SAP 114 Antibody (H-10): sc-515804
- SAP 114 Antibody (H-10) is a mouse monoclonal IgG2b κ SAP 114 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 25-44 near the N-terminus of SAP 114 of human origin
- SAP 114 Antibody (H-10) is recommended for detection of SAP 114 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SAP 114 Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-515804 X, 200 µg/0.1 ml)
- m-IgGκ BP-HRP is the preferred secondary detection reagent for SAP 114 Antibody (H-10) for WB applications. This reagent is now offered in a bundle with SAP 114 Antibody (H-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
SAP 114 Antibody (H-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects SAP 114 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SAP 114 (H-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SAP 114, also known as spliceosome-associated protein 114, PRP21, PRPF21, SF3A120, or SF3A1, is a 793 amino acid nuclear protein that is ubiquitously expressed and belongs to the SURP protein family, characterized by two SURP motif repeats and one ubiquitin-like domain. SAP 114 plays a crucial role in pre-mRNA splicing as a subunit of the SF3A splicing factor complex, which is essential for binding U2 small nuclear ribonucleoprotein (snRNP) to the branchpoint sequence in pre-mRNA. The SF3A complex is vital for converting 15S U2 snRNP into the active 17S form, directly involved in pre-mRNA splicing events. SAP 114 serves as the first subunit of this complex, interacting with subunit 2 (SAP 62) and subunit 3 (SAP 61) through SURP motifs, thereby facilitating E complex assembly, which regulates cell cycle progression from G1 phase into S phase in mammalian cells. SAP 114′s ability to mediate these interactions underscores its importance in gene expression regulation and cellular function, making SAP 114 a valuable target for research in molecular biology and related fields.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SAP 114 Antibody (H-10) References:
- Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. | Das, R., et al. 2000. Mol Cell. 5: 779-87. PMID: 10882114
- A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site. | Will, CL., et al. 2001. EMBO J. 20: 4536-46. PMID: 11500380
- Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. | Jurica, MS., et al. 2002. RNA. 8: 426-39. PMID: 11991638
- Statistical modeling for selecting housekeeper genes. | Szabo, A., et al. 2004. Genome Biol. 5: R59. PMID: 15287981
- Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. | Rush, J., et al. 2005. Nat Biotechnol. 23: 94-101. PMID: 15592455
- An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells. | Bürckstümmer, T., et al. 2006. Nat Methods. 3: 1013-9. PMID: 17060908
- Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114. | Chiara, MD., et al. 1994. Proc Natl Acad Sci U S A. 91: 6403-7. PMID: 8022796
- Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells. | Seghezzi, W., et al. 1998. Mol Cell Biol. 18: 4526-36. PMID: 9671462
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAP 114 Antibody (H-10) | sc-515804 | 200 µg/ml | $322.00 | |||
SAP 114 Antibody (H-10): m-IgGκ BP-HRP Bundle | sc-525226 | 200 µg Ab, 40 µg BP | $361.00 | |||
SAP 114 Antibody (H-10) X | sc-515804 X | 200 µg/0.1 ml | $322.00 | |||
SAP 114 Antibody (H-10) AC | sc-515804 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SAP 114 Antibody (H-10) HRP | sc-515804 HRP | 200 µg/ml | $322.00 | |||
SAP 114 Antibody (H-10) FITC | sc-515804 FITC | 200 µg/ml | $336.00 | |||
SAP 114 Antibody (H-10) PE | sc-515804 PE | 200 µg/ml | $349.00 | |||
SAP 114 Antibody (H-10) Alexa Fluor® 488 | sc-515804 AF488 | 200 µg/ml | $364.00 | |||
SAP 114 Antibody (H-10) Alexa Fluor® 546 | sc-515804 AF546 | 200 µg/ml | $364.00 | |||
SAP 114 Antibody (H-10) Alexa Fluor® 594 | sc-515804 AF594 | 200 µg/ml | $364.00 | |||
SAP 114 Antibody (H-10) Alexa Fluor® 647 | sc-515804 AF647 | 200 µg/ml | $364.00 | |||
SAP 114 Antibody (H-10) Alexa Fluor® 680 | sc-515804 AF680 | 200 µg/ml | $364.00 | |||
SAP 114 Antibody (H-10) Alexa Fluor® 790 | sc-515804 AF790 | 200 µg/ml | $364.00 |