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SREBP-1 抗体 (F-10): sc-365514

4.7(3)
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说明书
  • SREBP-1抗体(F-10)是小鼠单克隆IgG3 κ, 在9篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的SREBP-1的C-terminus的氨基酸1119-1147之间
  • 推荐用于 mouse, rat 和 human 来源的SREBP-1 WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应,包括: 和 canine, bovine and porcine
  • 看 SREBP-1 (A-4): sc-365513,可查看有标记的 SREBP-1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取SREBP-1 (F-10): sc-365514免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成SREBP-1 抗体 (F-10)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

SREBP-1抗体(F-10)是一种IgG3 κ小鼠单克隆SREBP-1抗体(也称为SREBP-1抗体或SREBF1抗体),它能通过蛋白质印迹法(WB)、免疫沉淀法(IP)、免疫荧光法(IF)、免疫组化法(IHC(P))和酶联免疫吸附测定法(ELISA)检测来自小鼠、大鼠和人类的SREBP-1。SREBP-1抗体(F-10)为非偶联形式的抗SREBP-1抗体。低密度脂蛋白(LDL)受体介导携带胆固醇的脂蛋白的内吞摄取,从而控制细胞和血浆中的胆固醇水平。LDL受体基因的转录受5'侧翼区一个由十个碱基对组成的序列控制,该序列被称为固醇调节元件1(SRE-1)。当细胞固醇储存耗尽时,该元件被激活,基因开始转录,细胞对LDL的摄取增加。已经发现了一组SRE结合蛋白(SREBPs),包括两个基本螺旋-环-螺旋亮氨酸拉链(bHLH-Zip)转录因子,分别命名为SREBP-1和SREBP-2。SREBP-1(也称为ADD1,即脂肪细胞决定和分化因子)以前体形式合成,附着在核膜和内质网上。在固醇耗尽的细胞中,膜结合前体被切割,生成一个可溶的NH2-末端片段,该片段易位至细胞核以激活转录。固醇抑制SREBP-1的裂解。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

SREBP-1 抗体 (F-10)

sc-365514
200 µg/ml
$316.00

SREBP-1 (F-10) 中和勝肽

sc-365514 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-365514 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of SREBP-1 expression in A549 and Jurkat whole cell lysates.
Date published: 2017-02-07
Rated 4 out of 5 by from An increase in signal was observed via WesternAn increase in signal was observed via Western Blot when comparing human SREBP-1 transfected 293T cells with those that have not been transfected to over express the protein of interest. A large increase in signal was observed. Some background and non-specific binding observed as well. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Beautiful immunofluorescence cytoBeautiful immunofluorescence cyto-plasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-03
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SREBP-1 抗体 (F-10) is rated 4.7 out of 5 by 3.
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