Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ribosomal Protein S3 Double Nickase Plasmid (h): sc-402327-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ribosomal Protein S3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ribosomal Protein S3 Double Nickase Plasmid (h) and Ribosomal Protein S3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RPS3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ribosomal Protein S3 Antibody (C-7): sc-376008
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ribosomal Protein S3 Double Nickase Plasmid (h) | sc-402327-NIC | 20 µg | $410.00 | |||
Ribosomal Protein S3 Double Nickase Plasmid (h2) | sc-402327-NIC-2 | 20 µg | $410.00 |
RPS3 encodes human Ribosomal Protein S3, a core component of the 40S small ribosomal subunit required for accurate translation initiation and mRNA decoding. Beyond its structural role in ribosome biogenesis, RPS3 has been linked to cellular stress responses and regulation of signaling pathways that coordinate protein synthesis with growth and DNA damage surveillance. Altered ribosomal protein homeostasis can perturb translational control and proteostasis, processes frequently implicated in tumor biology and other disorders characterized by dysregulated cell proliferation. As a result, RPS3 is widely studied in the context of ribosome function, stress-adaptive transcriptional programs, and mechanisms connecting translation to genome integrity.
Ribosomal Protein S3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RPS3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RPS3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RPS3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RPS3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.