ROD Antibody (43-K): sc-81853

ROD Antibody (43-K) is a mouse monoclonal IgG2a kappa light chain antibody that detects ROD protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ROD antibody (43-K) is available as the non-conjugated form. ROD, also known as KNTC1 (Kinetochore-associated protein 1), plays a critical role in the mitotic cell cycle checkpoint, where ROD (43-K) is essential for the assembly of MAD1-MAD2 and Dynein-Dynactin complexes at kinetochores. ROD is highly expressed in the testis and exhibits a dynamic localization pattern throughout the cell cycle, initially found in the nucleus and cytoplasm during interphase, and then translocating to kinetochores and spindle fibers during anaphase. ROD (43-K) functions to ensure accurate chromosome segregation, as ROD interacts with ZW10, forming a stable association with the kinetochore, which is crucial for spindle assembly. This interaction helps target the microtubule motor cytoplasmic Dynein to kinetochores, thereby facilitating the movement of chromosomes during cell division. ROD′s ability to regulate these processes underscores ROD′s importance in maintaining genomic stability and preventing aneuploidy, making ROD a significant target for research in cell biology and cancer studies.

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ROD Antibody (43-K) References:

1. The rough deal protein is a new kinetochore component required for accurate chromosome segregation in Drosophila.  |  Scaërou, F., et al. 1999. J Cell Sci. 112 (Pt 21): 3757-68. PMID: 10523511
2. Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores.  |  Chan, GK., et al. 2000. Nat Cell Biol. 2: 944-7. PMID: 11146660
3. The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore.  |  Scaërou, F., et al. 2001. J Cell Sci. 114: 3103-14. PMID: 11590237
4. Kinetochore dynein: its dynamics and role in the transport of the Rough deal checkpoint protein.  |  Wojcik, E., et al. 2001. Nat Cell Biol. 3: 1001-7. PMID: 11715021
5. Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions.  |  Williams, BC., et al. 2003. Mol Biol Cell. 14: 1379-91. PMID: 12686595
6. In vivo dynamics of the rough deal checkpoint protein during Drosophila mitosis.  |  Basto, R., et al. 2004. Curr Biol. 14: 56-61. PMID: 14711415
7. rough deal: a gene required for proper mitotic segregation in Drosophila.  |  Karess, RE. and Glover, DM. 1989. J Cell Biol. 109: 2951-61. PMID: 2512302

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ROD Antibody (43-K) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-ROD Antibody (43-K) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ROD Antibody (43-K) Specifications

Alternate Product Name: ROD

Product Clone Name: 43-K

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 2100-2210

Epitope Target: ROD

Epitope Species: human

Product Reactivity/Detection: ROD

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for ROD Antibody (43-K): N/A