Date published: 2025-9-7
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SREBP-1 Antibody (2A4) - Western Blotting - Image 396264
SREBP-1 抗体 (2A4) Alexa Fluor® 680: sc-13551 AF680. 直接近红外 Western 印迹分析 A549 全细胞裂解液 (A) 和 HeLa 核提取物 (B) 中 SREBP-1 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.
SREBP-1 抗体 (2A4) Alexa Fluor® 680: sc-13551 AF680. 直接近红外 Western 印迹分析 A549 全细胞裂解液 (A) 和 HeLa 核提取物 (B) 中 SREBP-1 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.

SREBP-1 抗体 (2A4): sc-13551

4.6(30)
寫評論提問

说明书
  • SREBP-1抗体(2A4)是小鼠单克隆IgG1 κ, 在381篇文献中引用，规格为200 µg/ml
  • 免疫human物种的SREBP-1a的氨基酸301-407
  • 抗-SREBP-1 抗体 (2A4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和hamster 来源的 SREBP-1
  • SREBP-1抗体（2A4）可与琼脂糖结合用于IP
  • 还可偶联Alexa Fluor® 594 用于IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13551 X, 200 µg/0.1 ml)
  • 看 SREBP-1 (A-4): sc-365513，可查看有标记的 SREBP-1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取SREBP-1 (2A4): sc-13551的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 SREBP-1 抗体 (2A4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与SREBP-1 抗体 (2A4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外SREBP抗体，包括SREBP-2

SREBP-1抗体（2A4）是一种IgG1κ小鼠单克隆SREBP-1抗体（也称为SREBF1抗体），可通过WB、IP、IF和IHC（P）检测来自小鼠、大鼠和人类的SREBP-1。SREBP-1抗体（2A4）既可以是非偶联的，也可以是多种偶联形式的。低密度脂蛋白（LDL）受体介导胆固醇载脂蛋白的内吞吸收，从而控制细胞和血浆中的胆固醇水平。LDL受体基因的转录受5'翼区内的十个碱基对序列（称为醇调节元件1（SRE-1））的控制。当细胞醇储备耗尽时，该元件被激活，基因被转录，细胞对LDL的吸收增加。已经确定了一组SRE结合蛋白（SREBPs），包括两种基质-环-螺旋-茎叶-苦瓜蛋白（bHLH-Zip）转录因子，分别称为SREBP-1和SREBP-2。SREBP-1（也称为ADD1，即脂肪细胞决定和分化因子）以前体的形式合成，连接到核膜和内质网。在醇耗尽的细胞中，膜结合的前体被切割以产生可溶性的NH2末端片段，该片段转移到核内以激活转录。醇可抑制SREBP-1的切割。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SREBP-1 抗体 (2A4) 参考文献:

  1. 人皮脂腺和培养的皮脂细胞中生脂因子 galectin-12, resistin, SREBP-1 和 SCD 的表达。  |  Harrison, WJ., et al. 2007. J Invest Dermatol. 127: 1309-17. PMID: 17363919
  2. HNF1α失活会促进人肝细胞腺瘤的脂肪生成，而不依赖于SREBP-1和碳水化合物反应元件结合蛋白（ChREBP）的激活。  |  Rebouissou, S., et al. 2007. J Biol Chem. 282: 14437-46. PMID: 17379603
  3. 调节甲羟戊酸途径。  |  Goldstein, JL. and Brown, MS. 1990. Nature. 343: 425-30. PMID: 1967820
  4. 鉴定低密度脂蛋白受体基因中固醇调节元件增强子活性的核苷酸。  |  Smith, JR., et al. 1990. J Biol Chem. 265: 2306-10. PMID: 2298751
  5. 靶向SREBP-1驱动的脂质代谢治疗癌症。  |  Guo, D., et al. 2014. Curr Pharm Des. 20: 2619-26. PMID: 23859617
  6. 受体介导的胆固醇平衡途径。  |  Brown, MS. and Goldstein, JL. 1986. Science. 232: 34-47. PMID: 3513311
  7. 靶向SREBP-1/Hsa-Mir-497/SCAP/FASN肿瘤代谢轴抑制非小细胞肺癌细胞的癌干细胞样和耐药表型。  |  Tiong, TY., et al. 2022. Int J Mol Sci. 23: PMID: 35806291
  8. SREBP-1介导的脂质生成赋予对铁死亡的抗性并改善内皮损伤。  |  Wang, X., et al. 2024. FASEB J. 38: e23806. PMID: 38970404
  9. SREBP-2是第二种碱性-螺旋-环-螺旋-亮氨酸拉链蛋白，通过与固醇调节元件结合刺激转录。  |  Hua, X., et al. 1993. Proc Natl Acad Sci U S A. 90: 11603-7. PMID: 7903453
  10. SREBP-1，一种通过固醇调节蛋白水解释放的膜结合转录因子。  |  Wang, X., et al. 1994. Cell. 77: 53-62. PMID: 8156598
  11. 结合低密度脂蛋白受体启动子固醇调节元件的核蛋白。II.纯化和表征。  |  Wang, X., et al. 1993. J Biol Chem. 268: 14497-504. PMID: 8314806
  12. 结合低密度脂蛋白受体启动子固醇调节元件的核蛋白。I. 蛋白质的鉴定及其靶核苷酸序列的描述。  |  Briggs, MR., et al. 1993. J Biol Chem. 268: 14490-6. PMID: 8390995

