCBT Publication Review
Date published: 2015-04-12
Rated 5 out of 5 by from Published immunofluorescence data using murinePublished immunofluorescence data using murine ES: embryonic stem cells. -SCBT Publication Review
Date published: 2015-04-05
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SSEA-1 抗体 (480) is rated 5.0 out of 5 by 11.
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