Ribosomal Protein L39 Double Nickase Plasmid (h): sc-410990-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ribosomal Protein L39 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ribosomal Protein L39 Double Nickase Plasmid (h) and Ribosomal Protein L39 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RPL39. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ribosomal Protein L39 Double Nickase Plasmid (h) | sc-410990-NIC | 20 µg | $410.00 | |||
Ribosomal Protein L39 Double Nickase Plasmid (h2) | sc-410990-NIC-2 | 20 µg | $410.00 |
Human RPL39 encodes ribosomal protein L39, a small basic component of the 60S large ribosomal subunit that contributes to ribosome assembly and stable translation of mRNAs. As part of the core translational machinery, RPL39 influences protein synthesis capacity, co-translational quality control, and broader cellular programs linked to growth, stress responses, and proteostasis. Perturbation of ribosomal proteins can elicit nucleolar stress and downstream signaling changes, including p53-dependent checkpoints, and may alter translational selectivity for specific transcripts. Dysregulation of ribosome biogenesis and function is widely implicated in ribosomopathies and cancer-related translational reprogramming, making RPL39 a relevant target for studying how altered ribosome composition impacts cell state and disease-associated phenotypes.
Ribosomal Protein L39 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RPL39 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RPL39. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RPL39 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RPL39-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.