订购信息

产品名称产品编号规格价格数量收藏夹

SREBP-1 抗体 (2A4)

sc-13551
200 µg/ml
$316.00

SREBP-1 (2A4): m-IgG Fc BP-HRP 套装

sc-525476
200 µg Ab; 10 µg BP
$354.00

SREBP-1 抗体 (2A4) X

sc-13551 X
200 µg/0.1 ml
$316.00

SREBP-1 抗体 (2A4) AC

sc-13551 AC
500 µg/ml, 25% agarose
$416.00

SREBP-1 抗体 (2A4) Alexa Fluor® 546

sc-13551 AF546
200 µg/ml
$357.00

SREBP-1 抗体 (2A4) Alexa Fluor® 594

sc-13551 AF594
200 µg/ml
$357.00

SREBP-1 抗体 (2A4) Alexa Fluor® 680

sc-13551 AF680
200 µg/ml
$357.00

SREBP-1 抗体 (2A4) Alexa Fluor® 790

sc-13551 AF790
200 µg/ml
$357.00

I used the sc-13551 antibody to detect SREBP1 expression in the kidneys and livers of diabetic rats, with a sample loading amount of 64 μg. I detected the protein in the liver but not in the kidney. How should I adjust the experiment?

Asked by: Vinco1076
Thanks for your question. SREBP-1 is expressed predominantly in brown adipose tissue.The positive results in rat liver indicate that there is no problem with your experimental protocol and antibody. It is recommended that you refer to the literature to confirm the expression of this protein in rat kidney tissue. 感谢您的提问。SREBP-1主要表达于棕色脂肪组织。在大鼠的肝脏中获得阳性，说明您的实验流程以及抗体是没有问题的，建议您针对性的查阅文献确认大鼠肾脏组织中该蛋白的表达情况。
Answered by: Yanru Tao
Date published: 2024-11-07

Can this antibody detect both precusor and mature SREBP1 at 68kd?

Asked by: ssk1234
Thank you for your question. It would be helpful if you could email us directly at asia@scbio.cn. So we can have a more interactive discussion about this and other related issues.
Answered by: Zhanglong
Date published: 2024-04-24

Can this antibody detect both precusor and mature SREBP1 at 68kd?

Asked by: feng yin
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2024-08-16

Hi, has anybody used this antibody for immuno-fluorescent staining on mouse liver tissue? 

Asked by: Daniella1616
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2024-08-16

Hi There, I am using this Ab "SREBP-1 Antibody (2A4): sc-13551" for rat brain samples in WB, but alwayes I am getting non specific bands besids the desired bands! any recommendations to get clear and neat bands. Regards

Asked by: Mustafa00
Thank you for your question. Please contact your local distributor or Technical Service directly for assistance regarding your WB results, we are happy to help troubleshoot this with you.
Answered by: Tech Service
Date published: 2022-12-10

What is the difference between sc-13551 and sc-13551X? Can the sc-13551: mouse monoclonal SREBP-1 Antibody (2A4) be used for Supershift Assay?

Asked by: Taro
Thank you for your question. The only difference between these two products is that the “X” form is 10x more concentrated and vialed in a buffer without stabilizing protein (gelatin). This TransCruz reagent “X” is for Gel Supershift (EMSA) and ChIP applications. sc-13551 X, 200 µg/0.1 ml
Answered by: Technical Support
Date published: 2023-11-12

Hi, I would like to use the antibody in Drosophila tissue, Do you have any records or would it be possible to have a sample for testing? thank you very much

Asked by: MaryV
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2021-12-08

Hi, We need SREBP-1 antibody to use it in Atlantic salmon tissues. Could you please tell us about feasibility? BR

Asked by: Mmmmj
Thank you for your interest in our SREBP-1 antibodies. We do not have any antibodies currently validated for use in Atlantic salmon tissues, however we are happy to arrange samples for you to test. Please contact our Europe Technical Service team by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com to arrange the samples.
Answered by: Technical Service
Date published: 2017-07-17
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Rated 4 out of 5 by from Good performance in Western BlotThis antibody performs well in Western Blot using total protein lysates from U87-MG cells. A single, distinct band was observed at approximately 130 kDa.
Date published: 2025-05-05
Rated 4 out of 5 by from Good performance in Western BlotThis antibody performs well in Western Blot using total protein lysates from U87-MG cells. A single, distinct band was observed at approximately 130 kDa.
Date published: 2025-05-05
Rated 5 out of 5 by from goodThis antibody works great in liver of mice and Human hepatocellular carcinoma cell line (Huh7).
Date published: 2024-08-09
Rated 5 out of 5 by from Good antibodyThis antibody works great in skeletal muscle of mice
Date published: 2024-08-09
Rated 5 out of 5 by from Good for western blotThis product works well in our western blot, shows clear bands with very good quality!
Date published: 2023-11-22
Rated 4 out of 5 by from Goodsc-13551 works good in rat skeletal muscle samples.
Date published: 2023-05-10
Rated 5 out of 5 by from goodthis antibody works well in skeletal muscle of mice
Date published: 2022-10-01
Rated 1 out of 5 by from I didn't see any band of SREBP1I bought this 2 weeks ago. I tried several times of this antibody, but I can't get any band of that.
Date published: 2021-03-26
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SREBP-1抗体 (2A4) is rated 4.6 out of 5 by 32.
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