Date published: 2026-10-19
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Scrib Antibody (D-8) - Western Blotting - Image 353269
Scrib 抗体 (D-8): sc-55532. Hep G2 全细胞裂解液中 Scrib 表达的 Western 印迹分析.
Scrib 抗体 (D-8): sc-55532. Hep G2 全细胞裂解液中 Scrib 表达的 Western 印迹分析.

Scrib 抗体 (D-8): sc-55532

4.8(4)
寫評論提問
说明书
  • Scrib抗体(D-8)是小鼠单克隆IgM κ, 在3篇文献中引用，规格为200 µg/ml
  • 与氨基酸1331-1630 deletion 1444-1504（缺失1444-1504）在Scrib的human起源的C-terminus处进行比对
  • 推荐用于 human 来源的Scrib WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 Scrib (C-6): sc-55543，可查看有标记的 Scrib 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成Scrib 抗体 (D-8)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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関連項目

黑腹果蝇的基因根据其所编码的蛋白质类型被分为六大类，包括细胞内信号蛋白、跨膜蛋白、RNA结合蛋白、分泌因子、转录调控因子（如基本螺旋-环-螺旋、含同源域、含锌指和与染色质相关）以及其他功能蛋白。果蝇的形态发生和细胞分化需要对细胞分裂进行精确控制。大盘状（Dlg）、Scribble（Scrib）和致死性巨幼细胞（Lgl）肿瘤抑制蛋白调控神经母细胞不对称细胞分裂的多个方面。Dlg/Scrib/Lgl蛋白在前期/中期显示顶端皮质富集，并具有均匀的皮质分布。编码多PDZ（PSD-95、Discs-large和ZO-1）和富含亮氨酸重复蛋白Scrib的基因突变会导致细胞形态异常和胚胎上皮单层组织的丧失。人类同源物hScrib定位于脊椎动物紧密连接处，起着正确放置粘附连接的作用。Scrib的PDZ结构域被预测与蛋白质C末端的共识S/TXV结合。PDZ结构域蛋白已涉及蛋白质运输途径的多个不同位点，这表明Scrib对于几种上皮决定因素的定位是必需的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Scrib 抗体 (D-8) 参考文献:

  1. Scrib调节HGF介导的上皮形态发生，并通过Sgt1-HSP90稳定。  |  Eastburn, DJ., et al. 2012. J Cell Sci. 125: 4147-57. PMID: 22623728
  2. 极性蛋白SCRIB可抑制小鼠动脉粥样硬化的发展。  |  Schürmann, C., et al. 2019. Cardiovasc Res. 115: 1963-1974. PMID: 30949676
  3. 肿瘤抑制因子SCRIB是Wnt/β-Catenin信号传导途径的负调控因子。  |  Daulat, AM., et al. 2019. Proteomics. 19: e1800487. PMID: 31513346
  4. Scrib和Dlg1极性蛋白调节人类树突状细胞中的抗原呈递。  |  Barreda, D., et al. 2020. J Leukoc Biol. 108: 883-893. PMID: 32293058
  5. 成纤维细胞中低水平的SCRIB表达促进肺癌细胞的侵袭。  |  Wang, Y., et al. 2020. Life Sci. 256: 117955. PMID: 32534038
  6. SCRIB通过靶向大肠癌中的Hippo/YAP信号传导促进增殖和转移。  |  Shen, H., et al. 2021. Front Cell Dev Biol. 9: 656359. PMID: 33937255
  7. 极性蛋白SCRIB与SLC3A2相互作用，调节ER+乳腺癌的增殖和他莫昔芬抗性。  |  Saito, Y., et al. 2022. Commun Biol. 5: 403. PMID: 35501367
  8. 小鼠神经元特异性缺失Scrib导致神经解剖和运动缺陷。  |  Ezan, J., et al. 2022. Front Genet. 13: 872700. PMID: 35692812
  9. SCRIB在细胞分化过程中控制顶端收缩。  |  Boëda, B., et al. 2023. J Cell Biol. 222: PMID: 37930352
  10. 为悲伤的孩子讲故事。  |  Olive, J. 1996. Am J Hosp Palliat Care. 13: 19-20. PMID: 8716323

订购信息

产品名称产品编号规格价格数量收藏夹

Scrib 抗体 (D-8)

sc-55532
200 µg/ml
$322.00

Which secondary antibody should we use since it is IgM?

Asked by: Santa
Thank you for your question. Scrib Antibody (D-8): sc-55532 is a mouse monoclonal IgM κ antibody that can be detected using m-IgGκ BP-HRP: sc-516102 or m-IgGκ BP-CFL 488: sc-516176 or any other kappa light chain binding protein.
Answered by: Tech Support Europe
Date published: 2024-03-28

Does it work on Scrib of mouse origin?

Asked by: Matrix
Thank you for your question. We have not yet tested our Scrib Antibody (D-8): sc-55532 for reactivity with mouse samples, so we do not know if it will work for that species. The antibody is raised against a portion of the human sequence that does share significant sequence identity with the mouse protein, so based on sequence analysis, there is a good possibility it will cross-react with mouse Scrib.
Answered by: Technical Support
Date published: 2017-05-30

I am using Scrib (D-8): sc-55532 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by Trav11 from High Quality ProductThe Scrib Antibody (D-8): sc-55532 works very well for IHC applications with formalin fixed, paraffin-embedded tissue sections, showing staining pattern with cytoplasmic localization.
Date published: 2017-02-07
Rated 4 out of 5 by SS from Strong bands seen in WB using HeLa and JurkatStrong bands seen in WB using HeLa and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-13
Rated 5 out of 5 by TS from WB and IHC publishable data with human tumorsWB and IHC publishable data with human tumors and tumor cell lines representative of malignant mesothelioma(MSTO-211H) and cancers of the colon (LoVo and SW1116), breast (MCF-7, MDA-MB231, and SKBR3),lung (A549), stomach (MKN45 and KATO-III), uterus(Hela), prostate (DU145), and liver (HepG2) - SCBT Publication Review
Date published: 2015-02-06
Rated 5 out of 5 by HF from Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
Date published: 2014-12-21
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Scrib 抗体 (D-8) is rated 4.8 out of 5 by 4.
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