Date published: 2025-9-7

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RNase HII-A 抗体 (G-10): sc-515475

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说明书
  • RNase HII-A 抗体 G-10 是小鼠单克隆 IgG1 κ,RNase HII-A抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长RNase HII-A的氨基酸1-299
  • RNase HII-A 抗体 (G-10) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 RNase HII-A
  • 抗RNase HII-A抗体(G-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RNase HII-A (G-10): sc-515475免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是RNase HII-A Antibody (G-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与RNase HII-A Antibody (G-10) 打包提供(请参阅下面的订购信息)。

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関連項目

RNase HII-A抗体(G-10)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测人类来源的RNase HII-A。抗RN 酶HII-A抗体(G-10)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。RNase HII-A,也称为RNASEH2A、RNASEHI、AGS4或RNHIA,是RNase HII复合物中关键的299个氨基酸催化亚基,通过降解RNA:DNA双链中的RNA,在DNA复制中发挥重要作用。RNase HII-A主要位于细胞核中,负责在DNA合成过程中从滞后链上移除冈崎片段RNA引物,确保新合成的DNA的完整性和连续性。RNase HII-A催化RNA的内切裂解,生成5'-磷酸单酯,需要镁或锰作为辅助因子才能发挥作用。编码RNase HII-A的基因突变与Aicardi-Goutieres综合征4型(AGS4)有关,这是一种严重的常染色体隐性遗传性脑病,会导致严重的神经系统损伤,包括脑萎缩和慢性脑脊液淋巴细胞增多,通常会导致儿童早期死亡。RNase HII-A与其他蛋白的相互作用及其在DNA复制中的关键功能凸显了其在细胞过程和疾病机理中的重要性,这使得RNase HII-A单克隆抗体(G-10)成为研究人员研究这些关键途径的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RNase HII-A 抗体 (G-10) 参考文献:

  1. 人类核糖核酸酶 H1 和 H2 参与了细胞对反义硫代磷酸酯寡核苷酸反应的变化。  |  ten Asbroek, AL., et al. 2002. Eur J Biochem. 269: 583-92. PMID: 11856317
  2. 酿酒酵母的 RNase H2 是由三种蛋白质组成的复合体。  |  Jeong, HS., et al. 2004. Nucleic Acids Res. 32: 407-14. PMID: 14734815
  3. 在缺乏 RnaseHI 或 PolI 的 Klenow 结构域的流感嗜血杆菌突变体中,四核苷酸重复序列的稳定性受到破坏。  |  Bayliss, CD., et al. 2005. Nucleic Acids Res. 33: 400-8. PMID: 15653640
  4. 编码核糖核酸酶 H2 亚基的基因突变导致艾卡迪-古蒂耶尔综合征,并可模拟先天性脑病毒感染。  |  Crow, YJ., et al. 2006. Nat Genet. 38: 910-6. PMID: 16845400
  5. 艾卡迪-古铁雷斯综合征的临床和分子表型。  |  Rice, G., et al. 2007. Am J Hum Genet. 81: 713-25. PMID: 17846997
  6. 克隆编码人 RNase HI 大亚基的 cDNA,人 RNase HI 是原核生物 RNase HII 的同源物。  |  Frank, P., et al. 1998. Proc Natl Acad Sci U S A. 95: 12872-7. PMID: 9789007

订购信息

产品名称产品编号规格价格数量收藏夹

RNase HII-A 抗体 (G-10)

sc-515475
200 µg/ml
$316.00

RNase HII-A (G-10): m-IgG Fc BP-HRP 套装

sc-531385
200 µg Ab; 10 µg BP
$354.00

RNase HII-A (G-10): m-IgGκ BP-HRP 套装

sc-525062
200 µg Ab, 40 µg BP
$354.00

RNase HII-A (G-10): m-IgG1 BP-HRP 套装

sc-544519
200 µg Ab; 20 µg BP
$354.00

RNase HII-A 抗体 (G-10) AC

sc-515475 AC
500 µg/ml, 25% agarose
$416.00

RNase HII-A 抗体 (G-10) HRP

sc-515475 HRP
200 µg/ml
$316.00

RNase HII-A 抗体 (G-10) FITC

sc-515475 FITC
200 µg/ml
$330.00

RNase HII-A 抗体 (G-10) PE

sc-515475 PE
200 µg/ml
$343.00

RNase HII-A 抗体 (G-10) Alexa Fluor® 488

sc-515475 AF488
200 µg/ml
$357.00

RNase HII-A 抗体 (G-10) Alexa Fluor® 546

sc-515475 AF546
200 µg/ml
$357.00

RNase HII-A 抗体 (G-10) Alexa Fluor® 594

sc-515475 AF594
200 µg/ml
$357.00

RNase HII-A 抗体 (G-10) Alexa Fluor® 647

sc-515475 AF647
200 µg/ml
$357.00

RNase HII-A 抗体 (G-10) Alexa Fluor® 680

sc-515475 AF680
200 µg/ml
$357.00

RNase HII-A 抗体 (G-10) Alexa Fluor® 790

sc-515475 AF790
200 µg/ml
$357.00

can this antibody detect mouse RnaseH2A?

Asked by: KKumar
Thank you for your question. This antibody has not yet been tested in mouse and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-12-30

Can I receive a FREE 10 µg sample of RNase HII-A (G-10): sc-515475 and RNase H1 (H-4) sc-376326 for initial testing although it has to be shipped to Australia. If yes, how long would it roughly take to be sent to Canberra/ Australia? Thank you

Asked by: Alex17
Thank you for your interest in RNase HII-A (G-10): sc-515475 and RNase H1 (H-4) sc-376326. We would be happy to provide you with samples to test. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com to arrange the samples.
Answered by: Technical Service
Date published: 2017-07-14

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with RNase HII-A (G-10): sc-515475?

Asked by: Randy McDonald
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-28
  • y_2025, m_8, d_28, h_5CST
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with Hela cell lysate in published research. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Anti-RNase HII_A Antibody ReviewThe Anti-RNase HII-A antibody worked really well for our purposes.
Date published: 2022-10-17
Rated 5 out of 5 by from Strong specific band on Western BlotI received this as a free sample to test. I would be happy to buy this based on the Western blot result I got. Whole cell lysate (20ug protein) for two WT HCT116 clones and two HCT116 RNASEH2A-KO clones was separated by SDS-PAGE and probed with this G-10 (sc-515475) antibody at 1/1,000 dilution. Strong specific bands show in the WT clones, whereas the KO clones show no signal in this MW range. There are some (strong) non-specific bands at higher MW, something to keep in mind when using this antibody for other appications. Seems an excellent antibody for the detection of human RNASEH2A by Western blotting.
Date published: 2017-12-08
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RNase HII-A 抗体 (G-10) is rated 5.0 out of 5 by 3.
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